A Practical Guide to Influenza Virus Isolation and Identification - Couverture souple

Boora, Sanjit; Khan, Anish; Kaushik, Samander

 
9786209309304: A Practical Guide to Influenza Virus Isolation and Identification
Couverture souple
ISBN 10 : 6209309305 ISBN 13 : 9786209309304
Editeur : LAP LAMBERT Academic Publishing, 2025

Synopsis

This practical guide details the essential methodologies for the isolation, identification, and quantification of Influenza A viruses, specifically focusing on the H1N1pdm09 strain using MDCK cell culture systems. It provides a comprehensive workflow that includes standardized protocols for the maintenance and subculturing of MDCK cells, viral propagation techniques, and molecular confirmation via RT-PCR targeting the HA gene. Furthermore, the text offers in-depth instruction on viral quantification methods, employing Hemagglutination (HA) assays to determine total viral titers and TCID50 assays using the Reed-Muench method for calculating infectious titers. By combining theoretical background with rigorous experimental data and step-by-step procedural flowcharts, this work serves as a vital resource for researchers and laboratory professionals establishing robust platforms for influenza virus characterization and antiviral drug discovery.

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Éditeur
LAP LAMBERT Academic Publishing
Date d'édition
2025
Langue
anglais
ISBN 10 
6209309305
ISBN 13 
9786209309304
Reliure
Broché
Nombre de pages
52
Coordonnées du fabricant
non disponible
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