Cloning vectors are small DNA molecules which can have DNA fragments introduced in vitro using restriction enzymes and DNA ligases. Vectors: Cloning Applications is a handy laboratory manual which allows quick and easy access to the key protocols required by those working with vectors. This volume guides readers towards the best choice of method, conditions, equipment and reagents and provides them with trouble shooting tips to help if and when a technique runs into problems. The manual provides comprehensive coverage of vectors, including the commercially available ones which are difficult to trace through current literature and offers a different approach to an area which is central to many of the techniques used in molecular biology.

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The use of specialized cloning vectors, that facilitate the cloning, characterization and subsequent manipulation of DNA fragments, has revolutionized molecular biology. This manual describes the basic cloning procedures that must be mastered before DNA characterization or manipulation can be attempted. The subsequent applications covered include in vitro transcription and translation, cloning of very large DNA fragments for library construction, cloning of PCR products, and selected downstream manipulation studies. This book, along with its companion volume Vectors: Expression Systems, which deals with vectors for highly specific uses, is essential reading for the molecular biologist. The Essential Techniques Series books are designed to provide you with immediate access to the protocols you require every day. These handy pocket–sized manuals are easy to carry around, and conveniently spiral bound making them ideal for lab bench work. Written by experienced laboratory researchers, each book in the Essential Techniques Series gives up–to–date, tried and tested practical information for the life scientist. For each key technique these books:
∗ introduce the most commonly used methods,
∗ explain the advantages and disadvantages of the methods, and give advice on which procedure to use,
∗ provide easy to follow step–by–step protocols, with experimental notes and tips on where to pause, plus information on safety and suppliers.

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