Microbial Gene Essentiality: Protocols and Bioinformatics (Methods in Molecular Biology)

Andrei L. Osterman (Editor), Svetlana Y. Gerdes (Editor)

ISBN 10: 1588293785 / ISBN 13: 9781588293787

Edité par Humana Press, 2008

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N° de réf. du vendeur 1588293785--A223

Détails bibliographiques

• Titre: Microbial Gene Essentiality: Protocols and Bioinformatics (Methods in Molecular Biology)
• Éditeur: Humana Press
• Date de parution: 2008
• Langue: anglais
• ISBN à 10 chiffres: 1588293785
• ISBN à 13 chiffres: 9781588293787
• Reliure: Hardcover
• État de l'article: New
• Catalogues du vendeur:
  • LIFE SCIENCES

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Synopsis

This collection of experimental and computational strategies and techniques for microbial genome-scale essentiality studies was developed and written by the leading research groups in the field. In addition to wet-lab protocols, the book describes statistical methods essential for planning and evaluating successful large-scale essentiality screens; in-silico prediction of gene essentiality using genome-scale reconstructed metabolic models; and data integration and comparative analysis of genomic databases. Each protocol follows the successful Methods in Molecular Biology(TM) series format, offering an introduction outlining the underlying principles, step-by-step instructions, a list of necessary equipment and reagents, and tips on troubleshooting and avoiding pitfalls.