Myofibroblasts : Methods and Protocols

David Lagares

ISBN 10: 1071613812 / ISBN 13: 9781071613818

Edité par Springer US, Springer US, 2021

A propos de cet article

Druck auf Anfrage Neuware - Printed after ordering - This detailed book explores the fundamentals of myofibroblast biology in tissue repair, fibrosis, and tumors as well as providing cutting-edge laboratory methods used to investigate myofibroblast functions in physiological and pathological settings in vitro and in vivo, as written by leading academic scientists. Section I of this volume focuses on fundamental methods to study myofibroblast biology and covers topics such as methods for detecting myofibroblasts and senescent myofibroblasts in cell culture and histology, single cell RNA sequencing to identify myofibroblast subsets in fibrotic tissues, and functional assays to assess TGF-beta activation, myofibroblast apoptosis, or matrix deposition and crosslinking. Section II discusses methods to investigate the mechanobiology of myofibroblasts in vitro, including the fabrication of functional hydrogels with tunable stiffness, the use of atomic force microscopy to characterize matrix and cellular stiffness, as well as molecular assays to assess fibroblast mechanotransduction pathways and durotaxis. Section III describes multiple animal models to investigate myofibroblast functions across organs in vivo as well as human organoid systems, precision tissue slices and decellularized 3D tissue scaffolds to assess myofibroblast functions in relevant human ex vivo models. Written for the highly successfulMethods in Molecular Biologyseries, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and extensive,Myofibroblasts: Methods and Protocolsis an essential collection for researchers delving into the processes and effects of these important cells.

N° de réf. du vendeur 9781071613818

Détails bibliographiques

• Titre: Myofibroblasts : Methods and Protocols
• Éditeur: Springer US, Springer US
• Date de parution: 2021
• Langue: anglais
• ISBN à 10 chiffres: 1071613812
• ISBN à 13 chiffres: 9781071613818
• Reliure: Buch
• État de l'article: Neu

A propos de ce titre

Synopsis

1. 50 Years of Myofibroblasts: How the Myofibroblast Concept Evolved

Giulio Gabbiani

Section I: Fundamental Methods to Study Myofibroblast Biology

2. Myofibroblast Functions in Tissue Repair and Fibrosis: An Introduction

Victor J. Thannickal

3. Myofibroblast Markers and Microscopy Detection Methods in Cell Culture and Histology

Fereshteh Younesi, Dong Ok Son, Joao Firmino, and Boris Hinz

4. Fibroblast and Myofibroblast Subtypes: Single Cell Sequencing

Hesham Soliman, Lin Wei Tung, and Fabio M.V. Rossi

5. Myofibroblast Adhesome Analysis by Mass Spectrometry

Christopher A. McCulloch

6. Myofibroblast TGF-β Activation Measurement In Vitro

Joanne Porte, Gisli Jenkins, and Amanda L. Tatler

7. Contraction Measurements Using Three-Dimensional Fibrillar Collagen Gel Lattices

James Griffith and William L. Berry

8. Techniques to Assess Collagen Synthesis, Deposition, and Cross-Linking In Vitro

Tamara Rosell-García and Fernando Rodriguez-Pascual

9. Methods for Studying Myofibroblast Apoptotic Pathways

Yan Zhou and David Lagares

10. Determination of Senescent Myofibroblasts in Precision-Cut Lung Slices

Tamara Cruz, Ana L. Mora, and Mauricio Rojas

11. The Scar-in-a-Jar: In Vitro Fibrosis Model for Anti-Fibrotic Drug Testing

Simon Stebler and Michael Raghunath

Section II: Myofibroblast Mechanobiology

12. Why Stress Matters: An Introduction

Daniel J. Tschumperlin

13. Soft Substrate Culture to Mechanically Control Cardiac Myofibroblast Activation

Natalie M. Landry, Sunil G. Rattan, and Ian M.C. Dixon

14. Quantitative Analysis of Myofibroblast Contraction by Traction Force Microscopy

Shuying Yang, Fernando R. Valencia, Benedikt Sabass, and Sergey V. Plotnikov

15. Nucleocytoplasmic Shuttling of the Mechanosensitive Transcription Factors MRTF and YAP/TAZ

Michael Kofler and András Kapus

16. Atomic Force Microscopy for Live-Cell and Hydrogel Measurement

Alexander Whitehead, Natalie J. Kirkland, and Adam J. Engler

17. Method for Investigating Fibroblast Durotaxis

Hossam Kadry, David Lagares, and Taslim A. Al-Hilal

18. Decellularized Extracellular Matrix (ECM) as a Model to Study Fibrotic ECM Mecha