Systematic Analysis of Working Models to Detect Thioredoxin Activities: Detecting Thioredoxin Activity by Systemic Analytical Tools - Couverture souple

Verma, Neha; Abbot, Vikrant; Sharma, Mrinal

 
9786206844068: Systematic Analysis of Working Models to Detect Thioredoxin Activities: Detecting Thioredoxin Activity by Systemic Analytical Tools
Couverture souple
ISBN 10 : 6206844064 ISBN 13 : 9786206844068
Editeur : LAP LAMBERT Academic Publishing, 2023

Synopsis

Thioredoxin (Trx) (12 kDa M.W.) is a ubiquitous antioxidant protein which controls the oxidative stress and redox signaling with thioredoxin reductase (TrxR) and NADPH. Trx acts as an electron donor for ribonucleotide reductase (RNR), methionine sulfoxide reductase (Msr), and Trx peroxidase (peroxiredoxin). Trx plays an important role in DNA synthesis, reduction of the disulfide bond, recycling of peroxiredoxin (Prx), and as an electron donor. Various in vivo and in vitro assays are used to check the activity of Trx. This work is focused on the study of in vivo and in vitro methods for Trx activity which have been used in the different model system. Different methods were studied to evaluate the activity of Trx in different samples. Insulin reduction assay is more commonly used in vitro method because insulin acts as effective substrate for activity of Trx. Fluorescence, DTNB (5, 5ʹ-dithiobis (2-nitrobenzoic acid)) reduction, spectrophotometric or micro-method insulin reduction, and disulfide cross-linking interaction in vitro assays while Luciferase, cloning, and wort in vivo assays are used for the activity of the Trx system.

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Éditeur
LAP LAMBERT Academic Publishing
Date d'édition
2023
Langue
anglais
ISBN 10 
6206844064
ISBN 13 
9786206844068
Reliure
Broché
Nombre de pages
52
Coordonnées du fabricant
non disponible
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