Essential Genes and Genomes

Ren Zhang

ISBN 10: 1071617192 / ISBN 13: 9781071617199

Edité par Springer US, Springer US Okt 2021, 2021

A propos de cet article

Neuware -This book provides state-of-the-art information on gene essentiality screenings in a wide variety of organisms, i.e. screening for protein-coding genes and other genomic elements that are required by an organism to survive under specific conditions. With a focus on the two techniques that have revolutionized the field, the collection begins with chapters employing CRISPR/Cas9-based approaches followed by Tn-seq-based approaches, but later chapters also delve into other techniques for exploring essential genes, such as bioinformatics methods. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 448 pp. Englisch.

N° de réf. du vendeur 9781071617199

Détails bibliographiques

• Titre: Essential Genes and Genomes
• Éditeur: Springer US, Springer US Okt 2021
• Date de parution: 2021
• Langue: anglais
• ISBN à 10 chiffres: 1071617192
• ISBN à 13 chiffres: 9781071617199
• Reliure: Buch
• État de l'article: Neu
• Dimensions: 260 L x 183 H x 30 P (en millimètres) 260 millimètres de largeur par 183 millimètres de hauteur par 30 millimètres de profondeur
• Poids: 1034 grammes

A propos de ce titre

Synopsis

1. A Method to Map Gene Essentiality of Human Pluripotent Stem Cells by Genome-Scale CRISPR Screens with Inducible Cas9

Barbara Mair, Michael Aregger, Amy H.Y. Tong, Katherine S.K. Chan, and Jason Moffat

2. CRISPR/Cas9 Screening to Identify Conditionally Essential Genes in Human Cell Lines

Kimberly S. Huggler, Nicholas J. Rossiter, Kyle M. Flickinger, and Jason R. Cantor

3. Target Identification of Small Molecules Using Large-Scale CRISPR-Cas Mutagenesis Scanning of Essential Genes

Bert Kwanten, Jasper Neggers, and Dirk Daelemans

4. Design and Generation of a CRISPR Interference System for Genetic Repression and Essential Gene Analysis in the Fungal Pathogen Candida albicans

Lauren Wensing and Rebecca S. Shapiro

5. Identification of Essential Genes Using Sequential CRISPR and siRNA Screens

Luke DeHart, Oliver P. Yockey, and Jesse Bakke

6. A Simple Method that Combines CRISPR and AID to Quickly Generate Conditional Knockouts for Essential Genes in Various Vertebrate Cell Lines

Kohei Nishimura and Tatsuo Fukagawa

7. New Method for Genome-Scale Functional Genomics Study in Bacteria with Superior Performance: CRISPR Interference Screen

Xihao Liao, Xin-Hui Xing, and Chong Zhang

8. A CRISPR-Based Method for Constructing Conditional Mutations of Essential Genes in Cyanobacteria

Ju-Yuan Zhang, Tian-Cai Niu, Gui-Ming Lin, and Cheng-Cai Zhang

9. A Method of Transposon Insertion Sequencing in Comprehensively Identifying Vibrio vulnificus Genes Required for Growth in Human Serum

Miguel Carda-Diéguez and Carmen Amaro

10. The Use of Tn-Seq and the FiTnEss Analysis to Define the Core Essential Genome of Pseudomonas aeruginosa

Bradley E. Poulsen, Anne E. Clatworthy, and Deborah T. Hung

11. Analysis of Escherichia coli K1 Virulence Genes by Transposon-Directed Sequencing

Alex J. McCarthy and Peter W. Taylor

12. Using Genome Scale Mutant Libraries to Identify Essential Genes

Kevin S. Myers, Piyush Lal, Daniel R. Noguera, and Timothy J. Donohue

13. Identification and Analysis of Essential Genes in Streptococcus mutans with Transposon Sequencing

Alejandro R. Walker and Robert C. Shields

14. The Use of TnSeq to Identify Essential Alphaproteobacterial Genes Reveals Operational Variability in Conserved Developmental and Cell Cycle Systems

Gayatri Sharma and Patrick D. Curtis

15. A Proposed Framework to Identify Dispensable and Essential Functions in Bifidobacteria: Case Study of Bifidobacterium breve UCC2003 as a Prototype of Its Genus