The Hepatocyte Review - Couverture souple

Couverture souple

ISBN 10 : 9048154022 ISBN 13 : 9789048154029

Editeur : Springer, 2010

Synopsis

It is thirty years since the technique of high-yield preparation of isolated hepatocytes, by collagenase perfusion of the liver, was published. The original method described by Berry and Friend has undergone many minor modifications by other workers, and the two-step procedure introduced by Seglen in 1976 has become the most frequent way to prepare hepatocyte suspensions. An important development introduced by Bissell in 1973 was the use of the cells as the first step in monolayer culture. The availability of the isolated hepatocyte preparation as cells in suspension or culture has undoubtedly facilitated research on the liver. This was emphasised in our book, published (with Dr. Greg Barritt) in 1990, which described in detail methods of preparation and the properties of the isolated hepatocytes. It also discussed the usefulness of the preparation for the study of intermediary and xenobiotic metabolism, calcium ion transport, and the growth and differentiation of hepatocytes in culture. The book also touched briefly on a range of specialised techniques, including peri fusion, subcellular fractionation, transplantation, cryopreservation and measurement of intracellular pH. Although standard procedures for the manipulation of hepatocytes have not changed a great deal in ten years, they have undoubtedly been refined. This applies particularly to hepatocyte culture techniques, cryopreservation, and even to preparation of hepatocyte suspensions, where it is now feasible to use purified enzymes. There is also much more emphasis on the use and study of human hepatocytes, particularly in the field of pharmacology and therapeutics.

A propos du livre

Présentation de l'éditeur

The isolated hepatocyte preparation, employed in suspension or culture, is now firmly established as a major tool not only for the study of the liver but also for the investigation of a large number of aspects of biology common to many cell types. The Hepatocyte Review provides a comprehensive description of recent advances in methodology and biology pertaining to the isolated hepatocyte preparation, and offers a unique insight into the value of isolated hepatocytes for basic and applied studies. A particular feature is the series of major reviews on growth regulation by hormones and growth factors, cell cycle progression and active cell death. The book also assembles for the first time novel work on the use of hepatocytes for clinical applications in the fields of transplantation, gene therapy and the bioartificial liver. Another important feature is a compilation of methods for isolated hepatocyte preparation from animals other than the rat, and includes several accounts of the isolation of human hepatocytes, which is becoming an increasingly important technique. A number of specialised techniques for hepatocyte manipulation, and a detailed description of new advances in hepatocyte culture, are also included. The Hepatocyte Review is essential reading for any scientist using, or contemplating using, the isolated hepatocyte technique and will also be of major interest to cell biologists in general.

Détails de l'édition

• Éditeur: Springer
• Date d'édition: 2010
• Langue: anglais
• ISBN 10: 9048154022
• ISBN 13: 9789048154029
• Reliure: Broché
• Nombre de pages: 624
• Éditeur: Berry M. N., Edwards Anthony M.
• Coordonnées du fabricant: Springer Nature Customer Service Center GmbH; ProductSafety@springernature.com; ; Europaplatz 3,69115 Heidelberg, Germany; Heidelberg; 69115; Allemagne