humana press apr 2011 (26 résultats)

Buch. Etat : Neu. Neuware -With its complex and extensively regulated metabolism, the study of the RNA lifecycle demands tools that allow for the localization of RNAs to be observed either in an in situ setting or, preferably, under in vivo conditions. In RNA Detection and Visualization: Methods and Protocols, the best and brightest investigators provide an up-to-date and in-depth description of basic methods and protocols used for detecting and visualizing mRNAs in both fixed and live cells, from bacteria to mammals. For novices and experts alike, this mix of classic in situ hybridization and advanced live imaging techniques, cell fractionation and affinity purification procedures, and bioinformatics tools gives researchers the most complete and extensive array of research aids possible. As a volume written in the highly successful Methods in Molecular Biology¿ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and expert tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, RNA Detection and Visualization: Methods and Protocols offers well-honed techniques in order to inspire researchers around the world to further our knowledge of the vital biological significance of RNA.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 512 pp. Englisch.

Buch. Etat : Neu. Neuware -Blood science has become a cornerstone of multiple disciplines, including clinical chemistry, disease diagnosis, and therapeutic monitoring. Over the past decade, we have witnessed the advent of increasingly powerful proteomics technologies that allow greater fundamental insights into the blood proteome. These technological improvements have, in part, fuelled the quest for the discovery of novel blood-based biomarkers of disease. Serum/Plasma Proteomics: Methods and Protocols is a comprehensive resource of protocols for areas, pre-analytical through to analytical, of plasma and serum proteomics. Divided into five convenient sections, this detailed volume covers fractionation strategies for in-depth blood proteome analysis, defined procedures for blood collection, handling and storage, detailed protocols for performing both antibody-based and non-antibody based quantitative assays, proteome analysis of blood cell compartments, circulating nanomebraneous vesicles and blood-related fluids, and finally data management, statistical design, and bioinformatic challenges. This book, contributed to by leading experts in the field, provides a valuable foundation for the development and application of blood-based proteomics. Written in the highly successful Methods in Molecular Biology¿ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.Authoritative and easily accessible, Serum/Plasma Proteomics: Methods and Protocols, with its well-honed methodologies, seeks to serve both professionals and investigators new to thefield in an effort to further our knowledge of this fundamental science.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 412 pp. Englisch.

Buch. Etat : Neu. Neuware -Due to their unique size-dependent properties, nanomaterials have the potential to revolutionize the detection, diagnosis, and treatment of disease by offering superior capabilities compared to conventionally-used materials. Biomedical Nanotechnology: Methods and Protocols brings together experts from a wide variety of fields to provide a practical overview of biomedical nanotechnology, from the conception of novel materials in the laboratory to the application of such structures in the clinic. After a brief introductory chapter, the first section consists of protocol chapters which provide hands-on information on the synthesis of a variety of solution-phase and surface-bound nanomaterials and their application in sensing, imaging, and/or therapeutics, while the second section consists of a series of case studies and review chapters that discuss the toxicology of nanomaterials, the regulatory pathways to US Food and Drug Administration (FDA) approval of these materials, their patenting, marketing, and commercialization, and the legal and ethical issues surrounding their use. Written in the highly successful Methods in Molecular Biology¿ series format, many chapters include introductions to their respective topics, lists of the necessary materials, step-by-step, readily reproducible protocols, and insightful tips on troubleshooting and avoiding known pitfalls.Cutting-edge and authoritative, Biomedical Nanotechnology: Methods and Protocols surveys this exciting field from the most vital angles in order to provide a comprehensive reference for scientists and researchers of all different backgrounds looking to utilize the numerous versatile applications of nanomaterial technologies.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 448 pp. Englisch.

Buch. Etat : Neu. Neuware -Whether the question is one of basic cell survival, or whether it is being used to correlate cell number to some other factor such as matrix synthesis, an estimate of cell viability is universally required. In Mammalian Cell Viability: Methods and Protocols, experts in the field describe methods from the most basic which can be performed in any laboratory, to some which require specific pieces of equipment. Initially focusing on methods for monolayer and suspension cells, later chapters describe methods for determining viability within tissue sections and 3 dimensional culture systems. Finally, methods requiring highly specialized equipment are described in order to explain what is possible. Written in the highly successful Methods in Molecular Biology¿ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and vital tips on troubleshooting and avoiding known pitfalls.Practical and adaptable, Mammalian Cell Viability: Methods and Protocols serves as a self-contained laboratory manual useful to both experienced researchers and those new to this incredibly important and influential field.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 252 pp. Englisch.

Buch. Etat : Neu. Neuware -Regulation of gene expression, a major determinant of gene activity, occupies a central place in molecular biology. Yeast Genetic Networks: Methods and Protocols covers approaches to the systems biological analysis of small-scale gene networks in yeast. Divided into four convenient sections, this detailed volume discusses the methods used to analyze gene expression quantitatively, presents a collection of mathematical and computational tools to analyze stochasticity, adaptation, sensitivity in signal transmission, and oscillations in gene expression, provides instructional methods on how to utilize the tools of quantitative genetics to identify genes that regulate stochasticity and oscillations in gene expression, and concludes with a section devoted to the analysis of conserved gene expression systems and networks in different fungal species. Written in the highly successful Methods in Molecular Biology¿ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.Authoritative and accessible, Yeast Genetic Networks: Methods and Protocols serves as an ideal and valuable resource for both novices and experienced researchers.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 356 pp. Englisch.

Buch. Etat : Neu. Neuware -The rapid progression of genetics and molecular biology has turned chromosomal engineering from science fiction to reality, with the successful production of transgenic animals with engineered chromosomes and chromosomes developed for pharmaceutical protein production which are now ready for the medical industry. Mammalian Chromosome Engineering: Methods and Protocols provides the reader with up-to date information on this rapidly evolving field and strives to take the reader into the exciting realm of chromosomal engineering from the basic principles to the practical applications of these new technologies. The five overview and ten protocol chapters cover the engineering of chromosomes with extrachromosomal vectors and transposon systems, the manipulation of naturally occurred minichromosomes, the generation and engineering of synthetic artificial chromosomes, and the induced de novo platform artificial chromosome system. Written in the highly successful Methods in Molecular Biology¿ series format, protocols chapters contain brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Mammalian Chromosome Engineering: Methods and Protocols serves as a bench-side resource for current protocols and aims to help scientists to explore the many prospects for future research and vital applications.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 268 pp. Englisch.

Buch. Etat : Neu. Neuware -There is a widespread consensus that use of antioxidants as a therapeutic approach may counteract free radical mediated pathologies. However, the role of antioxidants in normal physiology and redox signaling is still in its infancy. Since oxidative stress is related to various diseases and pathologies, scientists are eager to study the disease in humans, but it is not always ethical to study all the aspects of the disease in humans. Thus, it becomes mandatory to study the disease process and the mechanisms behind it through experimental models which generally involve animals, in vitro/cell culture studies, primates and even humans to a certain extent. Studies on Experimental Models contains data on the experimental models or review of such models of oxidative stress in various diseases. It is structured into six sections, which are as follows: diabetes, cardiovascular, neurology, ocular diseases, toxicology/environmental and in vitro/tissue culture. Each section presents a sketch of models in humans, animals and in vitro methods. Taken together, they comprise a valuable reference for basic and clinical scientists, one aimed at contributing to the advancement of oxidative stress research using appropriate animal models.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 720 pp. Englisch.

Buch. Etat : Neu. Neuware -Molecular Diagnostics in Dermatology and Dermatopathology presents the basics of molecular biology and molecular diagnostic methods most commonly used in the clinical laboratory, with an emphasis on the concepts and testing most relevant to dermatological diseases. Topics include the integration of newer diagnostic and prognostic techniques with ¿traditional¿ histologic approaches, and discussions of regulatory, ethical, legal, economic issues and ¿newer¿ technologies. This important diagnostic tool outlines the clinically relevant uses (i.e.; diagnostic, staging and/or prognostic) applications of these techniques in the field of dermatology. Molecular studies that investigate the pathogenesis of skin diseases will be excluded, unless they also have a direct diagnostic utility. The book will be of interest to practicing pathologists, dermatology and pathology residents, dermatologists, and dermatopathologists.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 496 pp. Englisch.

Buch. Etat : Neu. Neuware -The rationale for using intraoperative irradiation (IORT) is based on the realization that tolerable doses of eternal beam radiation are often insufficient to achieve control of locally advanced malignancies. In these instances, the IORT component of treatment becomes the optimal conformal technique of irradiation, since dose-limiting organs or structures can either be surgically displaced or protected by placement of lead shielding.This fully revised and expanded second edition is of interest to those with intraoperative electron (IOERT) capabilities, high-dose-rate brachytherapy (HDR-IORT) capabilities, or both. Techniques, indications, and results are discussed by disease site. Each chapter is dual authored by a radiation oncologist and a surgeon, giving a balanced presentation of clinical scenarios. Issues of basic science and physics are also covered, and a notable chapter on normal tissue tolerance is included.Intraoperative Irradiation: Techniques and Results, Second Edition is a superb compilation, providing essential cutting-edge knowledge. It is a foundation for physicians as IORT develops and becomes more widely available.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 548 pp. Englisch.

Buch. Etat : Neu. Neuware -Medical research made huge strides in treating heart disease in the 20thcentury, from drug-eluding stents to automatic internal defibrillators. Public awareness of the dangers of heart disease has never been more pervasive. Now, though, ten years into a new millennium, scientists are gearing up for the next great challenges in tackling this pervasive condition. Cell therapy is going to be a key weapon in the fight against heart disease. It has the potential to address many cardiovascular conditions. From heart failure to atrioventricular nodal dysfunction, the young but promising field of cell therapy is set to play a significant role in developing the cures that the upcoming decades of hard work will yield.Regeneratingthe Heart: Stem Cells and the Cardiovascular System organizes the fieldinto a digestible body of knowledge. Its four sections cover mechanicalregeneration, electrical regeneration, cardiac tissues and in vivo stem celltherapies. An array of talented researchers share the fruits of their laborswith chapters covering such crucial issues as the cardiogenic potential ofvarying stem cell types, the ways in which they might be used to tacklearrhythmias, their possible application to biological replacements for cardiactissues such as valves, and the varying approaches used in the in vivoevaluation of stem cell therapies, including methods of delivering stem cellsto the myocardium.Thiscomprehensive survey of an area of research with such exciting potential is aninvaluable resource both for veteran stem cell researchers who need to monitorfresh developments, and for newly minted investigators seeking inspirationalexamples.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 572 pp. Englisch.

Buch. Etat : Neu. Neuware -Mesenchymal Stem Cells have seen an unprecedented level of interest in the last decade, primarily due to their relative ease of isolation, the large numbers of cells present in the adult, and the ability to propagate these cells in culture. In Mesenchymal Stem Cell Assays and Applications, expert researchers from across the globe explore the latest techniques to propagate, characterize, and engineer this special cell type. Chapters outline a set of protocols and assays used by leading investigators in the field, providing standards that can be applied by all researchers to the population of cells used in their experiments. Composed in the highly successful Methods in Molecular Biology¿ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.Ground-breaking and current, Mesenchymal Stem Cell Assays and Applications is a necessary handbook for all researchers working with this ambiguous population of cells.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 552 pp. Englisch.

Buch. Etat : Neu. Neuware -With many recent advances, cancer cell culture research is more important than ever before. This timely edition of Cancer Cell Culture: Methods and Protocols covers the basic concepts of cancer cell biology and culture while expanding upon the recent shift in cell culture methods from the generation of new cell lines to the use of primary cells. There are methods to characterize and authenticate cell lines, to isolate and develop specific types of cancer cells, and to develop new cell line models. Functional assays are provided for the evaluation of clonogenicity, cell proliferation, apoptosis, adhesion, migration, invasion, senescence, angiogenesis, and cell cycle parameters. Other methods permit the modification of cells for transfection, drug resistance, immortalization, and transfer in vivo, the co-culture of different cell types, and the detection and treatment of contamination. In this new edition, specific emphasis is placed on safe working practice for both cells and laboratory researchers. These chapters contain the information critical to success ¿ only by good practice and quality control will the results of cancer cell culture improve. Written in the successful Methods in Molecular Biology¿ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.Authoritative and accessible, Cancer Cell Culture: Methods and Protocols serves as a practical guide for scientists of all backgrounds and aims to convey the appropriate sense of fascination associated with this research field.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 516 pp. Englisch.

Taschenbuch. Etat : Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -Table 1 Cancer is the second most common cause of death in Americans (see cdc.gov). Colorectal cancer kills more Incidence and Mortality of the Five Most Common Gastrointestinal Malignancies Americans than any other malignancy except for lung cancer. The incidences and mortalities of the major gastrointestinal a a Site Incidence Mortality (GI) malignancies are shown in Table 1. Taken as a group, the five most common GI malignancies account for more cancers Colorectum 53.9 21.6 and more cancer deaths than for any other site. Pancreas 11.1 10.6 Stomach 9.1 4.9 Flexible endoscopy has given physicians unprecedented Liver/intrahepatic bile ducts 6.2 4.4 access to the GI tract. The ability to endoscopically visu- Esophagus 4.5 4.3 alize, biopsy, and apply therapy has had implications for the management of all the major GI malignancies. Accepted Data from SEER database 1992-2002 (seer.cancer.gov). applications of endoscopy range from detection of mal- a Per 100,000. 400 pp. Englisch.