Calcium Entry Blockers in Cardiovascular and Cerebral Dysfunctions - Couverture souple

Couverture souple

ISBN 10 : 9400960352 ISBN 13 : 9789400960350

Editeur : Springer, 2011

Synopsis

2 The free internal Ca+ concentration in human red cells is set according to the leak- 2 and-pump principle: There is a finite passive Ca+ influx at the physiological 2 2 Ca+ -gradient across the membrane which is compensated by Ca+ pumping in the outward direction with a rate given by the degree of saturation of the A TP-fuelled Ca- 2 pump at the steady-state internal Ca+ concentration. Simons (1982) recently devised a method allowing the measurement of the steady- 2 2 state internal Ca+ concentration. Cells are suspended in media of different Ca+ con- 2 2 tent whose Ca+ concentration is monitored by a Ca+ -selective electrode. When the cells are lysed (by digitonin) there is an upward or downward deflection of the elec- 2 trode signal. At the point of zero deflection, the cellular Ca+ concentration equals that 2 of the medium. The result is, that in fresh human red blood cells the Ca+ concentra- tion is;;;; O.4, uM (this is an upper estimate; the true value may be considerably lower).

Détails de l'édition

• Éditeur: Springer
• Date d'édition: 2011
• Langue: anglais
• ISBN 10: 9400960352
• ISBN 13: 9789400960350
• Reliure: Broché
• Nombre de pages: 336
• Éditeur: Godfraind T., Herman A.G., Wellens D.
• Coordonnées du fabricant: ProductSafety@springernature.com; ProductSafety@springernature.com; ; Poststr. 9; Darmstadt; 64293; Allemagne