Thin-layer chromatography (TLC) is a powerful, fast and inexpensive analytical method. It has proven its usefulness in pharmaceutical, food and environmental analysis. With this new book both new comers and experienced practitioners can make full use of these advantages of TLC. All steps of the analytical procedure are clearly explained, starting with the choice of a suitable TLC technique and ending with data evaluation and documentation. Special emphasis is put on the proper choice of materials for TLC. Properties and functions of various materials and the TLC equipment are described, covering e. g. precoated layers, solvents and developing chambers, and including information on suppliers. Many practical hints for trouble shooting are given. All this is illustrated with numerous coloured figures. How to use TLC in compliance with GLP/GMP regulations is described in detail, including the required documentation. Therefore the reader can very easily compile his own standard operating procedures.
Thin–layer chromatography (TLC) is a powerful, fast and inexpensive analytical method, which has proven its effectiveness in the analysis of pharmaceuticals, food and the environment. This new edition of the practical guide to TLC includes a completely revised chapter on documentation, which now covers the use of digital cameras, while selected new sorbents and instruments are also introduced.
This edition retains the successful features of its predecessor:
- Clear explanation of all steps in the analytical procedure, starting with the choice of a suitable TLC technique and finishing with data evaluation and documentation.
- Special emphasis on the correct choice of materials for TLC. Properties and functions of various materials and the TLC equipment are described, covering precoated layers, solvents and developing chambers, including information on suppliers, among others.
- Many practical hints for troubleshooting.
- Detailed description of using TLC in compliance with GLP/GMP regulations, including the required documentation , allowing readers to easily compile their own standard operating procedures.
- Numerous color illustrations.
From the reviews of the first edition:
"A clear, step–by–step practical guide to TLC, covering every stage of the process from plate selection and sample application to development and quantification. At every stage potential pitfalls are clearly signposted and each chapter contains a profusion of pracitcal tips. Even a seasoned TLC practitioner should find something of use or interest in this book which complements most existing texts."
The Analyst