Oligonucleotides and Analogues: A Practical Approach (Practical Approach Series)

Eckstein, Fritz

ISBN 10: 0199632790 / ISBN 13: 9780199632794

Edité par Oxford University Press, USA, 1992

A propos de cet article

N° de réf. du vendeur 36J90_85_0199632790

Détails bibliographiques

• Titre: Oligonucleotides and Analogues: A Practical Approach (Practical Approach Series)
• Éditeur: Oxford University Press, USA
• Date de parution: 1992
• Langue: anglais
• ISBN à 10 chiffres: 0199632790
• ISBN à 13 chiffres: 9780199632794
• Reliure: Couverture souple
• État de l'article: new

A propos de ce titre

Synopsis

Interest in oligonucleotide synthesis has expanded dramatically since the publication of M. Gait's book in this series in 1984. The process has been automated, new reagents have been developed, and the range of uses for the compounds produced has increased and continues to grow. This volume provides practical guidance on oligonucleotide synthesis and methods for introducing modifications into these molecules. State- of-the-art techniques for automated synthesis are covered in two chapters on the production of oligodeoxynucleotides and oligoribonucleotides. Synthesis of modified oligodeoxynucleotides is also detailed, including modification of the phosphate backbone to produce phosphorothioates, phosphorodithiolates and methyl phosphonates, all of which are of considerable importance due to their potential therapeutic applications. Other chapters describe production of sugar-modified ilgodeoxynucleotides and the attachment of various reported groups; these techniques are useful to those interested in non-radioactive probes for hybridization and for the study of DNA-DNA and DNA protein interactions. This book will be of interest to academic and industrial researchers, enabling both chemists and non-chemists to synthesize oligonucleotides and analogues for a wide variety of experimental purposes.