1. Imaging Mass Cytometry in Immuno-oncology
Tiziana Cotechini, Oliver Jones, and Charles Colin Thomas Hindmarch
2. Analyzing the Tumor Immune Microenvironment by Flow Cytometry
François Santinon, Yoon Kow Young, Sonia Victoria del Rincon, and Koren K. Mann
3. Blood sample processing and banking for functional and molecular analyses
Paméla Thébault, Jean-François Cailhier, and Réjean Lapointe
4. Quantitatively Assessing the Respiratory Burst in Innate Immune Cells
Ava L. Sargent, Jordan A. Leedberg, Jessica E. Burress, Purva S. Dalwadi, Kevin S. O'Fallon, Erin Gaffney-Stomberg, and Peter C.W. Gaines
5. Visualizing NETosis using a Novel Neutrophil Extracellular Trap-Specific Marker
Meghan L. De Meo, Muhammad H. Shahzad, and Jonathan D. Spicer
6. In vitro methods to evaluate macrophage polarization and function in cancer
Aldo Ummarino, Clément Anfray, Akihiro Maeda, Fernando Torres Andón, and Paola Allavena
7. In vivo analysis of tumor associated macrophages in the tumor microenvironment
Clément Anfray, Aldo Ummarino, Alfonso Calvo, Paola Allavena, and Fernando Torres Andón
8. Assessing the immune regulatory role of metabolites in vitro
Sarah McPhedran, Junu Choe, Sarah MacPherson, Gabrielle Kowalchuk, Vanessa Chan, Marisa K. Kilgour, and Julian J. Lum
9. Mouse Models of Obesity To Study The Tumor Immune Microenvironment
Sarah Petrecca and Daniela F Quail
10. Mouse Models of Obesity To Study The Tumor Immune Microenvironment
Sarah Petrecca and Daniela F Quail
11. Assessing the efficacy of immune checkpoint inhibitors in pre-clinical tumor models
Celia Jacoberger-Foissac, Bertrand Allard, David Allard, and John Stagg
12. Fibroblast isolation from mammary gland tissue and syngeneic murine breast cancer models
Margarita Bartish, Julian Smith-Voudouris, and Sonia del Rincón
13. Multiscale label-free imaging of fibrillar collagen in the tumor microenvironment
Michael S. Nelson, Yuming Liu, Helen Wilson, Bin Li, Ivan M Rosado-Mendez, Jeremy D. Rogers, Walter F. Block, and Kevin W. Eliceiri
14. In situ measurement of intra-tumoral tissue rigidity
Benjamin E. Campbell, Stephanie Mok, and Christopher Moraes
15. Extracellular matrix glycation and crosslinking in mammary tumor progression
Jason J. Northey
16. Proteomic assessment of the murine mammary gland extracellular matrix
Samuel E. J. Preston, Vincent R. Richard, Sonia V. del Rincón, Christoph H. Borchers, and René P. Zahedi
17. Spheroids as a 3D Model of the Hypoxic Tumor Microenvironment
Sarah M. Kirsh, Sydney A. Pascetta, and James Uniacke
18. Tumor pH imaging using Chemical Exchange Saturation Transfer (CEST)-MRI
Dario Livio Longo, Elisa Pirotta, Riccardo Gambino, Feriel Romdhane, Antonella Carella, and Alessia Corrado
19. Profiling the epigenetic landscape of the tumor microenvironment using chromatin immunoprecipitation-sequencing
Marina Fukano, Gabriel Alzial, Raphaëlle Lambert, and Geneviève Deblois
20. Analysis of Spatial Molecular Data
Alona Levy-Jurgenson, Xavier Tekpli, Vessela N. Kristensen, and Zohar Yakhini
21. Laser-assisted single-cell labeling and capture
Nicolas Desjardins-Lecavalier, Graziana Modica, and Santiago Costantino
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