mRNA Decay

Maxime Wery

ISBN 10: 1071641751 / ISBN 13: 9781071641750

Edité par Springer US, Springer US Nov 2024, 2024

A propos de cet article

Neuware -This detailed volume explores techniques for researching the diverse and specialized mechanisms for mRNA degradation, both in the cytoplasm and the nucleus. From classical methods for studying RNA degradation at the single RNA level to the latest transcriptome-wide approaches involving long-read sequencing and metabolic labeling, this book focuses on methods for eukaryotic models, such as procedures for studying deadenylation, decapping and exoribonuclease activity, assessing RNA decay rate, characterizing RNA degradation intermediates, RNA-proteins interactions, and more. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, mRNA Decay: Methods and Protocols provides both new and experienced RNA researchers with an inspiring collection of protocols to prompt further investigation of these vital degradation pathways.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 440 pp. Englisch.

N° de réf. du vendeur 9781071641750

Détails bibliographiques

• Titre: mRNA Decay
• Éditeur: Springer US, Springer US Nov 2024
• Date de parution: 2024
• Langue: anglais
• ISBN à 10 chiffres: 1071641751
• ISBN à 13 chiffres: 9781071641750
• Reliure: Buch
• État de l'article: Neu
• Dimensions: 260 L x 183 H x 30 P (en millimètres) 260 millimètres de largeur par 183 millimètres de hauteur par 30 millimètres de profondeur
• Poids: 1017 grammes

A propos de ce titre

Synopsis

This detailed volume explores techniques for researching the diverse and specialized mechanisms for mRNA degradation, both in the cytoplasm and the nucleus. From classical methods for studying RNA degradation at the single RNA level to the latest transcriptome-wide approaches involving long-read sequencing and metabolic labeling, this book focuses on methods for eukaryotic models, such as procedures for studying deadenylation, decapping and exoribonuclease activity, assessing RNA decay rate, characterizing RNA degradation intermediates, RNA-proteins interactions, and more. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

 

Authoritative and practical, mRNA Decay: Methods and Protocols provides both new and experienced RNA researchers with an inspiring collection of protocols to prompt further investigation of these vital degradation pathways.