Mitochondrial Regulation: Methods and Protocols - Couverture rigide

Couverture rigide

ISBN 10 : 1071614320 ISBN 13 : 9781071614327

Editeur : Humana, 2021

Synopsis

1. Isolation of Mitochondria from Liver and Extraction of Total RNA and Protein: Analyses of miRNA and Protein Expression

André L. Simão, Pedro M. Borralho, Clifford J. Steer, Rui E. Castro, and Cecília M.P. Rodrigues

2. Determination of Oxidative Phosphorylation Complexes Activities

João S. Teodoro, Ivo F. Machado, Carlos M. Palmeira, and Anabela P. Rolo

3. BN-PAGE-Based Approach to Study Thyroid Hormones and Mitochondrial Function

Elena Silvestri, Assunta Lombardi, Federica Cioffi, and Fernando Goglia

4. Monitoring Mitochondrial Function in Mouse Embryonic Stem Cells (mESCs)

Bibiana Correia, Maria Inês Sousa, Ana F. Branco, and João Ramalho-Santos

5. Mitochondrial Functional Assessment in Mammalian Gametes Using Fluorescent Probes

Renata S. Tavares, Sara-Escada-Rebelo, Maria M. Soares, Andreia F. Silva, Teresa Almeida-Santos, Sandra Amaral, Ana Paula Sousa, and João Ramalho-Santos

6. Update on the Histoenzymatic Methods for Visualization of the Activity of Individual Mitochondrial Respiratory Chain Complexes in the Human Frozen Sections

Mariusz R. Wieckowski, Maciej Pronicki, and Agnieszka Karkucinska-Wieckowska

7. An Update on Isolation of Functional Mitochondria from Cell for Bioenergetics Studies

Magdalena Lebiedzinska-Arciszewska, Lech Wojtczak, and Mariusz R. Wieckowski

8. PCR-Based Determination of Mitochondrial DNA Copy Number in Multiple Species

Tess C. Leuthner, Jessica H. Hartman, Ian Ryde, and Joel N. Meyer

9. Methods to Monitor Mitophagy and Mitochondrial Quality: Implications in Cancer, Neurodegeneration, and Cardiovascular Diseases

Simone Patergnani, Massimo Bonora, Esmaa Bouhamida, Alberto Danese, Saverio Marchi, Giampaolo Morciano, Maurizio Previati, Gaia Pedriali, Alessandro Rimessi, Gabriele Anania, Carlotta Giorgi, and Paolo Pinton

10. Analysis of Pro-Apoptotic Protein Trafficking to and from Mitochondria

Ignacio Vega-Naredo, Gabriela Oliveira, Teresa Cunha-Oliveira, Teresa Serafim, Vilma A. Sardao, and Paulo J. Oliveira

11. Exploring Liver Mitochondrial Function by ¹³C-Stable Isotope Breath Tests: Implications in Clinical Biochemistry

Emilio Molina-Molina, Harshitha Shanmugam, Domenica Di Palo, Ignazio Grattagliano, and Piero Portincasa

12. Protocols for Mitochondria as the Target of Pharmacological Therapy in the Context of Nonalcoholic Fatty Liver Disease (NAFLD)

Ignazio Grattagliano, Agostino Di Ciaula, Jacek Baj, Emilio Molina-Molina, Harshitha Shanmugam, Gabriella Garruti, David Q.-H. Wang, and Piero Portincasa

13. The Use of Reactive Oxygen Species Production by Succinate-Driven Reverse Electron Flow as an Index of Complex 1 Activity in Isolated Brown Adipose Tissue Mitochondria

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Détails de l'édition

• Éditeur: Humana
• Date d'édition: 2021
• Langue: anglais
• ISBN 10: 1071614320
• ISBN 13: 9781071614327
• Reliure: Relié
• Numéro d'édition: 2
• Nombre de pages: 330
• Éditeur: Palmeira Carlos M., Rolo Anabela P.
• Coordonnées du fabricant: ProductSafety@springernature.com; ProductSafety@springernature.com; ; Poststr. 9; Darmstadt; 64293; Allemagne