Yeast Surface Display (Methods in Molecular Biology, 2491)

ISBN 10: 1071622846 / ISBN 13: 9781071622841

Edité par Humana, 2022

A propos de cet article

N° de réf. du vendeur I-9781071622841

Détails bibliographiques

• Titre: Yeast Surface Display (Methods in Molecular Biology, 2491)
• Éditeur: Humana
• Date de parution: 2022
• Langue: anglais
• ISBN à 10 chiffres: 1071622846
• ISBN à 13 chiffres: 9781071622841
• Reliure: Couverture rigide
• État de l'article: New

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Synopsis

This detailed volume explores a wide variety of applications of yeast surface display, an extensively used protein engineering technology. Beginning with detailed protocols for the construction and efficient selection/screening of yeast surface display libraries, as well as for the analysis of individual yeast-displayed protein variants, the book continues with protocols describing the selection of yeast surface display libraries for binding to mammalian cells or to extracellular matrix as well as protocols for a broad spectrum of specialized yeast surface display applications, demonstrating the versatility of this display platform. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible methodologies, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Yeast Surface Display serves as a comprehensive resource that enables the implementation of this powerful and versatile technique in virtually any molecular biology laboratory, even in the absence of any prior yeast surface display experience.