Articles liés à Expression of two insecticidal genes in Cotton: Development...

Expression of two insecticidal genes in Cotton: Development of Transgenic Cotton with sustained resistance against targeted insect pests - Couverture souple

Bakhsh, Allah; Husnain, Tayyab

 
9783844389203: Expression of two insecticidal genes in Cotton: Development of Transgenic Cotton with sustained resistance against targeted insect pests
Couverture souple
ISBN 10 : 3844389202 ISBN 13 : 9783844389203
Editeur : LAP LAMBERT Academic Publishing, 2011

Synopsis

Spatio-temporal expression of insecticidal gene (Cry1Ac& Cry2A) in pre existing lines of transgenic cotton was studied. Seasonal decline in expression of genes differed significantly among different cotton lines tested in the field conditions. The leaves of the Bt cotton plants were found to have the highest levels of toxin expression followed by squares, bolls, anthers and petals. Expression of the genes decreased consistently with the age of plants. Toxin expression in fruiting parts was not enough to confer full resistance against bollworms. The reduction in efficacy of transgenic cotton plants late in the season was attributed to reduction in promoter activity. For this purpose, Rubisco small subunit promoter was isolated from Gossypium arboreum that was further cloned upstream of Cry1Ac in expression vector pCAMBIA 1301. A local cotton cultivar was transformed with Cry1Ac driven by rbcS promoter. The same cotton cultivar was also transformed with Cry1Ac gene driven by 35SCaMV promoter to compare the expression pattern of insecticidal gene under two different promoters. The results showed that rbcS is an efficient promoter to drive Bt gene expression in cotton.

Les informations fournies dans la section « Synopsis » peuvent faire référence à une autre édition de ce titre.

Présentation de l'éditeur

Spatio-temporal expression of insecticidal gene (Cry1Ac& Cry2A) in pre existing lines of transgenic cotton was studied. Seasonal decline in expression of genes differed significantly among different cotton lines tested in the field conditions. The leaves of the Bt cotton plants were found to have the highest levels of toxin expression followed by squares, bolls, anthers and petals. Expression of the genes decreased consistently with the age of plants. Toxin expression in fruiting parts was not enough to confer full resistance against bollworms. The reduction in efficacy of transgenic cotton plants late in the season was attributed to reduction in promoter activity. For this purpose, Rubisco small subunit promoter was isolated from Gossypium arboreum that was further cloned upstream of Cry1Ac in expression vector pCAMBIA 1301. A local cotton cultivar was transformed with Cry1Ac driven by rbcS promoter. The same cotton cultivar was also transformed with Cry1Ac gene driven by 35SCaMV promoter to compare the expression pattern of insecticidal gene under two different promoters. The results showed that rbcS is an efficient promoter to drive Bt gene expression in cotton.

Biographie de l'auteur

Dr. Allah Bakhsh was born in Khanewal,(Pakistan)on April 15,1981. He completed four years honor degree in Agricultural sciences majoring in Plant Breeding and Genetics from University of Agriculture, Faisalabad, Pakistan in 2004. Afterwards, he joined CEMB, University of the Punjab, Lahore, Pakistan and completed his PhD in Molecular Biology.

Les informations fournies dans la section « A propos du livre » peuvent faire référence à une autre édition de ce titre.

Éditeur
LAP LAMBERT Academic Publishing
Date d'édition
2011
Langue
anglais
ISBN 10 
3844389202
ISBN 13 
9783844389203
Reliure
Broché
Nombre de pages
176
Coordonnées du fabricant
non disponible
Personne responsable
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