The Chick Embryo Chorioallantoic Membrane in the Study of Angiogenesis and Metastasis: The CAM assay in the study of angiogenesis and metastasis - Couverture souple

Ribatti, Domenico

9789400791572: The Chick Embryo Chorioallantoic Membrane in the Study of Angiogenesis and Metastasis: The CAM assay in the study of angiogenesis and metastasis

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ISBN 10 : 9400791577 ISBN 13 : 9789400791572

Editeur : Springer, 2014

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1. Introduction 2. Chorioallantoic membrane and its embryological origin 2.1. Morphology of chorioallantoic membrane blood and lymphatic vessels 2.2. A single blood sinus or a capillary plexus beneath the chorionic epithelium? 2.3. The chorioallantoic membrane vascular growth 3. Use of chorioallantoic membrane in the study of angiogenic molecules 3.1. Role of FGF-2 in chorioallantoic membrane vascularization 4. Use of chorioallantoic membrane in the study of antiangiogenic molecules 5. Use of chorioallantoic membranes in the study of tumor angiogenesis 5.1. Angiogenesis and antiangiogenesis in multiple myeloma 5.2. Angiogenesis and antiangiogenesis in human neuroblastoma 6. Use of chorioallantoic membrane in the study of tumor metastasis 6.1. Spontaneous metastasis models. 6.2. Experimental metastasis studies. 7. Other applications 7.1. In ovo and ex ovo methods 7.2. Use of chorioallantoic membrane in the study of tumor lymphangiogenesis 8. Different morphological techniques that can be used to study vascularization of the CAM and the genes involved. 9. Methods of quantifying the angiogenic response 10. Limitations of the chorioallantoic membrane assay 11. Other classical in vivo assays in comparison (advantages, disadvantages and limitations) with the chorioallantoic membrane 12. Concluding remarks Acknowledgments References

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�diteur: Springer
Date d'�dition: 2014
Langue: anglais
ISBN 10: 9400791577
ISBN 13: 9789400791572
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Nombre de pages: 136
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The Chick Embryo Chorioallantoic Membrane in the Study of Angiogenesis and Metastasis (eng)

Ribatti, Domenico

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The Chick Embryo Chorioallantoic Membrane in the Study of Angiogenesis and Metastasis: The CAM assay in the study of angiogenesis and metastasis

Ribatti, Domenico

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The Chick Embryo Chorioallantoic Membrane in the Study of Angiogenesis and Metastasis

Domenico Ribatti

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Taschenbuch. Etat : Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -As pointed out by Auerbach in 1991 Perhaps the most consistent limitation to progress in angiogenesis research has been the availability of simple, reliable, reproducible, quantitative assays of the angiogenesis response. In vitro angiogenesis assays, based on endothelial cell cultures or tissue explant, focus on isolated endothelial cell functions (e.g., endothelial cell proliferation, migration, or invasion) and do not examine the coordination of cell functions required for a successful angiogenic response (Jain et al., 1997; Auerbach et al., 2000). Although in vitro angiogenesis assays have been useful for identi cation of potential molecular targets to block endothelial cell responses and preliminary screening of novel pharmacological agents, they frequently cannot be correlated with in vivo angiogenesis measurements. This is most likely the result of the c- plex and multiple cellular mechanism evoked during new blood vessel formation in vivo. In vitro assays cannot be considered conclusive and the activity of a compound must be con rmed in other assays of increasing complexity, including in vivo assays of angiogenesis, angiogenic-dependent tumor growth, and metastasis. 136 pp. Englisch. N� de r�f. du vendeur 9789400791572

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The Chick Embryo Chorioallantoic Membrane in the Study of Angiogenesis and Metastasis

Domenico Ribatti

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Etat : New. Dieser Artikel ist ein Print on Demand Artikel und wird nach Ihrer Bestellung fuer Sie gedruckt. Several in vivo assays are currently used in the study of angiogenesis and antiangiogenesisThe chick embryo chorioallantoic membrane is one of the most common and versatile assay to study angiogenesis and antiangiogenesis in vivoAngiogenesi. N� de r�f. du vendeur 11511148

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The Chick Embryo Chorioallantoic Membrane in the Study of Angiogenesis and Metastasis | The CAM assay in the study of angiogenesis and metastasis

Domenico Ribatti

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The Chick Embryo Chorioallantoic Membrane in the Study of Angiogenesis and Metastasis

Domenico Ribatti

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ISBN 10 : 9400791577 ISBN 13 : 9789400791572

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Taschenbuch. Etat : Neu. This item is printed on demand - Print on Demand Titel. Neuware -As pointed out by Auerbach in 1991 �Perhaps the most consistent limitation to progress in angiogenesis research has been the availability of simple, reliable, reproducible, quantitative assays of the angiogenesis response.� In vitro angiogenesis assays, based on endothelial cell cultures or tissue explant, focus on isolated endothelial cell functions (e.g., endothelial cell proliferation, migration, or invasion) and do not examine the coordination of cell functions required for a successful angiogenic response (Jain et al., 1997; Auerbach et al., 2000). Although in vitro angiogenesis assays have been useful for identi cation of potential molecular targets to block endothelial cell responses and preliminary screening of novel pharmacological agents, they frequently cannot be correlated with in vivo angiogenesis measurements. This is most likely the result of the c- plex and multiple cellular mechanism evoked during new blood vessel formation in vivo. In vitro assays cannot be considered conclusive and the activity of a compound must be con rmed in other assays of increasing complexity, including in vivo assays of angiogenesis, angiogenic-dependent tumor growth, and metastasis.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 136 pp. Englisch. N� de r�f. du vendeur 9789400791572

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The Chick Embryo Chorioallantoic Membrane in the Study of Angiogenesis and Metastasis : The CAM assay in the study of angiogenesis and metastasis

Domenico Ribatti

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ISBN 10 : 9400791577 ISBN 13 : 9789400791572

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Taschenbuch. Etat : Neu. Druck auf Anfrage Neuware - Printed after ordering - The chick embryo chorioallantoic membrane (CAM) is an extraembryonic membrane which serves as a gas exchange surface and its function is supported by a dense capillary network. Because of its extensive vascularization and easy accessibility, the CAM has been broadly used to study the morpho-functional aspects of the angiogenesis process in vivo and to investigate the efficacy and mechanisms of action of pro-angiogenic and anti-angiogenic natural and synthetic molecules. The CAM is a suitable site for transplanting tissues, which can survive and develop in the CAM by peripheral anastomoses between graft and original CAM vasculature or by new angiogenic vessels grown from the CAM that invade the graft. While the formation of peripheral anastomoses between host and pre-existing donor vessels is the main, and the most common, mechanism involved in the revascularization of embryonic grafts, the growth of CAM-derived vessels into the graft is only stimulated in tumor grafts. The CAM has long been a favored system for the study of tumor angiogenesis and metastasis, because at this stage the chick immunocompetence system is not fully developed and the conditions for rejection have not been established. Tumors remain avascular for 72 h, after which they are penetrated by new blood vessels and begin a phase of rapid growth. Also, delivery of tumor cells onto the CAM allows the fine study of the effects of tumor derived angiogenic growth factors on blood vessel structure and functionality. The CAM may also used to verify the ability to inhibit the growth of capillaries by implanting tumors onto the CAM and by comparing tumor growth and vascularization with or without the administration of an anti-angiogenic molecule. Other studies using the tumor cells/CAM model have focused on the invasion of the chorionic epithelium and the blood vessels by tumor cells. The cells invade the epithelium and the mesenchymal connective tissue below, where they are found in the form of a densebed of blood vessels, which is a target for intravasation. N� de r�f. du vendeur 9789400791572

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