TALENs

Ralf Kühn

ISBN 10: 1493949055 / ISBN 13: 9781493949052

Edité par Springer New York, Springer New York Aug 2016, 2016

A propos de cet article

Neuware -¿This volume provides a comprehensive collection of protocols on new technology across various model organisms. Chapters describe species-specific methods to generate new mutants the content is completed by chapters on natural TAL effectors, TAL element DNA binding principles, TALEN target site prediction, and methods for the efficient construction of TALEN coding regions. While addition chapter focus on the application of TALEN as sequence-specific nucleases and TAL based gene activators or inhibitors and the visualization of chromatin dynamics in live cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, TALENs: Methods and Protocols aims to ensure successful results in the further study of this vital field.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 288 pp. Englisch.

N° de réf. du vendeur 9781493949052

Détails bibliographiques

• Titre: TALENs
• Éditeur: Springer New York, Springer New York Aug 2016
• Date de parution: 2016
• Langue: anglais
• ISBN à 10 chiffres: 1493949055
• ISBN à 13 chiffres: 9781493949052
• Reliure: Taschenbuch
• État de l'article: Neu
• Dimensions: 254 L x 178 H x 16 P (en millimètres) 254 millimètres de largeur par 178 millimètres de hauteur par 16 millimètres de profondeur
• Poids: 547 grammes

A propos de ce titre

Synopsis

​This volume provides a comprehensive collection of protocols on new technology across various model organisms. Chapters describe species-specific methods to generate new mutants the content is completed by chapters on natural TAL effectors, TAL element DNA binding principles, TALEN target site prediction, and methods for the efficient construction of TALEN coding regions. While addition chapter focus on the application of TALEN as sequence-specific nucleases and TAL based gene activators or inhibitors and the visualization of chromatin dynamics in live cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, TALENs: Methods and Protocols aims to ensure successful results in the further study of this vital field.