Dengue Virus : Methods and Protocols

Ronaldo Mohana-Borges

ISBN 10: 1071618784 / ISBN 13: 9781071618783

Edité par Springer US, Springer US, 2021

A propos de cet article

Druck auf Anfrage Neuware - Printed after ordering - This detailed volume explores various aspects of the dengue virus and its four serotypes (DENV1-4). Beginning with a section on protocols to produce DENV and its proteins that are fundamental for many biophysical, biochemical, immunological, and cellular studies, the book continues by covering protocols to study the interactions between DENV and cellular proteins, DENV immunopathogenesis and diagnosis, as well as recent advances in animal models that can be used in studies of DENV immunopathogenesis and vaccine development. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Dengue Virus: Methods and Protocols serves as an ideal guide for researchers attempting to further understand this critical threat to worldwide public health.

N° de réf. du vendeur 9781071618783

Détails bibliographiques

• Titre: Dengue Virus : Methods and Protocols
• Éditeur: Springer US, Springer US
• Date de parution: 2021
• Langue: anglais
• ISBN à 10 chiffres: 1071618784
• ISBN à 13 chiffres: 9781071618783
• Reliure: Buch
• État de l'article: Neu
• Dimensions: 260 L x 183 H x 23 P (en millimètres) 260 millimètres de largeur par 183 millimètres de hauteur par 23 millimètres de profondeur
• Poids: 780 grammes

A propos de ce titre

Synopsis

Part I: Virus and Virus Protein Production

1. Virus Propagation and Plaque Assay for Dengue Virus

Pedro Henrique Carneiro, Lucas Mendes-Monteiro, and Ronaldo Mohana-Borges

2. Transmission Electron Microscopy Studies of Dengue Viruses

Gabriela Cardoso Caldas, Ortrud Monika Barth, and Debora Ferreira Barreto-Vieira

3. Production of Properly Folded NS1 Protein in Bacterial Cells

Diego Allonso

4. Expression and Purification of Dengue NS1 Protein in Sf9-Baculovirus System

Diego R. Coelho and Pedro Henrique Carneiro

5. Purification of Dengue and Zika Virus Non-Structural Protein 5 for Crystallization and Screening of Antivirals

Aicha Gharbi-Ayachi, Abbas El Sahili, and Julien Lescar

6. Obtention of Dengue Virus Membrane Proteins and Role for Virus Assembly

Szu-Chia Hsieh, Wen-Yang Tsai, and Wei-Kung Wang

7. Construction of Genomic and Sub-Genomic Dengue Infectious Replicons

Thu M. Cao and Richard J. Kuhn

Part II: Interaction between Dengue Virus and Cellular Proteins

8. Dengue Virus Capsid-Protein Dynamics in Live Infected Cells Studied by Pair Correlation Analysis

Manuela V. Gabriel, Ignacio Sallaberry, Guadalupe S. Costa Navarro, Enrico Gratton, Andrea V. Gamarnik, and Laura C. Estrada

9. Yeast Two-Hybrid System for Mapping Novel Dengue Protein Interactions

Jonas Nascimento Conde

10. Isolation and Identification of Dengue Virus Interactome with Human Plasma Proteins by Affinity Purification-Mass Spectrometry

Vivian Huerta and Yassel Ramos

Part III: Immunopathogensis and Diagnosis

11. Molecular Diagnosis Of Dengue

Priscila C.G. Nunes, Monique R.Q. Lima, and Flávia B. dos Santos

12. Serological Diagnosis of Dengue

Monique R.Q. Lima, Priscila C.G. Nunes, and Flávia B. dos Santos

13. Measuring Transendothelial Electrical Resistance (TEER) for Dengue Infection Studies

Jonas Nascimento Conde, Megan Mladinich, William Schutt, and Erich R. Mackow

14. Evaluation of DENV-Induced Endothelial Cell Permeability by Measurements of Transendothelial Electrical Resistance (TEER) and Extravasation of Proteins and Virus

Lana Monteiro Meuren, Sharton Vinicius Antunes Coelho, and Luciana Barros de Arruda

15. In Vitro Cytokine Production by Dengue-Infected Human Monocyte-Derived Dendritic Cells

Allan Henrique Depieri Cataneo, Juliano Bordignon, Pryscilla Fanini Wowk, and Guilherme Ferreira Silveira