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D�tails bibliographiques

Titre: RNA Processing: A Practical ApproachVolume II (The ^APractical Approach Series)
�diteur: Oxford University Press
Date de parution: 1994
Langue: anglais
ISBN � 10 chiffres: 019963470X
ISBN � 13 chiffres: 9780199634705
Reliure: Couverture souple
�tat de l'article: Good

A propos de ce titre

Synopsis

Post-transcriptional events are increasingly recognized as important sites of regulation of gene expression in eukaryotic cells and so RNA processing has rapidly become an area of intense research activity. These two volumes give detailed practical guidance on all the major aspects of this subject. Step-by-step practical protocols from leading laboratories are presented for studies of the termination and end processing, capping, methylation, splicing and editing of mRNA as well as studies of mRNA stability and processing of rRNA and tRNA. The reader is led through all key steps required for successful experimental investigation. This includes full desciptions of the synthesis and purification of RNA substrates for in vitro work, the characterization of specific RNA's and the isolation and analysis of ribonucleoprotein complexes.

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