springer us jun 2021 (29 résultats)

Buch. Etat : Neu. Neuware -This second edition offers 88 chapters divided among three volumes providing the most comprehensive source of know-how in the wide-ranging field of Mitochondrial Medicine. Volume II guides readers through chapters on mitochondrial dysfunction, functional' mitochondria, mitochondrial retrograde, mitochondrial dNTP pool quantification, mitochondrial ADP-ribosylation, blue-native gel approach, 3D optical cryo-imaging method, mitochondrial ATP and ROS production, protocol for untargeted metabolomic analysis, and methods for analysis of nitrotyrosine-containing proteins. Written in the highly successfulMethods in Molecular Biologyseries format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls.Authoritative and accessible,Mitochondrial Medicine, Second Edition, Volume 2: Assessing Mitochondria aims to be a comprehensive source of know-how in the wide-ranging field of Mitochondrial Medicine. 480 pp. Englisch.

Taschenbuch. Etat : Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This manual provides insights into the theory and practical aspects of several biotechnological and biochemical techniques for plants, protozoa, nematodes, insects and fishes, as well as human samples. The book also covers bioinformatics tools. The manual is an inclusive compilation, explaining techniques for microbial cultures, their diagnostics, DNA barcoding, microscopic techniques, blood analysis, parasite diagnostics through copro-antigens, enzyme analysis with enzyme kinetics, gene expression analysis, in-vivo protein visualization in live animals, geno-toxicity assays, quantification of micro RNAs and LncRNAs in tissue sections, the use of droplet PCR, and in-silico analysis. It provides step-by-step descriptions and details of each methodology, together with the final outcomes and inferences, in a simple and lucid manner, easily reproducible even for beginners. The broad range of techniques covered makes this volume extremely useful in understanding the principles of biotechniques, and simple applications for practical courses. 264 pp. Englisch.

Taschenbuch. Etat : Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume aims at providing a complete and updated overview of gene downregulation in plants performed through virus-induced gene silencing (VIGS). Chapters guide readers through classical and newly developed protocols of VIGS to allow readers to initiate or optimize their own silencing experiments according to the methods. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge,Virus-Induced Gene Silencing in Plants: Methods and Protocols aims to ensure successful results in the further study of this vital field. 276 pp. Englisch.

Taschenbuch. Etat : Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume provides methods and protocols on dynamin superfamily GTPases. Chapters are divided into three parts; detailing expression, purification, initial biochemical steps, undescribed analytical techniques, and biophysical and cellular characterization of DSPs in membrane remodeling, fission and fusion. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Dynamin Superfamily GTPases: Methods and Protocols to ensure successful results in the further study of this vital field. 236 pp. Englisch.

Taschenbuch. Etat : Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume explores techniques used to study either the structure or the functions of caveolae and their components in several normal and pathophysiological situations. The chapters in this book cover topics such as selective visualization of caveolae by electron microscopy techniques; spatiotemporal analysis of caveolae dynamics and mechanics using live cell fluorescence microscopy; in vitro reconstitution such as liposomes and GPMVs as tools to study caveolin- and cavin-interacting partners; pulling of tethers from the cell plasma membrane using optical tweezers; and immunofluorescence-based analysis of caveolin-3 in the diagnostic management of neuromuscular diseases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and comprehensive, Caveolae: Methods and Protocols is a valuable resource for both novice and expert researchers who are interested in discovering new roles or regulations of formed caveolae, and proteins composing their coat in various model organisms. 232 pp. Englisch.

Taschenbuch. Etat : Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume focuses on protein analysis, and covers a wide array of uses of protein microarray for disease analysis. The chapters in this book discuss different stages of protein microarrays from their construction to their use, including different types of protein microarrays such as recombinant proteins, antibody, phage, and NAPPA protein microarrays, in planar format or in solution via beads arrays. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Comprehensive and cutting-edge, Protein Microarrays for Disease Analysis: Methods and Protocols is a valuable resource for graduate and post-doctoral fellows interested in protein microarrays, as well as senior researchers interested in gaining more insight into this developing field. 296 pp. Englisch.

Taschenbuch. Etat : Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This detailed book provides an accessible compendium of up-to-date methods in the fields of environmental toxicology, molecular toxicology, and toxicogenomics. Organized into four major sections, the volume examines methods utilizing model animal species, such as nematode, fruit fly, mice, chicken, and amphibians, methods using plants to study chemical toxicity, applying the Ames assay to chemical mutagenicity study, as well as methods for environmental chemical analysis. Although this book is divided into these parts, the methods can be used across species. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Environmental Toxicology and Toxicogenomics: Principles, Methods, and Applications serves as a valuable resource for the scientific community, particularly for young scientists and graduate and undergraduate students, inspiring more research in the vitally important field of environmental toxicity, molecular toxicology, and toxicogenomics. 380 pp. Englisch.

Taschenbuch. Etat : Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This second edition offers 88 chapters divided among three volumes providing the most comprehensive source of know-how in the wide-ranging field of Mitochondrial Medicine. Volume III guides readers through chapters on intercellular mitochondrial transfer, Cytoplasmic transfer methods, Ndufs4 knockout mouse, in vivo assessment of mitochondrial oxygen consumption, mitochondrial network analysis by imaging, mitochondrial respiration in platelets, Heterologous Inferential Analysis (HIA), bioenergetic profiling, integrative methods for studying cardiac energetics, and isolation and deep sequencing of mitochondrial DNA.Written in the highly successfulMethods in Molecular Biologyseries format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls.Authoritative and accessible,Mitochondrial Medicine, Second Edition, Volume 3: Manipulating Mitochondria and Disease- Specific Approaches aims to be a comprehensive source of know-how in the wide-ranging field of Mitochondrial Medicine. 488 pp. Englisch.

Taschenbuch. Etat : Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This thorough book explores some of the most important methods and concepts affecting the quantitative analysis of the transport, targeting, and disposition of chemicals within cells, which in turn impact the macroscopic pharmacokinetics of chemical agents in the whole organism. The first half of the volume focuses on small organic molecules with drug-like characteristics, while the second half delves into the cellular pharmacokinetics of biologics and other macromolecules, including peptide therapeutics, cyclotides, antibodies, as well as nanoparticles, thus creating a comprehensive treatise that approaches cellular pharmacokinetics from the different perspectives of pharmaceutical scientists, chemical biologists, medicinal chemists, and protein engineers dealing with very different chemical agents spanning a wide range of sizes, physicochemical properties, and targeting mechanisms. Written for theMethods in Pharmacology and Toxicologyseries, chapters provide the kind of key detail and expert implementation advice that leads to excellent results in the lab. Synthetic biologists, biophysicists, and bioengineers are amongst the long list of scientists who could benefit from reading this book or from using it as a textbook.Authoritative and practical,Quantitative Analysis of Cellular Drug Transport, Disposition, and Deliverybuilds on a long history of drug development and the adding of quantitative methods at the cellular scale in order to inspire new approaches to drug development that are better able to take advantage of phenomena such as soluble-to-insoluble phase transitions or bispecific targeting, which could ultimately be exploited for the development of more effective drug delivery systems and therapeutic agents. 472 pp. Englisch.

Taschenbuch. Etat : Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This fully updated edition explores the different pathways that converge into the regulation of mitochondrial function. The book integrates mitochondria with other cellular components, discussing the dynamic properties of mitochondria with an emphasis on how these processes respond to signaling events and how they affect cellular metabolism. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and up-to-date, Mitochondrial Regulation: Methods and Protocols, Second Edition is an ideal guide for advanced undergraduates, graduates, postgraduates, and beginning researchers in the areas of molecular and cellular biology, biochemistry, and bioenergetics. 332 pp. Englisch.

Taschenbuch. Etat : Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume details different laboratory techniques used to develop regenerative therapies that help treat corneal blindness. Chapters guide readers through methods and protocols on optimal cell culture conditions, gene-editing techniques, multiple types of scaffold for corneal regeneration, how to evaluate the success of these therapies, and cell and material characterization techniques. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Corneal Regeneration: Methods and Protocols aims to be useful for new and experienced laboratory researchers working on different aspects of corneal regeneration. 264 pp. Englisch.

Taschenbuch. Etat : Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume discusses the latest experimental and clinical approaches to study seizures and epilepsy. Chapters in this book cover detailed protocols for ex vivo and in vitro experimental models and widely-used in vivo (acute and chronic seizure) models in epilepsy research. Chapters also cover techniques to screen the effective leads for the treatment of refractory seizures of pharmacoresistant epilepsy and invertebrate, non-mammalian models. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory.Cutting-edge and practical, Experimental and Translational Methods to Screen Drugs Effective Against Seizures and Epilepsy is a valuable resource for researchers, in both industry and academia, interested in learning about this important and developing field. 264 pp. Englisch.

Taschenbuch. Etat : Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This book provides a timely overview of analytical tools and methodological approaches for studying membrane lipids. It outlines the ground-breaking advances that have been made over the last two decades in high-throughput lipidomics, and in studying lipid-protein interactions, signalling pathways and the regulation of lipid metabolism.This user-friendly laboratory handbook is an ideal companion for membrane biologists, researchers, students, and clinicians alike. It is also well suited for teaching biochemistry, microbiology and biotechnology courses, making it a must-have for everyone whose work involves lipid research. 232 pp. Englisch.

Taschenbuch. Etat : Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume explores a collection of experimental techniques used to investigate different aspects of pollen development and function, including its role in reaching the ovule and delivering the two sperm cells. The techniques discussed range from basic methodology to cultivate pollen in vitro to the sophisticated experiments involving micromanipulation, Lab-on-Chip technology, or high-end imaging. The chapters in this book cover topics such as pollen grain counting using a cell counter; restricted pollination for tracing individual pollen tubes in a pistil; obtaining mutant pollen for phenotypic analysis and pollen tube dual-staining; analyzing intracellular gradients in pollen tubes; and measuring exocytosis rate in Arabidopsis pollen tube using corrected fluorescence recovery after photoconversion (cFRAPc) technique. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and thorough, Pollen and Pollen Tube Biology: Methods and Protocols is a valuable resource for novice and expert scientists interested in learning more about the field of plant reproduction. 348 pp. Englisch.

Buch. Etat : Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume brings together classical and cutting-edge protocols on the spatio-temporal study of the cellular subsets constituting the bone and the marrow in both mouse and human. Chapters details methods on bone marrow (BM) ecosystem, to label, sort, analyse, and culture specific cell subsets as well as techniques allowing the evaluation of the function of some of the cellular elements of the BM. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge,Bone Marrow Environment: Methods and Protocols aims to helpnew investigators to pursue the characterization of the BM microenvironment in the coming years. 352 pp. Englisch.

Taschenbuch. Etat : Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This second edition offers 88 chapters divided among three volumes providing the most comprehensive source of know-how in the wide-ranging field of Mitochondrial Medicine. Volume I guides readers through chapters on QSAR models, DQAsomes, synthesis of Triphenylphosphonium Phospholipid Conjugates, testing of novel isomeric mitochondriotropic derivatives, mTRIP, mitochondria-targeted imaging nanoplatforms, live-cell assessment, mitochondrial coenzyme Q10, rat liver Mitochondrial Lipidome, and Mito-SinCe2. Written in the highly successfulMethods in Molecular Biologyseries format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls.Authoritative and accessible,Mitochondrial Medicine, Second Edition, Volume 1: Targeting Mitochondria aims to be a comprehensive source of know-how in the wide-ranging field of Mitochondrial Medicine. 476 pp. Englisch.

Taschenbuch. Etat : Neu. This item is printed on demand - Print on Demand Titel. Neuware -This book provides a timely overview of analytical tools and methodological approaches for studying membrane lipids. It outlines the ground-breaking advances that have been made over the last two decades in high-throughput lipidomics, and in studying lipid-protein interactions, signalling pathways and the regulation of lipid metabolism.This user-friendly laboratory handbook is an ideal companion for membrane biologists, researchers, students, and clinicians alike. It is also well suited for teaching biochemistry, microbiology and biotechnology courses, making it a must-have for everyone whose work involves lipid research.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 232 pp. Englisch.

Buch. Etat : Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume presents techniques needed for the study oflong non-coding RNAs(lncRNAs) in cancer from their identification to functional characterization. Chapters guide readers through identification of lncRNA expression signatures in cancer tissue or liquid biopsies by RNAseq, single Cell RNAseq, Phospho RNAseq or Nanopore Sequencing techniques; validation of lncRNA signatures by Real time PCR, digital PCR or in situ hybridization; and functional analysis by siRNA or CRISPR based methods for lncRNA silencing or overexpression. Lipid based nanoparticles for delivery of siRNAs in vivo, lncRNA-protein interactions, viral lncRNAs and circRNAs are also treated in this volume. Written in the format of the highly successfulMethods in Molecular Biologyseries, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.Authoritative and practical,Long Non-Coding RNAs in Canceraims to providea collection of laboratory protocols, bioinformatic pipelines, and review chapters to further research in this vital field. 404 pp. Englisch.

Buch. Etat : Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This second edition offers 88 chapters divided among three volumes providing the most comprehensive source of know-how in the wide-ranging field of Mitochondrial Medicine. Volume III guides readers through chapters on intercellular mitochondrial transfer, Cytoplasmic transfer methods, Ndufs4 knockout mouse, in vivo assessment of mitochondrial oxygen consumption, mitochondrial network analysis by imaging, mitochondrial respiration in platelets, Heterologous Inferential Analysis (HIA), bioenergetic profiling, integrative methods for studying cardiac energetics, and isolation and deep sequencing of mitochondrial DNA.Written in the highly successfulMethods in Molecular Biologyseries format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls.Authoritative and accessible,Mitochondrial Medicine, Second Edition, Volume 3: Manipulating Mitochondria and Disease- Specific Approaches aims to be a comprehensive source of know-how in the wide-ranging field of Mitochondrial Medicine. 488 pp. Englisch.

Buch. Etat : Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This fully updated edition targets not only those assays directly involved in the discovery of GPCR-active compounds but also those involved in cell-based experiments designed to study physiological responses. Whether coming from academia or industry, or being an experienced researcher or a newcomer to the field, the reader will find accessible methods and protocols that cover the latest developments on receptor purification, molecular biology, recombinant engineering, and analytical techniques that enable the real time monitoring of the complex GPCR signaling cascade and identification of potential drug targets. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and up-to-date, G Protein-Coupled Receptor Screening Assays: Methods and Protocols, Second Edition aims to provide the tools necessary to contribute to the advancement of GPCR research and discovery and ultimately lead to the availability of innovative and more efficient drugs. 348 pp. Englisch.

Buch. Etat : Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This detailed book provides an accessible compendium of up-to-date methods in the fields of environmental toxicology, molecular toxicology, and toxicogenomics. Organized into four major sections, the volume examines methods utilizing model animal species, such as nematode, fruit fly, mice, chicken, and amphibians, methods using plants to study chemical toxicity, applying the Ames assay to chemical mutagenicity study, as well as methods for environmental chemical analysis. Although this book is divided into these parts, the methods can be used across species. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Environmental Toxicology and Toxicogenomics: Principles, Methods, and Applications serves as a valuable resource for the scientific community, particularly for young scientists and graduate and undergraduate students, inspiring more research in the vitally important field of environmental toxicity, molecular toxicology, and toxicogenomics. 380 pp. Englisch.

Buch. Etat : Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This thorough book explores some of the most important methods and concepts affecting the quantitative analysis of the transport, targeting, and disposition of chemicals within cells, which in turn impact the macroscopic pharmacokinetics of chemical agents in the whole organism. The first half of the volume focuses on small organic molecules with drug-like characteristics, while the second half delves into the cellular pharmacokinetics of biologics and other macromolecules, including peptide therapeutics, cyclotides, antibodies, as well as nanoparticles, thus creating a comprehensive treatise that approaches cellular pharmacokinetics from the different perspectives of pharmaceutical scientists, chemical biologists, medicinal chemists, and protein engineers dealing with very different chemical agents spanning a wide range of sizes, physicochemical properties, and targeting mechanisms. Written for theMethods in Pharmacology and Toxicologyseries, chapters provide the kind of key detail and expert implementation advice that leads to excellent results in the lab. Synthetic biologists, biophysicists, and bioengineers are amongst the long list of scientists who could benefit from reading this book or from using it as a textbook.Authoritative and practical,Quantitative Analysis of Cellular Drug Transport, Disposition, and Deliverybuilds on a long history of drug development and the adding of quantitative methods at the cellular scale in order to inspire new approaches to drug development that are better able to take advantage of phenomena such as soluble-to-insoluble phase transitions or bispecific targeting, which could ultimately be exploited for the development of more effective drug delivery systems and therapeutic agents. 472 pp. Englisch.

Buch. Etat : Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume focuses on protein analysis, and covers a wide array of uses of protein microarray for disease analysis. The chapters in this book discuss different stages of protein microarrays from their construction to their use, including different types of protein microarrays such as recombinant proteins, antibody, phage, and NAPPA protein microarrays, in planar format or in solution via beads arrays. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Comprehensive and cutting-edge, Protein Microarrays for Disease Analysis: Methods and Protocols is a valuable resource for graduate and post-doctoral fellows interested in protein microarrays, as well as senior researchers interested in gaining more insight into this developing field. 296 pp. Englisch.

Buch. Etat : Neu. This item is printed on demand - it takes 3-4 days longer - Neuware 356 pp. Englisch.

Buch. Etat : Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume discusses the latest experimental and clinical approaches to study seizures and epilepsy. Chapters in this book cover detailed protocols for ex vivo and in vitro experimental models and widely-used in vivo (acute and chronic seizure) models in epilepsy research. Chapters also cover techniques to screen the effective leads for the treatment of refractory seizures of pharmacoresistant epilepsy and invertebrate, non-mammalian models. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory.Cutting-edge and practical, Experimental and Translational Methods to Screen Drugs Effective Against Seizures and Epilepsy is a valuable resource for researchers, in both industry and academia, interested in learning about this important and developing field. 264 pp. Englisch.

Buch. Etat : Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This second edition offers 88 chapters divided among three volumes providing the most comprehensive source of know-how in the wide-ranging field of Mitochondrial Medicine. Volume I guides readers through chapters on QSAR models, DQAsomes, synthesis of Triphenylphosphonium Phospholipid Conjugates, testing of novel isomeric mitochondriotropic derivatives, mTRIP, mitochondria-targeted imaging nanoplatforms, live-cell assessment, mitochondrial coenzyme Q10, rat liver Mitochondrial Lipidome, and Mito-SinCe2. Written in the highly successfulMethods in Molecular Biologyseries format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls.Authoritative and accessible,Mitochondrial Medicine, Second Edition, Volume 1: Targeting Mitochondria aims to be a comprehensive source of know-how in the wide-ranging field of Mitochondrial Medicine. 476 pp. Englisch.

Buch. Etat : Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This fully updated edition explores the different pathways that converge into the regulation of mitochondrial function. The book integrates mitochondria with other cellular components, discussing the dynamic properties of mitochondria with an emphasis on how these processes respond to signaling events and how they affect cellular metabolism. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and up-to-date, Mitochondrial Regulation: Methods and Protocols, Second Edition is an ideal guide for advanced undergraduates, graduates, postgraduates, and beginning researchers in the areas of molecular and cellular biology, biochemistry, and bioenergetics. 332 pp. Englisch.

Buch. Etat : Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This second edition volume expands on the previous edition with discussions of recently developed techniques that use RNA scaffolds as molecular tools. These latest methods are used in the fields of molecular biology, cellular biology, nanotechnology, and structural biology. The chapters in this book cover topics such as RNA footprinting using small chemical reagents; using tRNA scaffolds to assist RNA crystallization; identification of RNA-binding proteins associated to RNA structural elements; and synthetic biology medicine and bacteria-based cancer therapeutics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and comprehensive, RNA Scaffolds: Methods and Protocols, Second Edition is a valuable resource that explores the fascinating uses of RNA in these different areas, and will inspire experts and novice researchers to further study this developing field. 304 pp. Englisch.

Buch. Etat : Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This second edition explores techniques involving pseudogenic DNA, RNA, and proteins. Chapters describe how to identify pseudogenes and how to study the functions and alterations of pseudogenic RNA and proteins, both in vitro, and in vivo. Written in the highly successful Methods in Molecular Biology series format, methodological chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting while avoiding known pitfalls.Authoritative and cutting-edge, Pseudogenes: Functions and Protocols, Second Edition aims to be a practical guide to researches, useful to help further their study in this field. 400 pp. Englisch.