Modified PNAs: Synthesis and Interaction Studies with DNA & AuNPs : Making medicines from peptide nucleic acids (PNAs) using bio-inspired chemistry

Gourishankar Aland

ISBN 10: 3659138509 / ISBN 13: 9783659138508

Edité par LAP LAMBERT Academic Publishing, 2012

A propos de cet article

nach der Bestellung gedruckt Neuware - Printed after ordering - Peptide nucleic acids are a new class of DNA analogues in which charged sugar-phosphate backbone of DNA is replaced by a neutral and achiral polyamide backbone. The monomeric unit consists of N-(2-aminoethyl) glycine units to which nucleobases are attached through a conformationally rigid, tertiary acetamide linker group. The design and facile synthesis of sterically constrained new analogs of PNA having gem-dimethyl substitutions on glycine is presented. They show a higher binding to DNA relative to that with isosequential RNA. This may be a structural consequence of sterically rigid gem-dimethyl group, imposing a pre-organized conformation favorable for complex formation with cDNA. In this regard, gem-dimethyl functionality need to be explored more by introducing in aminoethyl segment or aminoethyl and glycine, both segments. Also, significant stabilization of DNA:PNA hybrids occurs upon complexation with gold nanoparticles. It is shown here that the non-thiolic polyanionic DNA can act as a template for organizing lysine capped cationic gold nanoparticles into linear superstructures. These types of assemblies may have potential for creating nanowires and lithographic circuits.

N° de réf. du vendeur 9783659138508

Détails bibliographiques

• Titre: Modified PNAs: Synthesis and Interaction Studies with DNA & AuNPs : Making medicines from peptide nucleic acids (PNAs) using bio-inspired chemistry
• Éditeur: LAP LAMBERT Academic Publishing
• Date de parution: 2012
• Langue: anglais
• ISBN à 10 chiffres: 3659138509
• ISBN à 13 chiffres: 9783659138508
• Reliure: Taschenbuch
• État de l'article: Neu
• Dimensions: 220 L x 150 H x 21 P (en millimètres) 220 millimètres de largeur par 150 millimètres de hauteur par 21 millimètres de profondeur
• Poids: 512 grammes

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Synopsis

Peptide nucleic acids are a new class of DNA analogues in which charged sugar-phosphate backbone of DNA is replaced by a neutral and achiral polyamide backbone. The monomeric unit consists of N-(2-aminoethyl) glycine units to which nucleobases are attached through a conformationally rigid, tertiary acetamide linker group. The design and facile synthesis of sterically constrained new analogs of PNA having gem-dimethyl substitutions on glycine is presented. They show a higher binding to DNA relative to that with isosequential RNA. This may be a structural consequence of sterically rigid gem-dimethyl group, imposing a pre-organized conformation favorable for complex formation with cDNA. In this regard, gem-dimethyl functionality need to be explored more by introducing in aminoethyl segment or aminoethyl and glycine, both segments. Also, significant stabilization of DNA:PNA hybrids occurs upon complexation with gold nanoparticles. It is shown here that the non-thiolic polyanionic DNA can act as a template for organizing lysine capped cationic gold nanoparticles into linear superstructures. These types of assemblies may have potential for creating nanowires and lithographic circuits.