Articles liés à Plasmid Segregational Instability in Recombinant E.coli...

Plasmid Segregational Instability in Recombinant E.coli Fermentations: Modeling aspects of Macromixing and Plasmid loss probability in Batch and Continuous E.coli Fermentations - Couverture souple

Rama Krishna, G.V.S.

 
9783659832321: Plasmid Segregational Instability in Recombinant E.coli Fermentations: Modeling aspects of Macromixing and Plasmid loss probability in Batch and Continuous E.coli Fermentations
Couverture souple
ISBN 10 : 3659832324 ISBN 13 : 9783659832321
Editeur : LAP LAMBERT Academic Publishing, 2016

Synopsis

Segregational instability of plasmids in genetically altered Escherichia coli bacteria is not suitable for successful production of recombinant products. In this study, Escherichia coli C600 galK containing the plasmid pBR Eco gap, which codes for glyceraldehyde-3-phosphate dehydrogenase (GAPDH), was studied under industrial conditions for GAPDH production. Detailed mathematical models were developed for batch and continuous fermentations by introducing non-ideality through imperfect mixing, variable plasmid loss probability, and simulated for both fermenters by regulating mixing intensities (as a function of dilution rates). Effect of finite macromixing and a variable plasmid loss probability on plasmid stability, substrate utilization and product formation were explored by inserting generalized plasmid replication kinetics into an experimentally validated macromixing model.

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Présentation de l'éditeur

Segregational instability of plasmids in genetically altered Escherichia coli bacteria is not suitable for successful production of recombinant products. In this study, Escherichia coli C600 galK containing the plasmid pBR Eco gap, which codes for glyceraldehyde-3-phosphate dehydrogenase (GAPDH), was studied under industrial conditions for GAPDH production. Detailed mathematical models were developed for batch and continuous fermentations by introducing non-ideality through imperfect mixing, variable plasmid loss probability, and simulated for both fermenters by regulating mixing intensities (as a function of dilution rates). Effect of finite macromixing and a variable plasmid loss probability on plasmid stability, substrate utilization and product formation were explored by inserting generalized plasmid replication kinetics into an experimentally validated macromixing model.

Biographie de l'auteur

G.V.S.Rama Krishna currently works as Professor (Assistant), Biotechnology in K.L.University. He holds B.Tech.(Chemical Engg.) from N.I.T,Warangal and M.Tech.(Biotechnology) from Anna University, Chennai. Prof.G.V.S.Rama Krishna has enriched lives of Biotechnology students through academics and research for about 12 years in AICTE Institutions.

Les informations fournies dans la section « A propos du livre » peuvent faire référence à une autre édition de ce titre.

Éditeur
LAP LAMBERT Academic Publishing
Date d'édition
2016
Langue
anglais
ISBN 10 
3659832324
ISBN 13 
9783659832321
Reliure
Broché
Nombre de pages
72
Coordonnées du fabricant
non disponible
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