Purification of superoxide dismutase from bacterial isolate: Superoxide dismutase purification - Couverture souple

Sharma, Vivek; Sharma, Sneh; Sharma, Kamal

 
9786203839692: Purification of superoxide dismutase from bacterial isolate: Superoxide dismutase purification
Couverture souple
ISBN 10 : 6203839698 ISBN 13 : 9786203839692
Editeur : LAP LAMBERT Academic Publishing, 2021

Synopsis

The cells were harvested from bacterial culture isolate by centrifugation and were suspended in Tris-HCl buffer of pH 8.0. The optical density (O.D.) of cells was measured at 600nm and each time cells of 21.5 optical densities (O.D.) were disrupted and used for the purification of the enzyme. Purification of superoxide dismutase from SPB-13. Ammonium sulphate precipitation method was used for concentrating the proteins. After this, the enzyme was further purified by DEAE-Sepharose column. The DEAE-sepharose column was loaded with 3ml of concentrated protein.The enzyme activity was only recorded in fractions ranging from 22, 23, 24, 25, 26, 27 and 28. These fractions were polled together. The polled fractions along with crude enzyme were checked for purity by SDS-PAGE.The maximum activity of superoxide dismutase of SPB-13 was found to be in Tris-HCl buffer, 60mM molarity, pH 8.0, incubation time was found to be 2.0 minutes, incubation temperature 450C. An enzyme concentration of 10µg was found to be optimum. Thermostability of superoxide dismutase was checked and enzyme was found to be thermostable upto 550C for 60 minutes.

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Éditeur
LAP LAMBERT Academic Publishing
Date d'édition
2021
Langue
anglais
ISBN 10 
6203839698
ISBN 13 
9786203839692
Reliure
Broché
Nombre de pages
68
Coordonnées du fabricant
non disponible
Personne responsable
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