PCR Mutation Detection Protocols

ISBN 10: 0896036170 / ISBN 13: 9780896036178

Edité par Humana Press, 2002

A propos de cet article

pp. x + 212 1st Edition. N° de réf. du vendeur 26290052

Détails bibliographiques

Titre : PCR Mutation Detection Protocols

Éditeur : Humana Press

Date d'édition : 2002

Reliure : Couverture souple

Etat : Used

A propos de ce titre

Synopsis :

Bimal D. Theophilus and Ralph Rapley provide biological and clinical investigators with a comprehensive collection of new, recent, and updated PCR-based screening methods suitable for detecting the presence of both known and novel mutations. The methods cover point mutations (e.g., ASO-PCR, SSCP, DGGE, chemical cleavage), deletions (multiplex PCR, FISH, blotting), non-sense mutations (PTT), and more. The new and exciting techniques of DNA array analysis, along with such recently developed experimental methods as conformation-sensitive gel electrophoresis, are also included. Each chapter explains the basic theory behind the technique and provides valuable notes essential for its successful execution.

Présentation de l'éditeur:

The Second Edition updates the subject with key protocols involving the PCR and its many incarnations such as SSCP, CSGE, and dHPLC. The book also addresses such areas as Southern blotting, accurate diagnostics with high throughput, and microarray systems.