Protein Gel Detection and Imaging: Methods and Protocols (Methods in Molecular Biology)

Kurien, Biji T.; Scofield, R. Hal

ISBN 10: 1493987445 / ISBN 13: 9781493987443

Edité par Humana, 2018

This volume expands upon the collection of techniques published in Protein Electrophoresis: Methods and Protocols (2012) with more practical and reproducible methods to study protein gel detection and imaging. The chapters in this book cover topics such as coomassie-brilliant blue staining of polyacrylamide gels; silver staining techniques; microwave assisted protein staining, de-staining, and in-solution digestion of proteins; curumin and turmeric as an environment-friendly protein gel stain; in-gel protein phosphotase assay using fluorogenic substrates; destaining with fungal laccase; and radiolabeling and analysis of labeled proteins. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and practical, Protein Gel Detection and Imaging: Methods and Protocols is a valuable resource for expert and novice scientists and researchers who are interested in learning and experimenting with this field. N° de réf. du vendeur ABE-1579191422261

Synopsis :

Preface...

Table of Contents...

Contributing Authors...

1. Protein Stains and Applications

Pazhani Sundaram

2. The Roles of Acetic Acid and Methanol during Fixing and Staining Proteins in a SDS Polyacrylamide Electrophoresis Gel

J.P. Dean Goldring

3. Multicolored Prestained Standard Protein Marker Generation using a Variety of Remazol Dyes for Easy Visualization of Protein Bands during SDS-PAGE

Gaurav Kumar

4. Coomassie-Brilliant Blue Staining of Polyacrylamide Gels

Claudia Arndt, Stefanie Koristka, Anja Feldmann, Ralf Bergmann, and Michael Bachmann

5. A Simple, Time-Saving Dye Staining of Proteins in Sodium Dodecyl Sulfate Polyacrylamide Gel using Coomassie Blue

Wei-hua Dong, Fang Wang, Jun-he Zhang, Yan-sheng Zhou, Ling-ye Zhang, and Tian-yun Wang

6. Application of Heat to Quickly Stain and Destain Proteins Stained with Coomassie Blue

Biji T. Kurien and R. Hal Scofield

7. Silver Staining Techniques of Polyacrylamide Gels

Nicole Berndt, Rolf Bergmann, Claudia Arndt, Stefanie Koristka, and Michael Bachmann

8. Counterion Dye Staining of Proteins in One- and Two-Dimensional Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis and Tryptic Gel Digestion of Stained Protein for Mass Spectrometry

Sun-Yong Hwang and Jung-Kap Choi

9. Detection of Phosphoproteins in Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis using 8-Quinolinol Stain

Sun-Yong Hwang, Xu Wang, and Jung-Kap Choi

10. Microwave Assisted Protein Staining, De-Staining and In-Gel/In-Solution Digestion of Proteins

Jennie R. Lill and Victor J. Nesatyy

11. Fluorescent Staining of Gels

Engelbert Buxbaum

12. A Single-Step Simultaneous Protein Staining Procedure for Polyacrylamide Gels and Nitrocellulose Membranes by Alta during Western Blot Analysis

Jayanta K. Pal, Sunil K. Berwal, and Rupali N. Soni

13. TEMED Enhanced Photoluminescent Imaging of Human Serum Proteins by Quantum Dots after PAGE

Na Na and Jin Ouyang

14. Detection of Glycoproteins in Polyacrylamide Gels using Pro-Q Emerald 300 Dye, A Fluorescent Periodate Schiff-Base Stain

Padmaja Mehta-D'souza

15. Curcumin/Turmeric as an Environment-Friendly Protein Gel Stain

Biji T. Kurien, Yaser Dorri, and R. Hal Scofield

16. Detection of Multiple Enzymes in Fermentation Broth using Single PAGE Analysis

Divakar, K., Deepa Arul Priya, J, Panneer Selvam, G., Suryia Prabha, M., Ashwin Kannan, Nandhini Devi, G., and Pennathur Gautam

17. Revisit of Imidazole-Zinc Reverse Stain for Protein Polyacrylamide Gel Electrophoresis

Han-Min Chen

18. A One-Step Staining Protocol for In-Gel Fluorescent Visualization of Proteins

Jelena Bogdanovic Pristov and Ivan Spasojevic

19. Tem Minute stain to Detect Proteins in Polyacrylamide Electrophoresis Gels with Direct red 81 and Amido Black

J.P. Dean Goldring and Robert G.E. Krause

20. In-Gel Protein Phosphatase Assay using Fluorogenic Substrates

Isamu Kameshita, Noriyuki Sueyoshi, and atsuhiko Ishida

21. Detection of Proteins in Polyacrylamide Gels via Pre-Labeling by Isatoic Anhydride

Kazem Asadollahi, Saharnaz Rafiee, and Gholamhossein Riazi

22. Fluorescent Protein Visualization Immediately after Gel Electrophoresis using an In-Gel Trichloroethanol Photoreaction with Tryptophan

Carol L. Ladner-Keay, Raymond J. Turner, and Robert A. Edwards

23. Direct Immunodetection of Antigens within the Precast Polyacrylamide Gel

Surbhi Desai, Boguslawa R. Dworecki, and Marie C. Nlend

24. Zymographic Determination of Intrinsic Specific Activity

Détails bibliographiques

Titre : Protein Gel Detection and Imaging: Methods ...
Éditeur : Humana
Date d'édition : 2018
Reliure : Hardcover
Etat : Sehr gut
Edition : 1. Auflage