Clinical Applications of Biomolecules in Disease Diagnosis

This textbook introduces the clinical applications of biomolecules in disease diagnosis, covering a range of topics in biochemistry. The book discusses carbohydrate and protein metabolism abnormalities, lipid digestion and absorption defects, disorders of purine and pyrimidine metabolism, and the chemistry and biological roles of water- and fat-soluble vitamins. It also covers the roles of different macroelements and essential trace elements in physiological processes and the prevention of chronic diseases. The final chapter focuses on recent developments in our understanding of the role of enzymes in disease development and diagnosis. The book chapter also explores the causes and effects of free radicals in the body, including oxidative stress and diseases such as cardiovascular and neurodegenerative diseases. Finally, the chapter discusses cancer development, causes, diagnosis methods, biomarkers, and current treatments such as targeted drug therapy, chemotherapy, and radiation therapy. This book is a useful resource for undergraduate and postgraduate students, researchers and practitioners in biochemistry, medicine, and health sciences.

Professor Ram Lakhan Singh is presently holding the position of Professor of Biochemistry at Dr. Rammanohar Lohia Avadh University, Ayodhya, UP. He has shaped Nilamber-Pitamber University, Medininagar, a state University of Jharkhand, as its Vice-Chancellor for a tenure of 3 years (June 2020 to June 2023). Professor Singh is having more than 36 years of teaching, research and administrative experience of which 22 years as Professor of Biochemistry. His main areas of research are Clinical/Nutraceutical Biochemistry, Environmental Biotechnology and Toxicology. He published 93 research papers in National and International journals of repute and guided 26 students for their Ph.D. degrees. Professor Singh edited 11 books and contributed 23 chapters in various books published by International publishers. He is on the panel of experts in various academic bodies and selection committees of various universities and funding agencies. He has been honored as Best Teacher by International Association of Lions Clubs in 1999. Professor Singh has been awarded IUTOX Senior Fellowship by International Union of Toxicology during XI International Congress of Toxicology at Montreal, Canada in 2007. He has been conferred with ‘Shikshak Shree Samman’ by Government of Uttar Pradesh in 2012 and admitted to the Fellowships of the Society of Toxicology, India in 2011 and Academy of Environmental Biology, India in 2015. He has been conferred with ‘Lifetime Achievement Award’ by Society of Life Sciences, India (2018) and Astha Foundation, India (2022).

Dr. Pankaj Singh is presently working as Assistant Professor in the Department of Biotechnology, Dr. Rammanohar Lohia Avadh University, Ayodhya, since 2019. He has more than nine years teaching experiences at graduate and post graduate levels. He completed his master’s degree in Biochemistry from Dr. Rammanohar Lohia Avadh University, Ayodhya, in 2007, and was awarded Research Fellowship under the UGC program of “Research Fellowship in Sciences for Meritorious Students” from March 2008 to March 2013. He joined the Department of Biochemistry at Dr. Rammanohar Lohia Avadh University, Ayodhya as Ph.D. scholar in 2008, and worked extensively on antioxidant and hepatoprotective activities of medicinal plants and awarded Ph.D. degree in 2014. His research interest is in Nutraceutical/Clinical Biochemistry, Pharmacology and Toxicology. He has published 38 research papers in peer-reviewed journals and contributed 11 chapters in various books published by International publishers. He participated in various national and international scientific conferences and symposia and presented 12 research papers. He has also participated in four national training programmes related to molecular analysis, identification of phytochemicals and faculty development program.

Prof. Neelam Pathak is currently working as a Professor at the Department of Biochemistry and Dean Students’ Welfare at Dr. Rammanohar Lohia Avadh University, Ayodhya, India. Earlier she has worked as a Professor at the Department of Biosciences, Integral University, Lucknow, India. She has a master’s in Biochemistry from ANDUAT, Ayodhya India, and a Ph.D. in Biochemistry from the University of Lucknow, Lucknow, India. After her doctoral degree, she worked as Research Associate at the Plant gene expression Lab, NBRI, Lucknow. Thereafter she has also worked as a Faculty Research Associate at the University of Maryland, College Park USA. She has received several fellowships (CSIR/UGC- JRF, SRF during her Ph.D. and CSIR- RA Award and Pool Officer Fellowship at post-Ph.D. level. She has also been the recipient of the Young Scientist Fast Track Award from the DST Govt. of India.  Prof. Pathak has ~20 years of teaching experience in various undergraduate and postgraduate life sciences courses. Moreover, she has ~30 years of research experience in plant biochemistry and molecular biology. She is a recipient of several research schemes sponsored by various funding agencies. Her research focuses on fruit ripening, medicinal plants, plant biodiversity, and plant growth-promoting microorganisms. She has co-authored a book on Genetic Engineering" which is one of the bestsellers in the life sciences category of Oxford University, Press. She has published 90 research papers in international journals of high repute. She has also authored 13 book chapters. She has presented papers at various national and international conferences and received several best and eminent scientist awards. She is a fellow of ISHRD and has been conferred with various prestigious awards.