springer new york springer new york mai 2016 (38 résultats)

Buch. Etat : Neu. Neuware -This volume providesa sound basis for the molecular investigation of NLR function in health anddisease. Chapters focus on of innate immune receptors, ¿atypical¿inflammasomes, biochemical and novel bioluminescencetechniques for the measurement of IL-1bbioluminescent probe, biochemical and microscopytechniques, techniques to measure caspase-1 activation, cellfree systems for the study of inflammasome function, and inflammasomeactivation. Written in the highly successful Methodsin Molecular Biology series format, chapters include introductions to theirrespective topics, lists of the necessary materials and reagents, step-by-stepreadily reproducible laboratory protocols, and tips on troubleshooting andavoiding known pitfalls.Authoritativeand cutting-edge, NLR Proteins: Methodsand Protocols aims to ensure successful results in the further study ofthis vital field.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 272 pp. Englisch.

Buch. Etat : Neu. Neuware -This contributed volume presents the experiences, challenges, trends, and advances in Service Science from Japan¿s perspective. As the global economy becomes more connected and competitive, many economies depend the service sector on for growth and prosperity. A multi-disciplinary approach to Service Science can potentially transform service industries through research, education, and practice. Offering a forum for best practices in Service Science within Japan, the volume benefits its audience by sharing viewpoints from a wide range of geographical regions and economies. The book is organized as follows:¿ Foundations of Service Science and the service industry sector¿ Public/Private sector partnerships, policies, trade in services, future prospects¿ Contributions from science, social science, management, engineering, design as well as industry sector perspectives¿ Road-maps, methodology, business development, strategiesand innovative models, application of information technology, performance measures, and service system design¿ Education and workforce development¿ Case studies from practice, research and educational community¿ Future Directions in JapanSpringer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 364 pp. Englisch.

Buch. Etat : Neu. Neuware -Inaddition to updating important core techniques this third edition presents newchapters on assessing leukocyte involvement in angiogenesis, lymphatic cell andpericyte isolation techniques, spheroid and arterial ring based in vitroassays, and on pericyte involvement in angiogenesis. Angiogenesis ProtocolsThird Edition also guides readers through new protocols on chorioallantoicmembrane models, corneal pocket assays to assess angio- and lymphangiogenesismodels of muscle angiogenesis, and use of zebrafish embryos to study vascularangiogenesis and senescence. Written in the highly successful Methods inMolecular Biology series format, chapters include introductions to theirrespective topics, lists of the necessary materials and reagents, step-by-stepreadily reproducible laboratory protocols, and tips on troubleshooting andavoiding known pitfalls.Authoritativeand practical, Angiogenesis Protocols, Third Edition provides not only apractical handbook for key techniques, but also an informative and enjoyableread for all those interested, no matter how directly, in angiogenesis.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 416 pp. Englisch.

Buch. Etat : Neu. Neuware -The third edition of thisvolume is aimed at providing both beginners and more experienced researchers achoice of methods to isolate and analyze dendritic cells(DC). An introductoryreview provides an overview of recent advances in the characterization of DCsubsets in mouse and human. While additional chapters provide methods toculture human and mouse dendritic cells, protocols for the isolation ofdendritic cells, the isolation of dendritic cell progenitors from mouse, andthe purification of dendritic cells from human blood. Written in the highlysuccessful Methods in Molecular Biology series format, chapters includeintroductions to their respective topics, lists of the necessary materials andreagents, step-by-step, readily reproducible laboratory protocols, and tips ontroubleshooting and avoiding known pitfalls.Authoritative andcutting-edge, Dendritic CellProtocols, Third Edition aims to ensure successful results in the furtherstudy of this vital field.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 336 pp. Englisch.

Buch. Etat : Neu. Neuware -This book discusses the molecular, biological, pathological, and clinical aspects of melanoma, with special emphasis in the new concepts of melanoma genetics. A multidisciplinary group of experts in Genetics, Dermatology, Pathology, and Melanoma Medical Oncology contribute state-of-the-art knowledge in melanoma research and clinical management, not only exposing the current status of knowledge of the topics but also providing their personal experiences and ideas about the future and potential practical application of the genetic aspects of melanoma. During the last few years we have witnessed an impressive amount of discoveries in the field of melanoma genetics which have changed our approach in understanding the pathogenesis and treatment of this lethal disease. Genetics of Melanoma is a practical approach to melanoma genetic mechanisms and their application in the diagnosis and treatment of this malignancy. It is an essential source of updated information and a powerful tool for clinicians, pathologists, and basic scientists who wish to understand, apply, and investigate the multiple new aspects of melanoma genetics.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 304 pp. Englisch.

Buch. Etat : Neu. Neuware -Thisvolume covers multidisciplinary approaches on various aspects of ChikungunyaVirus (CHIKV) research that was brought together from leading laboratoriesacross the globe. Topics covered include techniques in clinical and diagnosticvirology, basic protocols in cell and virus culture, bioinformatics, andproteomics approaches in cellular response studies. Chikungunya Virus:Methods and Protocols also discusses methods in immunology and animal modelstudies, as well as different strategies of antivirals and vaccine developmentfor therapeutics against CHIKV infection. Written in the highly successful Methodsin Molecular Biology series format, chapters include introductions to theirrespective topics, lists of the necessary material and reagents, step-by-stepreadily reproducible laboratory protocols, and tips on troubleshooting and avoidingknown pitfalls.Thorough and practical, Chikungunya Virus: Methods and Protocols is auseful and comprehensive resource for CHIKV researchers and anyone interestedin learning more about the Chikungunya Virus.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 352 pp. Englisch.

Buch. Etat : Neu. Neuware -Presentingstate-of-the-art protocols to study gap junctions, this detailed book firstfocuses on the use of methods and tools to investigate the different aspects ofconnexin expression and gap junction regulation. The second part of the volumedescribes several methods to probe gap junction functionality as such. Writtenin the highly successful Methods in Molecular Biology series format, chaptersinclude introductions to their respective topics, lists of the necessarymaterials and reagents, step-by-step and readily reproducible laboratoryprotocols, and key tips on troubleshooting and avoiding known pitfalls.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 252 pp. Englisch.

Buch. Etat : Neu. Neuware -This volume is a collection ofresearch methods, techniques, and approaches to investigate the molecularbiology of West Nile Virus (WNV). Chapters in the book cover many facets ofWNV, such as propagation and titration of WNV in vero cells, examination of WNVneuroinvasion and neuropathogenesis in the central nervous system of a murinemodel, field surveillance methods for WNV, and in vitro and in vivoblood-brain barrier models to study WNV pathogenesis. A brief introductionalong with a discussion on WNV laboratory safety are also included. Written inthe highly successful Methods in Molecular Biology series formatchapters includes introduction to their respective topics, lists of thenecessary materials and reagents, step-by-step, readily reproducible laboratoryprotocols, and tips on troubleshooting and avoiding known pitfalls.Practical and thorough, West Nile Virus will interest virologistsmolecular biologists, microbiologists, and other scientists working with WNV.This book is a comprehensive guide for those conducting research in the fieldof WNV biology, including life cycles, pathogenesis, impact of infection, andpossible treatment/prevention development.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 308 pp. Englisch.

Buch. Etat : Neu. Neuware -With genetic engineering, systems explored in this book now exist allowingfor the simple, efficient, and near universally precise genetic manipulationdirectly in any organism, including the mouse. Herein, these models are appliedto a wide field of disease areas, including diabetes, cardiovascular diseaseskin disorders, cancer, neurodegenerative and neuromuscular diseases, retinaldisorders, as well as various behavioral models. Written for the highlysuccessful Methods in Molecular Biologyseries, chapters include introductions to their respective topics, lists of thenecessary materials and reagents, step-by-step, readily reproducible laboratoryprotocols, and tips on troubleshooting and avoiding known pitfalls.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 448 pp. Englisch.

Buch. Etat : Neu. Neuware -This book grew out of the need to provide students with a solid introduction to modern fluid dynamics. It offers a broad grounding in the underlying principles and techniques used, with some emphasis on applications in astrophysics and planetary science.The book comprehensively covers recent developments, methods and techniques, including, for example, new ideas on transitions to turbulence (via transiently growing stable linear modes), new approaches to turbulence (which remains the enigma of fluid dynamics), and the use of asymptotic approximation methods, which can give analytical or semi-analytical results and complement fully numerical treatments. The authors also briefly discuss some important considerations to be taken into account when developing a numerical code for computer simulation of fluid flows.Although the text is populated throughout with examples and problems from the field of astrophysics and planetary science, the text is eminently suitable as a general introduction to fluid dynamics. It is assumed that the readers are mathematically equipped with a reasonable knowledge in analysis, including basics of ordinary and partial differential equations and a good command of vector calculus and linear algebra.Each chapter concludes with bibliographical notes in which the authors briefly discuss the chapter's essential literature and give recommendations for further, deeper reading. Included in each chapter are a number of problems, some of them relevant to astrophysics and planetary science. The book is written for advanced undergraduate and graduate students, but will also prove a valuable source of reference for established researchers.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 704 pp. Englisch.

Taschenbuch. Etat : Neu. Neuware -The cognitive and behavioral implications of sleep deprivation have been noted in the medical literature for many years. In addition, emerging research continues to demonstrate the contribution of sleep deprivation to some of the most common and costly health conditions today.Sleep Deprivation and Disease provides clinically relevant scientific information to help clinicians, public health professionals, and researchers recognize the ramifications of sleep deprivation across a broad spectrum of health topics. This timely reference covers sleep physiology, experimental approaches to sleep deprivation and measurement of its consequences, as well as health and operational consequences of sleep deprivation. Clinical challenges and areas of uncertainty are also presented in order to encourage future advancements in sleep medicine and help patients avoid the outcomes associated with the myriad causes of sleep deprivation.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 304 pp. Englisch.

Buch. Etat : Neu. Neuware -This title is a comprehensive text that addresses key aspects of nanomedicine such as properties occurring at the nanoscale that have unique medical effects, great molecular knowledge of the human body and disease processes, and apparent clinical translation as opposed to narrow insufficient texts that address only a few topics and attempt to ¿rebrand¿ established drug delivery. It will clearly define the field which is needed due to the immaturity and broad nature of the field. The book is aligned with both the USA and European roadmaps for nanomedicine and will address initiatives taken in Asia that ensures timely and relevant content. In-depth chapters ensure each section is adequately covered. The nanopharmaceutical section focuses on novel drug delivery systems relevant to nanomedicine and the book has an extensive section on immune recognition at the nanoscale which has implications for in vivo applications of nanomedicines. ¿Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 396 pp. Englisch.

Buch. Etat : Neu. Neuware -This volume includes a series ofprotocols focused on mitotic spindle assembly and function. The methods coveredin this book feature a broad range of techniques from basic microscopy to thestudy of spindle physiologies relevant to cancer. These methods can be appliedto diverse model systems that range from the cell-free Xenopus eggextract system to the moss Physcomitrella patens, in an effort todemonstrate the key contributions made by researchers using multiple modelorganisms. Chapters in The Mitotic Spindle: Methods and Protocols integrate cutting-edge technologies that have only become available due to thecross-disciplinary efforts, such as ATP analogue sensitive inhibition ofmitotic kinases. Written in the highly successful Methods in Molecular Biologyseries format, chapters include introductions to their respective topicslists of the necessary materials and reagents, step-by-step, readilyreproducible laboratory protocols, and tips ontroubleshooting and avoidingknown pitfalls.Thorough and informative, TheMitotic Spindle: Methods and Protocols, is a valuable resource forresearchers who are new to mitosis or are already experts in the field.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 440 pp. Englisch.

Buch. Etat : Neu. Neuware -This volume provides a wide rangeof protocols used in studying the nuclear envelope, with special attention tothe experimental adjustments that may be required to successfully investigatethis complex organelle in cells from various organisms. The Nuclear Envelope:Methods and Protocols is divided into five sections: Part I ¿ Nuclear EnvelopeIsolation; Part II ¿ Nuclear Envelope Protein Interactions, Localization, andDynamics; Part III ¿ Nuclear Envelope Interactions with the Cytoskeleton; PartIV ¿ Nuclear Envelope-Chromatin Interactions; and Part V ¿ Nucleo-CytoplasmicTransport. Many of the modifications discussed in this book have only beencirculated within laboratories that have conducted research in this field formany years. Written in the highly successful Methods in Molecular Biologyseries format, chapters include introductions to their respective topics, listsof the necessary materials and reagents, step-by-step, readily reproduciblelaboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting edge and thorough, The Nuclear Envelope: Methods and Protocols is a timely resource for researchers who have joined this dynamic and rapidly growing field.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 540 pp. Englisch.

Buch. Etat : Neu. Neuware -This book explores the regenerative properties of fetal stem cells, from feto-maternal cell traffic through perinatal stem cells, with a discussion of key topics including stem cell banking, drug screening, in utero stem cell transplantation and ethical considerations. The expertly authored chapters also delve into embryonic, amniotic membrane, and umbilical cord blood stem cells; fetal development models; fetal cell reprogramming; culture methods; disease models; perinatal gene therapy, and more. These chapters are grouped into four sections, each discussing a separate prenatal stem cell population and providing fascinating historical contexts for our knowledge of these systems.Featuring a foreword written by the renowned Dr. Joseph Vacanti of the Harvard Stem Cell Institute, Fetal Stem Cells in Regenerative Medicine: Principles and Translational Strategies is a welcome and timely contribution to the Stem Cell Biology and Regenerative Medicine series. It is essential reading for scientists and researchers, clinicians and residents, and advanced students involved in stem cells, regenerative medicine, tissue engineering, and related disciplines such as embryology.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 476 pp. Englisch.

Buch. Etat : Neu. Neuware -Many breakthroughs in biological research and translational healthcare advancements have been achieved by studying the response of biological systems to extreme environments. The spaceflight platform provides a unique environment where researchers can explore fundamental questions into cellular and molecular response mechanisms to unveil novel insight into human health and disease. Since the physical force of gravity has shaped the architecture of all biological systems on our planet, spaceflight provides the opportunity to see life in a new adaptational mode - in response to reduced gravity. This enables investigations into the effects of the microgravity environment and associated changes in mechanical forces on mammalian cells/tissues and microbial pathogens, to bring novel insight into disease mechanisms, which are not discernable using conventional experimental approaches.Research using spaceflight platforms represents a paradigm shift in how we observe life processes and is on the leading edge of research discoveries into cellular and molecular mechanisms of health and disease. By incorporating the views of leading authors,this book highlights landmark discoveries and advances in mammalian cellular and microbiology research in both true spaceflight and ground-based spaceflight analogue environments for scientists and students alike who are interested in the influence of physical forces on mammalian and microbial cells, how this impactstransitionbetween normal homeostasis and disease, and basic mechanisms of adaptation to low gravity environments.To provide a thorough understanding of this research, this book covers a range of topics including:(i)description the physical forces interacting with cells in microgravity and microgravity analogue environments(ii)how alterations in these cellular forces impact human physiology, specifically immune function(iii)useof these environments to develop organotypic three-dimensional (3-D) tissue culture models as predictive human surrogates for organogenesis and disease research, and(iv)microbial pathogen responses to culture in these environments, focusing on infectious diseaseCollectively, this information reflects a critical step in preparation for long-duration human space exploration, advances our knowledge of basic biological processes and mechanisms important to understand normal function and disease, and may lead to new strategies for treatment and prevention.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 320 pp. Englisch.

Buch. Etat : Neu. Neuware -This volume contains collection of Natural Killer Cell methodologies relevant for both basic and translational research. These methodologies present new developments in the natural killer (NK) cell field, such as understanding the influence of NK cells metabolism on its function, identifying complexity of NK cell subsets through mass cytometry, and determining the emergence of memory NK cells in murine model of MCMV infection. Methods that study NK cell migration and cytotoxicity through endpoint analysis or live single cell imaging are also discussed. Chapters also describe methods pertaining to translational application of NK cells, such as ex vivo expansion of NK cells on K562 cell lines genetically modified to express either membrane bound IL-15 or membrane bound IL-21, large scale NK cell culture, current techniques for engineering NK cells to express chimeric antigen receptors or chemokine receptors using retroviral vectors,electroporation of mRNA, and the natural phenomenon of trogocytosis. Written in the highly successful Methods in Molecular Biology series format, these chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting edge and thorough, Natural Killer Cells: Methods and Protocols is a valuable resource for researchers who not only want to understand mechanisms that govern NK cell behavior and diversity, but also for those who want to understand how to systematically evaluate NK cells for adoptive immunotherapy applications.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 384 pp. Englisch.

Buch. Etat : Neu. Neuware -This volumepresents detailed laboratory protocols for invitro synthesis of mRNA with favorable properties, its introduction intocells by a variety of techniques, and the measurement of physiological andclinical consequences such as protein replacement and cancer immunotherapy. Synthetictechniques are described for structural features in mRNA that provide investigationaltools such as fluorescence emission, click chemistry, photo-chemicalcrosslinking, and that produce mRNA with increased stability in the cell, increased translational efficiency, and reducedactivation of the innate immune response. Protocols are described for clinicalapplications such as large-scale transfection of dendritic cells, production ofGMP-grade mRNA, redirecting T cell specificity, and use of molecularadjuvants for RNA vaccines. Writtenin the highly successful Methods inMolecular Biology series format, chapters include introductions to theirrespective topics, lists of the necessarymaterials and reagents, step-by-steplaboratory protocols, and tips on troubleshooting and avoiding known pitfalls.SyntheticmRNA: Production, Introduction into Cells, and Physiological Consequences is a valuable and cutting-edge resourcefor both laboratory investigators and clinicians interested in this powerfuland rapidly evolving technology.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 332 pp. Englisch.

Buch. Etat : Neu. Neuware -This volume aims to outline the current status of the Mesenchymal Stem Cells(MSC) field in regenerative medicine and to propose clear and reproducible protocols to better define the identity, function and use of these cells that are today, more than ever, ¿under the spotlight¿. Mesenchymal Stem Cells: Methods and Protocols, Second Edition is organized into four sections. The first guides the reader through a series of state-of-the-art reviews summarizing the use of MSC for the treatment of various diseases. The other three sections are a collection of methodological chapters covering several aspects: isolation and characterization of MSC; expansion of MSC for clinical use; production and characterization of the MSC secretome. Written in the highly successful Methods in Molecular Biology series format, the method chapters include introductions to their respective topics, complete lists of the necessary materials and reagents, step-by-step, readily reproduciblelaboratory protocols, and tips on troubleshooting which will help the researcher to avoid known pitfalls.Authoritative and cutting-edge, Mesenchymal Stem Cells: Methods and Protocols, Second Edition, aims to ensure successful results in the further study of this vital field.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 592 pp. Englisch.