PCR Mutation Detection Protocols

Theophilus Bimal D. M.

ISBN 10: 0896036170 / ISBN 13: 9780896036178

Edité par Humana Press, 2002

A propos de cet article

pp. x + 212.

N° de réf. du vendeur 18290062

Détails bibliographiques

• Titre: PCR Mutation Detection Protocols
• Éditeur: Humana Press
• Date de parution: 2002
• Langue: anglais
• ISBN à 10 chiffres: 0896036170
• ISBN à 13 chiffres: 9780896036178
• Reliure: Couverture souple
• État de l'article: Used

A propos de ce titre

Synopsis

Bimal D. Theophilus and Ralph Rapley provide biological and clinical investigators with a comprehensive collection of new, recent, and updated PCR-based screening methods suitable for detecting the presence of both known and novel mutations. The methods cover point mutations (e.g., ASO-PCR, SSCP, DGGE, chemical cleavage), deletions (multiplex PCR, FISH, blotting), non-sense mutations (PTT), and more. The new and exciting techniques of DNA array analysis, along with such recently developed experimental methods as conformation-sensitive gel electrophoresis, are also included. Each chapter explains the basic theory behind the technique and provides valuable notes essential for its successful execution.

Présentation de l'éditeur

The Second Edition updates the subject with key protocols involving the PCR and its many incarnations such as SSCP, CSGE, and dHPLC. The book also addresses such areas as Southern blotting, accurate diagnostics with high throughput, and microarray systems.