Neutrophil Methods and Protocols, Second Edition (Volume 1124)

Langue : anglais

Edité par Springer, 2019

1627038442 / 9781627038447

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This expansive volume serves as a critical laboratory resource focusing entirely on neutrophils, which function as the human body's frontline cellular defense mechanism against infectious diseases. The book provides a thorough overview of the role these crucial white blood cells play in host defense systems and overall inflammatory responses. Readers will find meticulously detailed procedures covering the isolation of neutrophils from both human subjects and various animal models. Furthermore, the text delves deeply into advanced cellular techniques, offering guidance on the subcellular fractionation of human neutrophils and the complex analysis of in vivo transmigrated cells. It also explores cutting-edge laboratory practices, including the generation of fully mature neutrophils utilizing induced pluripotent stem cells (iPSCs) and the comprehensive analysis of specific neutrophil gene expressions. In addition to isolation and generation techniques, the volume thoroughly outlines practical methods for evaluating functional cellular responses. This includes detailed experimental designs for investigating cell priming, the production of oxidants, phagocytosis processes, overall bactericidal activity, and the intricate formation of neutrophil extracellular traps (NETs). Researchers investigating cellular movement and communication will also benefit from dedicated protocols covering neutrophil adhesion mechanisms, chemotaxis, and the pathways involved in outside-in integrin signaling. Adhering to the highly acclaimed and user-friendly format of the Methods in Molecular Biology series, every individual chapter is engineered for immediate laboratory application. Chapters consistently feature introductory overviews of the specific topic, complete and exhaustive lists of all required laboratory materials and chemical reagents, and readily reproducible, step-by-step procedural guidelines. Crucially, each section concludes with expert notes on troubleshooting, offering invaluable advice on how to bypass common experimental errors and known pitfalls. Ultimately, this authoritative and highly accessible text stands as an indispensable tool for both basic scientists and clinical researchers seeking to apply the most modern methodological advances in their ongoing work with neutrophil biology.…

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Titre
Neutrophil Methods and Protocols, Second Edition (Volume 1124)
Auteur
Mark T. Quinn, Frank R. DeLeo
Éditeur
Springer
Année de publication
2019
État de l'article
Neuf
Reliure
Couverture rigide
Langue
anglais
ISBN à 10 chiffres
1627038442
ISBN à 13 chiffres
9781627038447
Édition
2ème Édition
Poids de l'article
1,5 kilogramme

Salish Sea Books

Nanaimo, BC, Canada

Vendeur avec une évaluation de 4 étoiles

Vendeur AbeBooks depuis 23 septembre 2026

Frais d'expédition de Canada vers Etats-Unis

Article7 à 14 jours ouvrés5 à 8 jours ouvrés
Premier articleEUR 11,59EUR 33,03
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Salish Sea Books is located in Nanaimo, British Columbia, specializing in a diverse, curated selection of used books and media. As a senior, one-man show, I handle every single aspect of this business personally. Because of this, I care deeply and passionately about getting your order right and providing genuine care for my customers. My ultimate goal is to ensure you know exactly what you are buying before you click purchase, and that you receive the highest level of personal service from my shelf to your door.…

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