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Synopsis
With the completion of sequencing projects and the advancement of a- lytical tools for protein identification, proteomics—the study of the expressed part of the genome—has become a major region of the burgeoning field of functional genomics. High-resolution 2-D gels can reveal virtually all p- teins present in a cell or tissue at any given time, including posttranslationally modified proteins. Changes in the expression and structure of most cellular proteins caused by differentiation or external stimuli can be displayed and eventually identified using 2-D protein gels. 2-D Proteome Analysis Protocols covers all aspects of the use of 2-D protein electrophoresis for the analysis of biological problems. The contri- tors include many of the leaders in the fields of biochemistry and analytical chemistry who were instrumental in the development of high-resolution 2-D gels, immobilized pH gradients, computer analysis, and mass spectromet- based protein identification methodologies. This book is intended as a benchtop manual and guide both for novices to 2-D gels and for those aficionados who wish to try the newer techniques. Any group using protein biochemistry—especially in the fields of molecular biology, biochemistry, microbiology, and cell biology—should find this book eminently useful. 2-D Proteome Analysis Protocols takes the researcher through the c- plete process of working with 2-D protein gels from making the protein - tract to finally identifying the proteins of interest. It includes protocols for generating 2-D protein extracts from most of the standard model organisms, including bacteria, yeast, nematode, Drosophila, plants, mouse, and human.
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2-D Proteome Analysis Protocols (Methods in Molecular Biology)
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2-d Proteome Analysis Protocols
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2-D Proteome Analysis Protocols (Paperback)
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Humana Press Inc., Totowa, NJ, 2010
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ISBN 13 : 9781617370601
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Paperback. Etat : new. Paperback. A step-by-step tour through the complete process of doing proteomics. With easy-to-follow instructions, complete with many helpful hints and explanations, leading investigators and pioneers in the field show how to make protein extracts, reproducibly run them on 2-D gels, detect them, analyze the data, and precisely identify each protein. The book covers the latest methods of using carrier ampholytes in the 1st dimension, casting and running immobilized pH gradient 2-D gels, MALDI-TOF-based peptide mapping, automated tandem mass spectrometry, and nanoelectrospray ionization technology. For the 2nd dimension, there are methods for running flatbed or vertical gels and for protein detection using autoradiography, and Coomassie, silver, and reversible metal-chelate stains. 2-D Proteome Analysis Protocols is the most complete guide for using proteomics to answer biological questions. 2-D Proteome Analysis Protocols takes the researcher through the c- plete process of working with 2-D protein gels from making the protein - tract to finally identifying the proteins of interest. Shipping may be from multiple locations in the US or from the UK, depending on stock availability. N° de réf. du vendeur 9781617370601
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2-D Proteome Analysis Protocols (Methods in Molecular Biology)
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2-D Proteome Analysis Protocols
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SPRINGER NATURE Nov 2010, 2010
ISBN 10 : 1617370606
ISBN 13 : 9781617370601
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Taschenbuch. Etat : Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -With the completion of sequencing projects and the advancement of a- lytical tools for protein identification, proteomics-the study of the expressed part of the genome-has become a major region of the burgeoning field of functional genomics. High-resolution 2-D gels can reveal virtually all p- teins present in a cell or tissue at any given time, including posttranslationally modified proteins. Changes in the expression and structure of most cellular proteins caused by differentiation or external stimuli can be displayed and eventually identified using 2-D protein gels. 2-D Proteome Analysis Protocols covers all aspects of the use of 2-D protein electrophoresis for the analysis of biological problems. The contri- tors include many of the leaders in the fields of biochemistry and analytical chemistry who were instrumental in the development of high-resolution 2-D gels, immobilized pH gradients, computer analysis, and mass spectromet- based protein identification methodologies. This book is intended as a benchtop manual and guide both for novices to 2-D gels and for those aficionados who wish to try the newer techniques. Any group using protein biochemistry-especially in the fields of molecular biology, biochemistry, microbiology, and cell biology-should find this book eminently useful. 2-D Proteome Analysis Protocols takes the researcher through the c- plete process of working with 2-D protein gels from making the protein - tract to finally identifying the proteins of interest. It includes protocols for generating 2-D protein extracts from most of the standard model organisms, including bacteria, yeast, nematode, Drosophila, plants, mouse, and human. 601 pp. Englisch. N° de réf. du vendeur 9781617370601
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2-d Proteome Analysis Protocols
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Humana, 2010
ISBN 10 : 1617370606
ISBN 13 : 9781617370601
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2-D Proteome Analysis Protocols
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2-d Proteome Analysis Protocols
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2-D Proteome Analysis Protocols (Methods in Molecular Biology)
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2-d Proteome Analysis Protocols
Edité par
Humana, 2010
ISBN 10 : 1617370606
ISBN 13 : 9781617370601
Ancien ou d'occasion
Couverture souple
Vendeur : GreatBookPricesUK, Woodford Green, Royaume-Uni
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