Application of DNA Microarray Technology for Wastewater Analysis (Paperback)

Langue : anglais

Edité par IWA Publishing, London, 2004

1843397277 / 9781843397274

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Paperback. Deoxyribonucleic acid (DNA) microarrays are widely used for differential expression studies and for detection of virulence genes in pure bacterial cultures. Their use in complex microbial samples, such as soil and wastewater, has been relatively less studied. This report presents the results of a systematic effort to apply DNA microarrays to pathogen detection and to bacterial source tracking (BST) in wastewater. Parameters such as the method of DNA extraction from the samples, the type of immobilized probe (whether polymerase chain reaction [PCR] amplicons or oligonucleotides), the length and method of immobilization of oligonucleotides, the method of DNA labelling, the combination of PCR amplification with microarray hybridization and the choice of PCR targets have been optimized. Results indicate that the combination of PCR followed by microarray hybridization can detect pathogens in wastewater samples down to a 0.1% detection limit. The use of microarrays for bacterial source tracking gave promising results on human samples, however the probes used in this study only provided signals for general indicators of fecal contamination when used on samples of animal origin. The overall conclusion is that microarray technology has not yet reached the stage of routine use for microbiological analysis of wastewater. This report demonstrates: That the hybridization of total genomic DNA on microarrays has a high detection limit, of the order of 10^7 genomes; That the use of long oligonucleotides or PCR amplicons from 16S rDNA, or cpn60 probes has insufficient specificity to differentiate several important pathogens, especially within the Enterobacteriaceae family; That the use of short oligonucleotide immobilized probes coupled with PCR amplification of conserved genes, such as 16S rDNA, cpn60, or wecE, can detect pathogens in wastewater down to a 0.1% (DNA weight/weight) concentration; and The potential of DNA microarrays in BST, even if further research work remains necessary to achieve this goal. Deoxyribonucleic acid (DNA) microarrays are widely used for differential expression studies and for detection of virulence genes in pure bacterial cultures. Their use in complex microbial samples, such as soil and wastewater, has been relatively less studied. This report presents the results of a systematic effort to apply DNA microarrays to pathogen detection and to bacterial source tracking (BST) in wastewater. Parameters such as the method of DNA extraction from the samples, the type of immobilized probe (whether polymerase chain reaction [PCR] amplicons or oligonucleotides), the length and method of immobilization of oligonucleotides, the method of DNA labelling, the combination of PCR amplification with microarray hybridization and the choice of PCR targets have been optimized. Shipping may be from our Sydney, NSW warehouse or from our UK or US warehouse, depending on stock availability.…

N° de réf. du vendeur 9781843397274

Titre
Application of DNA Microarray Technology for Wastewater Analysis (Paperback)
Auteur
F. Berthiaume
Éditeur
IWA Publishing, London
Année de publication
2004
État de l'article
new
Reliure
Paperback
Langue
anglais
ISBN à 10 chiffres
1843397277
ISBN à 13 chiffres
9781843397274

AussieBookSeller

Truganina, VIC, Australie

Vendeur avec une évaluation de 5 étoiles

Vendeur AbeBooks depuis 22 juin 2007

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Article25 à 45 jours ouvrés8 à 14 jours ouvrés
Premier articleEUR 32,89EUR 39,12
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