Forensic STR analysis of compromised DNA: PCR multiplex system for simultaneous detection of 10 miniSTRs, SE33, Penta E, Penta D, and four Y-STRs - Couverture souple

Shafique, Muhammad

 
9786203923131: Forensic STR analysis of compromised DNA: PCR multiplex system for simultaneous detection of 10 miniSTRs, SE33, Penta E, Penta D, and four Y-STRs

Synopsis

Application of DNA testing has been developed into a requisite and regularcomponent of recent forensic casework, based on highly sensitive and stable PCR techniques for the analysis of different types of biological specimens. In the current study, development, optimization and validation of a multiplex system was accomplished comprising on 10 autosomal miniSTRs, SE33, Penta E, Penta D and four Y chromosomal STRs (DYS385a/b, DYS438 and DYS392) along with gender detection marker amelogenin into a single PCR reaction simultaneously.

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