CRISPR-Cas Methods

M. Tofazzal Islam

ISBN 10: 1071606182 / ISBN 13: 9781071606186

Edité par Springer US, Springer US Jul 2020, 2020

A propos de cet article

Neuware -This volume details the fundamentals of the CRISPR-Cas system, and its protocols illustrate advances in CRISPR-Cas techniques for efficient genome editing. Introductory chapters provide a wide horizon of CRISPR/Cas-based methods and applications. Additional chapters guide readers through HDR-mediated editing, sgRNA design, the step-by-step procedure of multiplex adenine base editing experiments in rice, generating mutants for rice, wheat, Brachypodium, Barley, Flax, and Phytophthora, visual screening of mutants, gene deletion (knock-out), tagging (knock-in) in mammalian cells, the cloning-free (DNA-free) technique, cell-penetrating peptides, generating a genome-edited banana, and nuclear genome editing of Chlamydomonas employing CRISPR-Cpf1 combined with a single-stranded DNA (ssODN) repair template.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 296 pp. Englisch.

N° de réf. du vendeur 9781071606186

Détails bibliographiques

• Titre: CRISPR-Cas Methods
• Éditeur: Springer US, Springer US Jul 2020
• Date de parution: 2020
• Langue: anglais
• ISBN à 10 chiffres: 1071606182
• ISBN à 13 chiffres: 9781071606186
• Reliure: Taschenbuch
• État de l'article: Neu
• Dimensions: 254 L x 178 H x 17 P (en millimètres) 254 millimètres de largeur par 178 millimètres de hauteur par 17 millimètres de profondeur
• Poids: 561 grammes

A propos de ce titre

Synopsis

This volume details the fundamentals of the CRISPR-Cas system, and its protocols illustrate advances in CRISPR-Cas techniques for efficient genome editing. Introductory chapters provide a wide horizon of CRISPR/Cas-based methods and applications. Additional chapters guide readers through HDR-mediated editing, sgRNA design, the step-by-step procedure of multiplex adenine base editing experiments in rice, generating mutants for rice, wheat, Brachypodium, Barley, Flax, and Phytophthora, visual screening of mutants, gene deletion (knock-out), tagging (knock-in) in mammalian cells, the cloning-free (DNA-free) technique, cell-penetrating peptides, generating a genome-edited banana, and nuclear genome editing of Chlamydomonas employing CRISPR-Cpf1 combined with a single-stranded DNA (ssODN) repair template.
Authoritative and cutting-edge, CRISPR-Cas Methods aims to assist researchers who are new to the field and are aiming to learn how best to adopt this technology for a particular organism.

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