Series Preface...
Preface...
Table of Contents...
Contributing Authors...
Part I Molecular Techniques
1. Production of High-Quality Antibodies for the Study of Receptors and Ion Channels
Masahiko Watanabe
2. Co-Immunoprecipitation from Brain
Xavier Morató, Dasiel O. Borroto-Escuela, Kjell Fuxe, Víctor Fernández-Dueñas, and Francisco Ciruela
3. Sub-Synaptic Membrane Fractionation
Paula M. Canas and Rodrigo A. Cunha
4. Quantitative Analysis of Cell Surface Expressed, Intracellular, and Internalized Neurotransmitter Receptor Populations in Brain Slices Using Biotinylation
Elek Molnár
5. Single Nanoparticle Tracking of Surface Ion Channels and Receptors in Brain Cells
Juan Varela, Julien Dupuis, François Maingret, and Laurent Groc
6. Radioligand Binding Detection of Receptors in Brain Membranes
Fuencisla Pilar-Cuéllar, Emilio Garro-Martínez, Elena Castro, and Álvaro Díaz
7. Recombinant Alphavirus-Mediated Expression of Ion Channels and Receptors in the Brain
Markus U. Ehrengruber and Kenneth Lundstrom
8. Time Resolved-Fluorescence Resonance Energy Transfer using Fluorescent Ligands to Study Native G Protein-Coupled Receptor Heteromerization in Brain
Víctor Fernández-Dueñas, Thierry Durroux, and Francisco Ciruela
9. Study of GPCR Homo and Heteroreceptor Complexes in Specific Neuronal Cell Populations using the In Situ Proximity Ligation Assay
Manuel Narváez, Minerva Crespo-Ramírez, Ramón Fores-Pons, Mariana Pita-Rodríguez, Francisco Ciruela, Malgorzata Filip, Sarah Beggiato, Luca Ferraro, Sergio Tanganelli, Patrizia Ambrogini, Miguel Perezde la Mora, Kjell Fuxe, and Dasiel O. Borroto-Escuela
10. Amplified Luminescent Proximity Homogeneous Assay (Alpha)-Based Technique to Detect GPCR Oligomers in Human Post-Mortem Brain
Marta Valle-León, Víctor Fernández-Dueñas, and Francisco Ciruela
Part II Neuroanatomical Techniques
11. Fluorescent In Situ Hybridization for Sensitive and Specific Labeling
Miwako Yamasaki and Masahiko Watanabe
12. Autoradiographic Visualization of G Protein-Coupled Receptors in Brain
Rebeca Vidal, Fuencisla Pilar-Cuellar, Álvaro Díaz, and Elena Castro
13. Localization of Neurotransmitter Receptor and Ion Channel Proteins in Unfixed Brains using In Situ Immunoblotting
Elek Molnár
14. Immunohistochemistry for Ion Channels and their Interacting Molecules: Tips for Improving Antibody Accessibility
Kohtarou Konno and Masahiko Watanabe
15. Localization of GFP-Tagged Proteins at the Electron Microscope
Sara Gil-Perotin, A. Cebrián-Silla, V. Herranz-Pérez, P. García-Belda, S. Gil-García, M. Fil, J.S. Lee, M.V. Nachury, and José Manuel García-Verdugo
16. Tyramide Signal Amplificación for Immunoelectron Microscopy
María José Ulloa-Navas, Patricia García-Tárraga, Susana González-Granero, Pedro Pérez-Borreda, Vicente Herranz-Pérez, and José Manuel García-Verdugo
17. Pre-Embedding Methods for the Localization of Receptors and Ion Channels
Rafael Luján
18. Post-Embedding Immunohistochemistry in the Localization of Receptors and Ion Channels
Rafael Luján and Masahiko Watanabe
19. High-Resolution Localization and Quantitation of Membrane Proteins by SDS-Digested Freeze-Fracture Replica Labeling (SDS-FRL)
Walter A. Kaufmann, David Kleindienst, Harumi Harada, and Ryuichi Shigemoto
20. Pre-Embedding Immunostaining of Brain Tissue and Three-Dimensional Imaging with FIB-SEM
Marta Turégano-López, José Rodrigo-Rodríguez, Lidia Alonso-Nanclares, Juncal González-Soriano, Javier DeFelipe, and Ángel Merchán-Pérez
21. Application of Virus Vectors for Anterograde Tract-Tracing and Single-Neuron Labeling Studies
Hiroyuki Hioki, Hisashi Nakamura, and Takahiro Furuta
22. Efficient Labeling of Neurons and Identification of P
