Serial Analysis of Gene Expression (SAGE)

. Ed(s): Nielsen, Kare Lehmann

ISBN 10: 1617377120 / ISBN 13: 9781617377129

Edité par Humana Press Inc., 2010

Editor(s): Nielsen, Kare Lehmann. Series: Methods in Molecular Biology. Num Pages: 208 pages, 42 black & white illustrations, 6 black & white tables, biography. BIC Classification: MFN; PSB; PSF. Category: (P) Professional & Vocational. Dimension: 229 x 152 x 12. Weight in Grams: 330. . 2010. Softcover reprint of hardcover 1st ed. 2008. paperback. . . . . Books ship from the US and Ireland. N° de réf. du vendeur V9781617377129

Synopsis :

Serial Analysis of Gene Expression (SAGE): Digital Gene Expression Profiling facilitates the introduction of SAGE into the laboratory, and provides a framework for interpreting and comparing data derived from SAGE experiments. Of the several methods of genetic profiling available, only SAGE measures the expression of both known and unknown genes. SAGE studies encompass 50,000 tags and can provide detailed knowledge of the 2000 most highly expressed genes in the tissue sample. The SAGE protocols presented are detailed, fully annotated, and tested, and are all written by experienced SAGE researchers from around the world. Part 1 is dedicated to experimental procedures of SAGE and related methods including aRNA LongSAGE, SuperSAGE, DeepSAGE, and GMAT. Part 2 provides methods for extraction and filtration of tags, analysis of ditag populations, and completing statistically correct comparisons of gene expression profiles.

Comparative transcriptomics enables scientists to understand the underlying genetics of biological changes such as development, disease, crop yield, and resistance. SAGE analysis is also used to obtain unknown tags, which can be used as gene-specific primers in Rapid Amplification of cDNA Ends (RACE) reactions to generate full-length transcripts for cloning and sequencing. This book will be an indispensable tool for any lab engaged in genetic profiling and comparative transcriptomics, and will help many laboratories to successfully implement tag-based sequencing methods and procedures and obtain comprehensive, useful, and interpretative data.

Présentation de l'éditeur: This book facilitates the introduction of SAGE into the laboratory and provides a framework for interpreting and comparing data derived from SAGE experiments. SAGE studies encompass 50,000 tags and can provide detailed knowledge of the 2000 most highly expressed genes in the tissue sample. The SAGE protocols presented are detailed, fully annotated, and tested, and are all written by experienced SAGE researchers from around the world.

Détails bibliographiques

Titre : Serial Analysis of Gene Expression (SAGE)
Éditeur : Humana Press Inc.
Date d'édition : 2010
Reliure : Couverture souple
Etat : New