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The HPLC Survival Guide: The Lab Detective’s Handbook - Couverture souple

Livre 14 sur 15: Practical Pharma Science? - Applied Knowledge for Pharma Professionals

Gorjanu, Mirela

 
9798175215244: The HPLC Survival Guide: The Lab Detective’s Handbook

Synopsis

When your HPLC method goes wrong, know where to look next.

A blank shows a peak. Pressure suddenly rises. Your peaks start tailing. Two compounds co-elute. Retention time drifts. Your gradient behaves differently than expected.

What do you check first?

The HPLC Survival Guide: The Lab Detective's Handbook is a practical field manual for diagnosing and troubleshooting real-world reversed-phase HPLC problems in pharmaceutical QC and method development. Instead of academic theory alone, it uses a problem-first, diagnostic approach: recognise the symptom, investigate the likely causes, and decide the right next action.

14 field missions, including:

  • Ghost peaks, baseline noise and backpressure
  • Peak shape problems, stationary-phase selection and mobile-phase control
  • First-run method development and retention-time drift
  • Resolution, co-elution and gradient problems
  • UV/DAD detection, calibration and system suitability
  • Peak integration, sample and injection effects
  • Method transfer and robustness

Plus practical reference material, troubleshooting tools and checklists for interpreting chromatographic behaviour.

Who it's for: QC analysts, method development scientists, lab technicians and supervisors — anyone who needs to move from "What happened?" to "What should I check next?"

Stop guessing. Start diagnosing.

Part of the Practical Pharma Science™ series — applied knowledge for pharma professionals.

— Mirela Gorjanu, Practical Pharma Science™

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