Post-Transcriptional Gene Regulation. Cet article n’est pas disponible.Post-Transcriptional Gene Regulation

Part I: Bioinformatics

1. Introduction to Bioinformatics Resources for Post-Transcriptional Regulation of Gene Expression

Eliana Destefanis and Erik Dassi

2. Predicting RNA Secondary Structure Using In Vitro and In Vivo Data

Riccardo Delli Ponti and Gian Gaetano Tartaglia

3. RBPmap: A Tool for Mapping and Predicting the Binding Sites of RNA-Binding Proteins Considering the Motif Environment

Inbal Paz, Amir Argoetti, Noa Cohen, Niv Even, and Yael Mandel-Gutfreund

Part II: Expression Studies

4. Analysis of mRNA Translation by Polysome Profiling

Anne Cammas, Pauline Herviou, Leïla Dumas, and Stefania Millevoi

5. Exploring Ribosome-Positioning on Translating Transcripts with Ribosome Profiling

Alexander L. Cope, Sangeevan Vellappan, John S. Favate, Kyle S. Skalenko, Srujana S. Yadavalli, and Premal Shah

6. Identification of RNA Binding Partners of CRISPR-Cas Proteins in Prokaryotes Using RIP-Seq

Sahil Sharma and Cynthia M. Sharma

7. Rapidly Characterizing CRISPR-Cas13 Nucleases Using Cell-Free Transcription-Translation Systems

Katharina G. Wandera and Chase L. Beisel

Part III: Interactomics

8. Studying RNP Composition with RIP

Annalisa Rossi and Alberto Inga

9. PAR-CLIP: A Method for Transcriptome-Wide Identification of RNA Binding Protein Interaction Sites

Charles Danan, Sudhir Manickavel, and Markus Hafner

10. A Pipeline for Analyzing eCLIP and iCLIP Data with htseq-clip and DEWSeq

Sudeep Sahadevan, Thileepan Sekaran, and Thomas Schwarz

11. Identification of miRNAs Bound to an RNA of Interest by MicroRNA Capture Affinity Technology (miR-CATCH)

Andrea Zeni, Margherita Grasso, and Michela A. Denti

12. Identifying the Protein Interactomes of Target RNAs Using HyPR-MS

Katherine B. Henke, Rachel M. Miller, Rachel A. Knoener, Mark Scalf, Michele Spiniello, and Lloyd M. Smith

Part IV: The RNA Lifecycle

13. Visualization and Quantification of Subcellular RNA Localization Using Single Molecule RNA Fluorescence In Situ Hybridization

Ankita Arora, Raeann Goering, Pedro Tirado Velez, and J. Matthew Taliaferro

14. Single-Molecule RNA Imaging Using Mango II Arrays

Adam D. Cawte, Haruki Iino, Peter J. Unrau, and David S. Rueda

15. Genome-Wide Identification of Polyadenylation Dynamics with TED-Seq

Yeonui Kwak and Hojoong Kwa