Plant Mitochondria : Methods and Protocols

Allan G. Rasmusson

ISBN 10: 1071616528 / ISBN 13: 9781071616529

Edité par Springer US, Springer New York, 2021

A propos de cet article

Druck auf Anfrage Neuware - Printed after ordering - This detailed volume presents a wide range of techniques for plant mitochondrial analysis, ranging from tried-and-tested work horse techniques to the latest innovations. Within its pages, it explores subjects such as affinity-based isolation of mitochondria with magnetic beads, mitochondrial quality assessment protocols, measurement of uptake and release of specific metabolites, mitochondrial protein identification and visualization, as well as gene splicing and editing, and much more. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Plant Mitochondria: Methods and Protocols provides a highly useful set of methodologies for the plant mitochondrial community to help discover more interesting aspects of plant mitochondria in the years to come. N° de réf. du vendeur 9781071616529

Détails bibliographiques

Titre : Plant Mitochondria : Methods and Protocols

Éditeur : Springer US, Springer New York

Date d'édition : 2021

Reliure : Buch

Etat : Neu

A propos de ce titre

Synopsis :

1. Purification of Leaf Mitochondria from Arabidopsis thaliana Using Percoll Density Gradients

Huy Cuong Tran and Olivier Van Aken

2. Cell Type-Specific Isolation of Mitochondria in Arabidopsis

Clément Boussardon and Olivier Keech

3. Isolation of Plant Mitochondria Using Affinity Purification

Franziska Kuhnert and Andreas P.M. Weber

4. Isolation of Highly Purified, Intact, and Functional Mitochondria from Potato Tubers Using a Two-in-One Percoll Density Gradient

Ian Max Møller and Allan G. Rasmusson

5. Integrity Assessment of Isolated Plant Mitochondria

Allan G. Rasmusson, Mengshu Hao, and Ian Max Møller

6. High-Throughput Oxygen Consumption Measurements in Leaf Tissue Using Oxygen Sensitive Fluorophores

Brendan M. O'Leary, Glenda Guek Khim Oh, and A. Harvey Millar

7. Assessment of Respiratory Enzymes in Intact Cells by Permeabilization with Alamethicin

Allan G. Rasmusson, Ian Max Møller, and Susanne Widell

8. Assessing the Kinetics of Metabolite Uptake and Utilization by Isolated Mitochondria Using Selective Reaction Monitoring-Mass Spectrometry (SRM-MS)

Xuyen Le, A. Harvey Millar, and Chun Pong Lee

9. Complexome Profiling of Plant Mitochondrial Fractions

Lucie Schröder, Holger Eubel, and Hans-Peter Braun

10. High Throughput BN-PAGE for Mitochondrial Respiratory Complexes

Lilian Vincis Pereira Sanglard and Catherine Colas des Francs-Small

11. Assessment of Mitochondrial Protein Composition and Purity by Mass Spectroscopy

Anqiang Tang, Yimin Wang, and Nicolas L. Taylor

12. Mitochondrial GFP-Tagged Protein Localization Using Transient Transformations in Arabidopsis thaliana

Cristina Ruberti

13. Assessment of Mitochondrial Protein Topology and Membrane Insertion

Kerstin Schäfer, Carina Engstler, Korbinian Dischinger, and Chris Carrie

14. Assessment of Protein Synthesis in Mitochondria Isolated from Rosette Leaves and Liquid Culture Seedlings of Arabidopsis

Malgorzata Kwasniak-Owczarek, Artur Tomal, and Hanna Janska

15. Markers for Mitochondrial ROS Status

Monika Ostaszewska-Bugajska, Anna Podgórska, and Bożena Szal

16. Mass Spectrometry-Based Quantitative Cysteine Redox Proteome Profiling of Isolated Mitochondria Using Differential iodoTMT Labeling

Jonas Giese, Jürgen Eirich, Frederik Post, Markus Schwarzländer, and Iris Finkemeier

17. Analysis of the Plant Mitochondrial Transcriptom