Recombinant Gene Expression Protocols

. Ed(s): Tuan, Rocky S.

ISBN 10: 0896033333 / ISBN 13: 9780896033337

Edité par Humana Press Inc., 1997

Offers a comprehensive collection of techniques available for expressing one or more genes of interest. This title describes the vectors used for expression in bacteria, yeast, viruses, nonmammalian higher eukaryotes, mammals, and plants, as well as in transgenic animals. Editor(s): Tuan, Rocky S. Series: Methods in Molecular Biology. Num Pages: 521 pages, biography. BIC Classification: MJCM; PSAK; PSD; PSF; PSX. Category: (P) Professional & Vocational; (UP) Postgraduate, Research & Scholarly; (UU) Undergraduate. Dimension: 235 x 155 x 29. Weight in Grams: 912. . 1997. Paperback. . . . . Books ship from the US and Ireland. N° de réf. du vendeur V9780896033337

Synopsis :

The current explosive progress in molecular biological research can be definitively traced to the development of molecular cloning technology. The ability to insert specific gene sequences into cloning vectors and their subse- quent expansion is the cornerstone of modem molecular biology. A direct practical outcome of molecular cloning technology is its application to ex- press specific recombinant genes. Currently, recombinant gene products are used in a wide spectrum of applications, including gene therapy, production of bioactive pharmaceuticals, synthesis of novel biopolymers, in agriculture and animal husbandry, and so on. A fundamental requirement for successful recombinant gene expression is the design of the cloning vector and the choice of the host organism for expression. Recombinant Gene Expression Protocols grows out of the need for a laboratory manual that provides the reader the background and rationale, as well as the practical protocols for the preparation of "expression constructs" and their introduction into appropriate host cells and/or organisms. The chap- ters in this book are grouped by their expression hosts, including E. coli, yeast, mammalian cells, nonmammalian eukaryotes such as plants, Xenopus, and insects, as well as in transgenic organisms. In-depth information is presented on the important characteristics of expression cloning vectors and the various methods for efficiently introducing expression constructs into target cells and/ or organisms. Throughout Recombinant Gene Expression Protocols, the authors have consistently striven for a balanced presentation of both background informa- tion and actual laboratory details.

Détails bibliographiques

Titre : Recombinant Gene Expression Protocols
Éditeur : Humana Press Inc.
Date d'édition : 1997
Reliure : Couverture souple
Etat : New