G Protein-Coupled Receptor Screening Assays: Methods and Protocols

Martins, Sofia Aires

ISBN 10: 1071612204 / ISBN 13: 9781071612200

Edité par Humana, 2021

2021. 2nd ed. 2021. hardcover. . . . . . Books ship from the US and Ireland. N° de réf. du vendeur V9781071612200

Synopsis :

1. Isolation of Lipid Rafts by the Detergent and Non-Detergent Based Methods for Localization of GPCRs with Immunoblotting and Laser Scanning Confocal Microscopy

Peter Abdelmaseeh, Andrew C. Tiu, Selim Rozyyev, Laureano D. Asico, Pedro A. Jose, Van Anthony M. Villar

2. Detection of GPCR mRNA Expression in Primary Cells via qPCR, Microarrays, and RNA-Sequencing

Krishna Sriram, Cristina Salmerón, Anna Di Nardo, and Paul A. Insel

3. Construction of Recombinant Cell Lines for GPCR Expression

Philip J. Reeves

4. Recombinant Expression and Purification of Cannabinoid Receptor CB₂, a G Protein-Coupled Receptor

Alexei A. Yeliseev

5. Screening for Serotonin Receptor 4 Agonists Using GPCR-Based Sensor in Yeast

Emily A. Yasi and Pamela Peralta-Yahya

6. Immobilization of Olfactory Receptors Carried by Nanosomes onto a Gold Sensor Surface

Jasmina Vidic and Yanxia Hou

7. Screening Methods for Cell-Free Synthesized GPCR/Nanoparticle Samples

Zoe Köck, Volker Dötsch, and Frank Bernhard

8. Fluorescence Anisotropy-Based Assay for Characterization of Ligand Binding Dynamics to GPCRs: The Case of Cy3B-Labelled Ligands Binding to MC₄ Receptors in Budded Baculoviruses

Santa Veiksina, Maris-Johanna Tahk, Tõnis Laasfeld, Reet Link, Sergei Kopanchuk, and Ago Rinken

9. Bioluminescence in G Protein-Coupled Receptors Drug Screening Using Nanoluciferase and Halo-Tag Technology

Hannes Schihada, Katarina Nemec, Martin J. Lohse, and Isabella Maiellaro

10. Nanoluciferase-Based Complementation Assay to Detect GPCR-G Protein Interaction

Céline Laschet and Julien Hanson

11. Imaging of Genetically-Encoded FRET-Based Biosensors to Detect GPCR Activity

Luca Bordes, Sergei Chavez-Abiega, and Joachim Goedhart

12. cAMP Biosensor Assay Using BacMam Expression System: Studying the Downstream Signaling of LH/hCG Receptor Activation

Darja Lavogina, Tõnis Laasfeld, Maris-Johanna Tahk, Olga Kukk, Anni Allikalt, Sergei Kopanchuk, and Ago Rinken

13. FLIPR Calcium Mobilization Assays in GPCR Drug Discovery

Grzegorz Woszczek, Elisabeth Fuerst, and Thomas J.A. Maguire

14. Live Cell Imaging and Optogenetics-Based Assays for GPCR Activity

Xenia Meshik and N. Gautam

15. Split-Tobacco Etch Virus (Split-TEV) Method in G Protein-Coupled Receptor Interacting Proteins

Marta Alonso-Gardón and Raúl Estévez

16. NanoLuc-Based Methods to Measure β-Arrestin2 Recruitment to G Protein-Coupled

Détails bibliographiques

Titre : G Protein-Coupled Receptor Screening Assays:...
Éditeur : Humana
Date d'édition : 2021
Reliure : Couverture rigide
Etat : New
Edition : 2ème Édition