Ethylene Signaling: Methods and Protocols

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ISBN 10: 1493983180 / ISBN 13: 9781493983186

Edité par Humana, 2018

2018. Paperback. . . . . . Books ship from the US and Ireland. N° de réf. du vendeur V9781493983186

Synopsis :

This volume provides a collection of protocols aimed toward the study of ethylene signaling in plants. Ethylene Signaling: Methods and Protocols is divided into three sections: ethylene biosynthesis, the signal transduction pathway, and the diverse ethylene responses of dicots and monocots. The chapters in section one discuss techniques for the measurement of activities related to the biosynthetic enzymes ACC synthase and ACC oxidase, the levels of ethylene synthesized by plants, and the treatment of plants with exogenous ethylene. Section two focuses on the analysis of the new membrane-associated proteins involved in the initial perception and transduction of the ethylene signal, such as ethylene receptors, CTR1, and EIN2. The third section covers assays applicable to dicots and monocots, including methods related to the roles of ethylene in germination, growth, abscission, abiotic stress, and defense. Section three also includes information on Arabidopsis mutantsand the variety of chemical inhibitors that affect ethylene responses. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Thorough and comprehensive, Ethylene Signaling: Methods and Protocols is a valuable resource for both experienced and beginner researchers with prior experience in the study of ethylene signaling and for those who are just entering this exciting research field.

 

Détails bibliographiques

Titre : Ethylene Signaling: Methods and Protocols
Éditeur : Humana
Date d'édition : 2018
Reliure : Couverture souple
Etat : New