ASCP HT Exam: Practice Questions - Couverture souple

Hewlett-Powell, Robin

Couverture souple

ISBN 13 : 9798776498954

Editeur : Independently published, 2021

Synopsis

ASCP HT Practice Exams: Prepare for Success!

This unofficial book contains 250 practice questions for the ASCP Histotechnician (HT) exam. The questions cover all five content areas on the ASCP HT exam:
1. Fixation
2. Processing
3. Embedding/microtomy
4. Staining
5. Laboratory operations

Many questions come with detailed explanations, helping you understand the answers and the reasoning behind them.

KEY FEATURES
• 250 multiple-choice practice questions, with answers and explanations
• Valuable information about the exam
• An index to help you more easily find information
Overall, this book is a must-have resource for anyone looking to pass the ASCP HT exam.

---SAMPLE QUESTIONS---

Here are five sample questions from the book:

1) FIXATION
How much water must be added to 100 mL of pure bleach to make 10% bleach?
a. 700 mL
b. 800 mL
c. 900 mL
d. 1,000 mL

Answer: c. 900 mL
The dilution formula is
c1 × v1 = c2 × v2
where:
c1 is the initial concentration
v1 is the initial volume
c2 is the final concentration
v2 is the final volume

Using the dilution formula:
c1 × v1 = c2 × v2
100 × 100 = 10 × v2
10,000 = 10 × v2
10,000/10 = v2
1,000 = v2

Therefore, the volume of the 10% bleach solution needs to be 1,000 mL. However, remember that 100 mL of this will be bleach, so to get the volume of water needed, we need to subtract 100 mL from 1,000 mL:
1,000 mL − 100 mL = 900 mL

2) PROCESSING
Which type of cell do anti-CD3 antibodies identify in immunohistochemistry?
a. B cell
b. Mast cell
c. Plasmacytoid dendritic cell
d. T cell

Answer: d. T cell
Explanation: CD3 is a defining feature of T cells. Therefore, anti-CD3 antibodies can be used to identify T cells.

3) EMBEDDING/MICROTOMY
What is the most widely used medium for infiltration and embedding?
a. Formic acid
b. Paraffin wax
c. Permount
d. Xylene

Answer: b. Paraffin wax
Explanation: Paraffin wax is the preferred way to infiltrate and embed specimens because it is inexpensive, easy to obtain, and compatible with a wide range of staining methods. It is also relatively easy to section and produces good-quality slides with clear tissue detail.

4) STAINING
Which Gram stain reagent is a mordant that binds the stain to the bacteria?
a. Acetone
b. Ethanol
c. Iodine
d. Safranin

Answer: c. Iodine
Explanation: The four basic reagents of Gram staining are crystal violet, iodine, ethanol (or acetone), and safranin. The iodine acts as a mordant, binding the stain to the bacteria.

5) LABORATORY OPERATIONS
What is a molar solution?
a. 1 gram of solute in 1 mL of solution
b. 1 gram of solute in 1 mole of solution
c. 1 mL of solute in 1 mole of solution
d. 1 mole of solute in 1 liter of solution

Answer: d. 1 mole of solute in 1 liter of solution
Explanation: A molar solution, often denoted as "M", is a solution that contains one mole of solute dissolved in a sufficient amount of solvent to make one liter of the solution.

Détails de l'édition

• Éditeur: Independently published
• Date d'édition: 2021
• Langue: anglais
• ISBN 13: 9798776498954
• Reliure: Broché
• Nombre de pages: 108
• Coordonnées du fabricant: Manufactured by Amazon on behalf of the author; https://www.amazon.fr/hz/contact-us; ; c/o Amazon Media EU S.à.r.l., 38 Avenue John F. Kennedy; Luxembourg; L-1855; Luxembourg