Chromatin Protocols

Srikumar P. Chellappan

ISBN 10: 1493944061 / ISBN 13: 9781493944064

Edité par Springer US, Springer New York Okt 2016, 2016

A propos de cet article

Neuware -Updated and revised, this thorough volume is organized such that it begins with techniques related to the study of chromatin structure. Protocols for reconstitution of chromatin on solid supports for analysis, preparation of positioned mononucleosomes, techniques to study premature chromatin condensation and the use of comparative genomic hybridization to assess genomic aberration are included as well. Novel techniques for imaging chromatin using atomic force microscopy and the isolation of specific genomic regions using engineered DNA binding molecules generated by CRISPR are then examined. That section is followed by protocols to analyze DNA and histone modifications, while the third section includes methods to study DNA replication and repair, in the context of chromatin.Last but not least, protocols for studying chromatin and its relation with transcriptional regulation are presented in a fourth section. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.Authoritative and up-to-date, Chromatin Protocols, Third Edition aims to help researchers in facilitating in-depth molecular analysis of various aspects of chromatin structure and function.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 508 pp. Englisch.

N° de réf. du vendeur 9781493944064

Détails bibliographiques

• Titre: Chromatin Protocols
• Éditeur: Springer US, Springer New York Okt 2016
• Date de parution: 2016
• Langue: anglais
• ISBN à 10 chiffres: 1493944061
• ISBN à 13 chiffres: 9781493944064
• Reliure: Taschenbuch
• État de l'article: Neu
• Dimensions: 254 L x 178 H x 26 P (en millimètres) 254 millimètres de largeur par 178 millimètres de hauteur par 26 millimètres de profondeur
• Poids: 1060 grammes

A propos de ce titre

Synopsis

Updated and revised, this thorough volume is organized such that it begins with techniques related to the study of chromatin structure. Protocols for reconstitution of chromatin on solid supports for analysis, preparation of positioned mononucleosomes, techniques to study premature chromatin condensation and the use of comparative genomic hybridization to assess genomic aberration are included as well. Novel techniques for imaging chromatin using atomic force microscopy and the isolation of specific genomic regions using engineered DNA binding molecules generated by CRISPR are then examined. That section is followed by protocols to analyze DNA and histone modifications, while the third section includes methods to study DNA replication and repair, in the context of chromatin. Last but not least, protocols for studying chromatin and its relation with transcriptional regulation are presented in a fourth section. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.

Authoritative and up-to-date, Chromatin Protocols, Third Edition aims to help researchers in facilitating in-depth molecular analysis of various aspects of chromatin structure and function.