Binding Between Protein and Drugs and Second Derivatives Application

Shihata, Ahmed Mohamed Ahmed/ Abdel-Aziz Y. El-Sayed, Ahmed Omran

ISBN 10: 3330033576 / ISBN 13: 9783330033573

Edité par LAP LAMBERT Academic Publishing, 2017

184 pages. 8.66x5.91x0.42 inches. In Stock. N° de réf. du vendeur __3330033576

Synopsis :

Binding of a drug to a large molecule in the tissues or fluids, e.g. binding to the protein in the blood, may affect the metabolism of the drug, especially its rate of excretion. Various methods have been used for the determination of drug-protein binding constants. Binding constants of drugs to proteins can be obtained by analyzing the absorbance difference before and after the addition of protein to aqueous drug solutions. But background signals caused by the protein are so strong that the baseline is not always flat and zero even if reference solutions are used with the same amount of protein. Thus, these absorption changes of the drugs are not usually accurate enough for direct calculation of the binding constants. The present work explains the binding between drugs and protein and explores further validation of the second derivative method in the investigation of binding off, the widely administrated drugs to bovine serum albumin (BSA).

Présentation de l'éditeur: Binding of a drug to a large molecule in the tissues or fluids, e.g. binding to the protein in the blood, may affect the metabolism of the drug, especially its rate of excretion. Various methods have been used for the determination of drug-protein binding constants. Binding constants of drugs to proteins can be obtained by analyzing the absorbance difference before and after the addition of protein to aqueous drug solutions. But background signals caused by the protein are so strong that the baseline is not always flat and zero even if reference solutions are used with the same amount of protein. Thus, these absorption changes of the drugs are not usually accurate enough for direct calculation of the binding constants. The present work explains the binding between drugs and protein and explores further validation of the second derivative method in the investigation of binding off, the widely administrated drugs to bovine serum albumin (BSA).

Détails bibliographiques

Titre : Binding Between Protein and Drugs and Second...
Éditeur : LAP LAMBERT Academic Publishing
Date d'édition : 2017
Reliure : Paperback
Etat : Brand New