Peptide Nucleic Acids Methods and Protocols Methods in Molecular Biology

Nielsen Peter E Edits

ISBN 10: 0896039765 / ISBN 13: 9780896039766

Edité par Humana Press, Totowa New Jersey, 2002

A propos de cet article

From an academic library with the usual stamps and labels. Apart from the library evidence a decent copy. Volume 208 in the series Methods in Molecular Biology.

N° de réf. du vendeur A00019928

Détails bibliographiques

• Titre: Peptide Nucleic Acids Methods and Protocols Methods in Molecular Biology
• Éditeur: Humana Press, Totowa New Jersey
• Date de parution: 2002
• Langue: anglais
• ISBN à 10 chiffres: 0896039765
• ISBN à 13 chiffres: 9780896039766
• Reliure: Hard Cover
• Édition: First Edition.
• État de l'article: Good
• État de la jaquette: No Jacket
• Type de livre: A00019928

A propos de ce titre

Synopsis

Peptide nucleic acids (PNAs) have now existed for slightly more than ten years, with the interest in and applications of this pseudopeptide DNA mimic steadily increasing during the entire period. PNAs have rapidly attracted the attention of scientists from a diversity of fields ranging from (bio)organic and biophysical chemistry to prebiotic evolution, and from molecular biology to genetic diagnostics and drug development. Many of the applications take advantage of the unique properties of PNA--an uncharged pseudopeptide--that distinguish this DNA mimic from more traditional DNA analogs. Rather than trying to create a comprehensive collection of all published methods and protocols involving PNA--many of which have not yet been validated-- I have decided to concentrate on select protocols that are either very well established by several groups around the world, such as PCR-clamping and in situ hybridization, or on new methods that may have broader future impact. Basic methods for PNA oligomer synthesis and analyses have also been included. I am very grateful to those friends and colleagues who have enthusiastically contributed their work, discussions, and writing, and thereby made this book possible. Peter E. Nielsen v Contents Preface. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . v Contributors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ix IINTRODUCTION 1 PNA Technology Peter E. Nielsen. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3 II CHEMISTRY 2 Solid Phase Synthesisof PNA Oligomers Frederik Beck. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29 3 Synthesis of PNA-Peptide Conjugates Satish Kumar Awasthi and Peter E. Nielsen. . . . . . . . . . . . . . . . . . 43 4 Parallel Synthesis of PNA-Peptide Conjugate Libraries Satish Kumar Awasthi and Peter E. Nielsen. . . . . . . . . . . . . . . . . .