Preface...
Table of Contents...
Contributing Authors...
1. Protein Stains and Applications
Pazhani Sundaram
2. The Roles of Acetic Acid and Methanol during Fixing and Staining Proteins in a SDS Polyacrylamide Electrophoresis Gel
J.P. Dean Goldring
3. Multicolored Prestained Standard Protein Marker Generation using a Variety of Remazol Dyes for Easy Visualization of Protein Bands during SDS-PAGE
Gaurav Kumar
4. Coomassie-Brilliant Blue Staining of Polyacrylamide Gels
Claudia Arndt, Stefanie Koristka, Anja Feldmann, Ralf Bergmann, and Michael Bachmann
5. A Simple, Time-Saving Dye Staining of Proteins in Sodium Dodecyl Sulfate Polyacrylamide Gel using Coomassie Blue
Wei-hua Dong, Fang Wang, Jun-he Zhang, Yan-sheng Zhou, Ling-ye Zhang, and Tian-yun Wang
6. Application of Heat to Quickly Stain and Destain Proteins Stained with Coomassie Blue
Biji T. Kurien and R. Hal Scofield
7. Silver Staining Techniques of Polyacrylamide Gels
Nicole Berndt, Rolf Bergmann, Claudia Arndt, Stefanie Koristka, and Michael Bachmann
8. Counterion Dye Staining of Proteins in One- and Two-Dimensional Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis and Tryptic Gel Digestion of Stained Protein for Mass Spectrometry
Sun-Yong Hwang and Jung-Kap Choi
9. Detection of Phosphoproteins in Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis using 8-Quinolinol Stain
Sun-Yong Hwang, Xu Wang, and Jung-Kap Choi
10. Microwave Assisted Protein Staining, De-Staining and In-Gel/In-Solution Digestion of Proteins
Jennie R. Lill and Victor J. Nesatyy
11. Fluorescent Staining of Gels
Engelbert Buxbaum
12. A Single-Step Simultaneous Protein Staining Procedure for Polyacrylamide Gels and Nitrocellulose Membranes by Alta during Western Blot Analysis
Jayanta K. Pal, Sunil K. Berwal, and Rupali N. Soni
13. TEMED Enhanced Photoluminescent Imaging of Human Serum Proteins by Quantum Dots after PAGE
Na Na and Jin Ouyang
14. Detection of Glycoproteins in Polyacrylamide Gels using Pro-Q Emerald 300 Dye, A Fluorescent Periodate Schiff-Base Stain
Padmaja Mehta-D'souza
15. Curcumin/Turmeric as an Environment-Friendly Protein Gel Stain
Biji T. Kurien, Yaser Dorri, and R. Hal Scofield
16. Detection of Multiple Enzymes in Fermentation Broth using Single PAGE Analysis
Divakar, K., Deepa Arul Priya, J, Panneer Selvam, G., Suryia Prabha, M., Ashwin Kannan, Nandhini Devi, G., and Pennathur Gautam
17. Revisit of Imidazole-Zinc Reverse Stain for Protein Polyacrylamide Gel Electrophoresis
Han-Min Chen
18. A One-Step Staining Protocol for In-Gel Fluorescent Visualization of Proteins
Jelena Bogdanovic Pristov and Ivan Spasojevic
19. Tem Minute stain to Detect Proteins in Polyacrylamide Electrophoresis Gels with Direct red 81 and Amido Black
J.P. Dean Goldring and Robert G.E. Krause
20. In-Gel Protein Phosphatase Assay using Fluorogenic Substrates
Isamu Kameshita, Noriyuki Sueyoshi, and atsuhiko Ishida
21. Detection of Proteins in Polyacrylamide Gels via Pre-Labeling by Isatoic Anhydride
Kazem Asadollahi, Saharnaz Rafiee, and Gholamhossein Riazi
22. Fluorescent Protein Visualization Immediately after Gel Electrophoresis using an In-Gel Trichloroethanol Photoreaction with Tryptophan
Carol L. Ladner-Keay, Raymond J. Turner, and Robert A. Edwards
23. Direct Immunodetection of Antigens within the Precast Polyacrylamide Gel
Surbhi Desai, Boguslawa R. Dworecki, and Marie C. Nlend
24. Zymographic Determination of Intrinsic Specific Activity
