Tissue Fixation and Processing: A Practical Overview - Couverture souple

Uppala, Divya; Balla, Hyandavi; Pamidimukkala, Sahithi

Couverture souple

ISBN 10 : 6206740420 ISBN 13 : 9786206740421

Editeur : LAP LAMBERT Academic Publishing, 2025

Synopsis

This comprehensive manual provides an in-depth exploration of tissue fixation, processing, and embedding techniques essential in histopathology and biomedical research. Beginning with the fundamental concepts of autolysis and fixation, the book delves into the chemistry and classification of various fixatives including aldehydes, oxidizing agents, protein denaturants, and cross-linking compounds highlighting their mechanisms, applications, and impact on tissue morphology and staining properties. It further addresses the physical and chemical factors affecting fixation, such as pH, temperature, penetration rate, and osmolality. Detailed discussions cover both conventional and advanced methods like microwave fixation and the use of secondary fixatives for enhanced preservation. The book also provides extensive guidance on tissue processing stages, including dehydration, clearing, impregnation, and embedding using paraffin, celloidin, resins, and ester wax. Special sections include techniques for photomicrography, image analysis, and tumor marker identification, offering valuable insights for diagnostic and research applications.

Détails de l'édition

• Éditeur: LAP LAMBERT Academic Publishing
• Date d'édition: 2025
• Langue: anglais
• ISBN 10: 6206740420
• ISBN 13: 9786206740421
• Reliure: Broché
• Nombre de pages: 224
• Coordonnées du fabricant: non disponible
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