springer us springer new york jul 2018 (52 résultats)

Taschenbuch. Etat : Neu. Neuware -Psychological, social, and spiritual care is as important as physical care at the end of life. Yet caregivers often feel ill-equipped to give that nonphysical care. This book shows how to do it. The book addresses all caregivers who attend dying patients: doctors, nurses, chaplains, clergy in the pastorate, social workers, clinical psychologists, family caregivers, and others. It covers such topics as the functional and emotional trajectories of dying; the varied approaches of patients and caregivers to end-of-life decisions; culturally based beliefs about dying; the differences between depression and grief; and people¿s views about the right time to die, the death experience itself, and the afterlife. For each topic the book introduces core concepts and summarizes recent research about them. The book presents much of its material in readable tables for easy reference; applies the material to real-life cases; lists the main ¿take home¿ points for each chapter; and gives references for additional reading. The book helps caregivers anticipate the reactions of patients and survivors to end-of-life traumas and suggests how caregivers can respond insightfully and compassionately. At the same time the book challenges caregivers to think through their own views about death and dying. This book, therefore, is a must-read for all caregivers¿professional and nonprofessional alike¿who strive to give their patients comprehensive, high-quality end-of-life care.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 504 pp. Englisch.

Taschenbuch. Etat : Neu. Neuware -This volume provides readers with detailed protocols covering the main cancer cytogenetics techniques needed for clinical utilization and research purposes. The chapters in this book cover topics such as chromosome preparation for myeloid malignancies; chromosome bandings; fluorescence in situ hybridization probe preparation; array-based comparative genomic hybridization; and cytogenetic nomenclature and reporting. The updated reviews on chromosomal abnormalities in hematological malignancies are excellent guides for cytogenetics data interpretations and specific malignant diseases correlation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and comprehensive, Cancer Cytogenetics: Methods and Protocolsis a valuable resource for the novice in cytogenetics because it provides helpful guiding protocols, but it¿s also great for those who are already engaged in the field and are looking for some technical hints.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 352 pp. Englisch.

Taschenbuch. Etat : Neu. Neuware -Given the rapidly developing area of evolutionary medicine and public health, The Arc of Life examines ways in which research conducted by biological anthropologists can enrich our understanding of variation in human health outcomes. The book aimsnot only to showcase the perspective that biological anthropologists bring to the burgeoning field of evolutionary medicine, but to underscore the context of human life history -- especially the concept of evolutionary trade-offs and the ensuing biological processes that can affect health status over the life course. This dual emphasis on life history theory and life cycle biology will make for a valuable and unique, yet complementary, addition to books already available on the subject of evolution and health.The book consolidates diverse lines of research within the field of biological anthropology, stimulates new directions for future research, and facilitates communication between subdisciplines of human biology operating at the forefrontof evolutionary medicine.¿Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 208 pp. Englisch.

Taschenbuch. Etat : Neu. Neuware -This second edition provides updated and expanded chapters covering a broad sampling of useful and current methods in the rapidly developing and expanding field of bioinformatics. Bioinformatics, Volume II: Structure, Function, and Applications, Second Edition is comprised of three sections: Structure, Function, Pathways and Networks; Applications; and Computational Methods. The first section examines methodologies for understanding biological molecules as systems of interacting elements. The Applications section covers numerous applications of bioinformatics, focusing on analysis of genome-wide association data, computational diagnostic, and drug discovery. The final section describes four broadly applicable computational methods that are important to this field. These are: modeling and inference, clustering, parameterized algorithmics, and visualization. As a volume in the highly successful Methods in Molecular Biology series, chapters feature the kind of detailand expert implementation advice to ensure positive results.Comprehensive and practical, Bioinformatics, Volume II: Structure, Function, and Applications is an essential resource for graduate students, early career researchers, and others who are in the process of integrating new bioinformatics methods into their research.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 440 pp. Englisch.

Taschenbuch. Etat : Neu. Neuware -This volume presents some of the research topics discussed at the 2014-2015 Annual Thematic Program Discrete Structures: Analysis and Applications at the Institute of Mathematics and its Applications during the Spring 2015 where geometric analysis, convex geometry and concentration phenomena were the focus.Leading experts have written surveys of research problems, making state of the art results more conveniently and widely available. The volume is organized into two parts. Part I contains those contributions that focus primarily on problems motivated by probability theory, while Part II contains those contributions that focus primarily on problems motivated by convex geometry and geometric analysis.This book will be of use to those who research convex geometry, geometric analysis and probability directly or apply such methods in other fields.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 636 pp. Englisch.

Taschenbuch. Etat : Neu. Neuware -Thisvolume provides a variety of protocols to analyze various epigeneticchanges, including differential expression of non-coding RNAs, changes in DNAmethylation, and histone modifications in plants. Chapters detail protocolswith different degrees of complexity, and describe bioinformatics approachesfor data processing and analysis. Written in the highly successful Methodsin Molecular Biology series format, chapters include introductions to theirrespective topics, lists of the necessary materials and reagents, step-by-stepreadily reproducible laboratory protocols, and tips on troubleshooting andavoiding known pitfalls.Authoritative andcutting-edge, Plant Epigenetics: Methods and Protocols, Second Editionaims to ensure successful results in the further study of this vital field.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 264 pp. Englisch.

Taschenbuch. Etat : Neu. Neuware Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 260 pp. Englisch.

Taschenbuch. Etat : Neu. Neuware Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 380 pp. Englisch.

Taschenbuch. Etat : Neu. Neuware -This book illustrates the current state-of-the-art in histamine research, with a focus on the appropriate methodologies to investigate the pharmacological properties and the therapeutic exploitation of HRs and their ligands. In addition, the range of techniques described provides an introduction to complementary cross-methodological disciplines beyond these fields. This multi-disciplinary approach is required to define the ¿decision gates¿ that determine the development of more effective and safer therapeutic options for many forms of highly prevalent and debilitating diseases, such as asthma, dementias, dermatitis, and arthritis. Written for the Methods in Pharmacology and Toxicology series, chapters concentrate on practical, hands-on protocols from experts in the techniques.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 536 pp. Englisch.

Taschenbuch. Etat : Neu. Neuware -This detailed volume presents a comprehensive technical overview of DNA nanotechnology with an emphasis on 3D DNA nanostructure design and applications. Coverage spans from basic design principles for DNA and RNA nanostructures to their cutting-edge applications in a variety of fields, with the book divided into basic DNA and RNA nanostructure design strategies as well as applications utilizing DNA nanostructures, including but not limited to nanomedicine, bioimaging, biosensing, nanoplasmonics, nanoelectronics, nanofabrication, crystallography, biophysics, and analytical chemistry. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 300 pp. Englisch.

Taschenbuch. Etat : Neu. Neuware -This detailed book provides methodological information on cardiac gene delivery, from classic to state-of-the-art technologies and techniques. Efficient, cardiac-specific, and safe vectors, as well as refined vector delivery methods, are key for successful cardiac gene transfer and eventually for improving patients¿ outcomes. Newer vectors and more efficient vector delivery methods have the potential to dramatically improve gene transduction efficacy, while novel gene manipulation techniques enforce the therapeutic power and broaden disease targets. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 368 pp. Englisch.

Taschenbuch. Etat : Neu. Neuware -This two-volume book is a valuable resource to students, researchers, scientists, commercial producers, consultants and policymakers interested in agriculture or plant sciences particularly in date palm biotechnology. Date Palm Biotechnology Protocols, Volume 1: Tissue Culture and Applications is comprised of 27 chapters covering adventitious organogenesis, somatic embryogenesis, contamination, hyperhydricity, acclimatization, cell suspension, protoplast and bioreactors, genetic transformation secondary metabolites, and abiotic stress. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Date Palm Biotechnology Protocols, Volume 1: Tissue Culture and Applications aims to supplement the previous volume and to provide precise stepwise protocols in the field of date palm biotechnology.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 368 pp. Englisch.

Taschenbuch. Etat : Neu. Neuware -This book provides comprehensive coverage of verification and debugging techniques for embedded software, which is frequently used in safety critical applications (e.g., automotive), where failures are unacceptable. Since the verification of complex systems needs to encompass the verification of both hardware and embedded software modules, this book focuses on verification and debugging approaches for embedded software with hardware dependencies. Coverage includes the entire flow of design, verification and debugging of embedded software and all key approaches to debugging, dynamic, static, and hybrid verification. This book discusses the current, industrial embedded software verification flow, as well as emerging trends with focus on formal and hybrid verification and debugging approaches.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 224 pp. Englisch.

Taschenbuch. Etat : Neu. Neuware -This detailed volume provides a toolbox for designing constructs, tackling expression and solubility issues, handling membrane proteins and protein complexes, and exploring innovative engineering of E. coli. The topics are largely grouped under four parts: high-throughput cloning, expression screening, and optimization of expression conditions, protein production and solubility enhancement, case studies to produce challenging proteins and specific protein families, as well as applications of E. coli expression. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 444 pp. Englisch.

Taschenbuch. Etat : Neu. Neuware -This book presents a comprehensive and self-contained treatment of the authors¿ newly developed scalable algorithms for the solutions of multibody contact problems of linear elasticity. The brand new feature of these algorithms is theoretically supported numerical scalability and parallel scalability demonstrated on problems discretized by billions of degrees of freedom. The theory supports solving multibody frictionless contact problems, contact problems with possibly orthotropic Trescäs friction, and transient contact problems. It covers BEM discretization, jumping coefficients, floating bodies, mortar non-penetration conditions, etc.The exposition is divided into four parts, the first of which reviews appropriate facets of linear algebra, optimization, and analysis. The most important algorithms and optimality results are presented in the third part of the volume. The presentation is complete, including continuous formulation, discretization, decomposition, optimality results, and numerical experiments. The final part includes extensions to contact shape optimization, plasticity, and HPC implementation. Graduate students and researchers in mechanical engineering, computational engineering, and applied mathematics, will find this book of great value and interest.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 360 pp. Englisch.

Taschenbuch. Etat : Neu. Neuware -This volume opens the world of free probability to a wide variety of readers. From its roots in the theory of operator algebras, free probability has intertwined with non-crossing partitions, random matrices, applications in wireless communications, representation theory of large groups, quantum groups, the invariant subspace problem, large deviations, subfactors, and beyond. This book puts a special emphasis on the relation of free probability to random matrices, but also touches upon the operator algebraic, combinatorial, and analytic aspects of the theory. The book serves as a combination textbook/research monograph, with self-contained chapters, exercises scattered throughout the text, and coverage of important ongoing progress of the theory. It will appeal to graduate students and all mathematicians interested in random matrices and free probability from the point of view of operator algebras, combinatorics, analytic functions, or applications in engineering and statistical physics.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 352 pp. Englisch.

Taschenbuch. Etat : Neu. Neuware -This volume provides protocols that revolve around three pillars of progress in the plant genomics field: genotypes, phenotypes, and the molecular processes in between. Chapters in Plant Genomics: Methods and Protocols are not restricted to the predominant model species Arabidopsis thaliana, hoping to encourage and facilitate other researchers to expand their research to other species. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 332 pp. Englisch.

Taschenbuch. Etat : Neu. Neuware -This book provides an easy-to-read, user-oriented introduction to mental models research and Mental Modeling TechnologyTM. Mental models are powerful influences human behavior. The book offers insight from the developers and most experienced application professionals of a widely proven methodology for understanding and influencing human judgment, decision making and behavior.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 292 pp. Englisch.

Taschenbuch. Etat : Neu. Neuware -This volume details methods for the analyses of specific lipid classes and lipidomics analyses of cells such as lymphocytes and oocytes. Lipidomics guides readers through chapters on direct-flow and chromatographic methods (SFC, UHPLC, HPTLC, ion-mobility); derivatization methods for lipids (amines, fatty aldehydes and ketones); TOF-SIMS imaging of lipids; and characterization of lipid transfer proteins. Additional chapters also provide an authoritative overview of lipidomics strategies and a detailed review of high-resolution mass spectrometric methods are included in this volume. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your own laboratory.Concise and easy-to-use, Lipidomics aims to ensure successful results in the further study of this vital field.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 260 pp. Englisch.

Taschenbuch. Etat : Neu. Neuware -This book is an informal autobiography by John West MD PhD. He obtained his medical degree in Adelaide, Australia and then spent 15 years mainly at the Royal Postgraduate Medical School, Hammersmith Hospital in London where he, with others, used radioactive oxygen-15 to make the first description of the uneven regional distribution of blood flow in the lung.In 1960-1961, he was a member of the Himalayan Scientific and Mountaineering Expedition led by Sir Edmund Hillary who had made the first ascent of Mt Everest 7 years before. During the expedition about 6 scientists spent up to three months at an altitude of 5800 m studying the effects of this very high altitude on human physiology.Because of his interests in the effects of gravity on the lung, Dr. West spent a year at the NASA Ames Research Center in Mountain View, California in 1967-1968. While there he submitted a proposal to NASA to measure pulmonary function of astronauts in space, and thiswas funded. Later, in 1981 he organized the American Medical Research Expedition to Everest during which the first measurements of human physiology on the summit, altitude 8848 m, were obtained. In the 1990¿s, Dr. West¿s team made the first comprehensive measurements of pulmonary function of astronauts in space using SpaceLab which was taken up in the Shuttle.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 164 pp. Englisch.

Taschenbuch. Etat : Neu. Neuware -This informative text details the many changes in everyday life as the result of injury, illness, or aging affecting the brain. Experts across brain-related fields trace mechanisms of conditions such as Parkinson¿s disease, TBI, and dementia as they impact regions of the brain, and resulting cognitive, emotional, sensory, and motor impairments as they contribute to deficits in personal and social functioning. In addition to symptoms and behaviors associated with insults to the brain (and the extent to which the brain can adapt or self-repair), chapters provide cogent examples of how societal and cultural expectations can shape the context and experience of disability. The book¿s focus on everyday activities brings new clarity to diverse links between symptoms and diagnosis, brain and behavior.Included in the coverage: The aging brain and changes in daily function. Stroke: impact on life and daily function. Traumatic brain injury (TBI) and the impact on daily life. Everyday life with cancer. Real-world impact of HIV-associated neurocognitive impairment. Disability and public policy in America. Living after brain changes, from the patient¿s perspective.Rich in empirical data and human insight, Changes in the Brain gives neuropsychologists, clinical psychologists, clinical social workers, and rehabilitation nurses a robust new understanding of the daily lives of patients, both in theory and in the real world.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 296 pp. Englisch.

Buch. Etat : Neu. Neuware -This volume offers detailed step-by-step instructions to allow beginners and experts alike to run appropriate copy number variants (CNV) detection software on a dataset of choice and discern between false positive noise and true positive CNV signals. Chapters guide readers through single nucleotide polymorphism (SNP) chips, optical mapping assembly techniques, and current open-source programs specializing in CNV detection. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Copy Number Variants: Methods and Protocols aims to provide guidance to Bioinformaticians and Molecular Biologists who are interested in identifying copy number variants (CNV) with a wide variety of experimental mediaHumana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 220 pp. Englisch.

Buch. Etat : Neu. Neuware -This volume discusses popular methods to achieve different types of mutagenesis and forward/reverse genetics in Medicago truncatula. Several studies on genetic control of developmental and metabolic processes in this model legume are also described. The chapters in this book cover topics such as Targeting Induced Local Lesions IN Genomes (TILLING), Fast Neutron Bombardment (FNB), Tnt1 insertional mutagenesis, Virus-Induced Gene Silencing (VIGS), stable inactivation of microRNAs in roots, gene editing by CRISPR-Cas9, etc. This book also contains reviews on the specific use of these techniques in functional studies on the genetic control of seed, leaf, root, nodule, floral and fruit development in M. truncatula. Written for the highly successful Methods in Molecular Biology series format, chapters contain the kind of detailed description and implementation advice needed to promote success in the lab.Cutting-edge and thorough, Functional Genomics in Medicago truncatula: Methods and Protocols is a valuable resource for anyone interested in learning more about this developing field.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 356 pp. Englisch.

Taschenbuch. Etat : Neu. Neuware -This volume provides laboratory protocols essential for studies on lysosomal biology. Chapters aim to guide researchers in their exploration of lysosomes, both under normal conditions and in pathological processes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Lysosome: Methods and Protocols aims to provided protocols that will guide and inspire further research and generate new insights into this fascinating organelle.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 328 pp. Englisch.

Taschenbuch. Etat : Neu. Neuware -This monograph presents a general mathematical theory for biological growth. It provides both a conceptual and a technical foundation for the understanding and analysis of problems arising in biology and physiology. The theory and methods are illustrated on a wide range of examples and applications.A process of extreme complexity, growth plays a fundamental role in many biological processes and is considered to be the hallmark of life itself. Its description has been one of the fundamental problems of life sciences, but until recently, it has not attracted much attention from mathematicians, physicists, and engineers. The author herein presents the first major technical monograph on the problem of growth since D¿Arcy Wentworth Thompson¿s 1917 book On Growth and Form.The emphasis of the book is on the proper mathematical formulation of growth kinematics and mechanics. Accordingly, the discussion proceeds in order of complexity and the book is divided into five parts. First, a general introduction on the problem of growth from a historical perspective is given. Then, basic concepts are introduced within the context of growth in filamentary structures. These ideas are then generalized to surfaces and membranes and eventually to the general case of volumetric growth. The book concludes with a discussion of open problems and outstanding challenges.Thoughtfully written and richly illustrated to be accessible to readers of varying interests and background, the text will appeal to life scientists, biophysicists, biomedical engineers, and applied mathematicians alike.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 668 pp. Englisch.

Taschenbuch. Etat : Neu. Neuware -This is the ideal book for anyone contemplating starting a career in, or shifting their career to, studying the dynamics that drive cancer progression and its response to therapy. Topics include the theory and population genetics of cancers, genetic diversity within tumors (intra-tumor heterogeneity), understanding how mutant clones expand in tissues, the role of cancer stem cells in the dynamics of tumors, the evolution of metastasis, and how to improve cancer therapy by addressing the evolution of cancers in response to our interventions. There are also chapters on the patterns of cancer susceptibility in humans due to a mismatch between our modern environment and the environment in which our ancestors evolved, as well as a chapter on the evolution of cancer suppression mechanisms that have evolved in different species, particularly the large long-lived animals like elephants and whales that are better at suppressing cancers than humans.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 272 pp. Englisch.

Buch. Etat : Neu. Neuware -This volume looks at the importance of V. cholerae as an infection agent, and explores the latest techniques used to study it. The chapters in this book cover topics such as the biology of V. cholerae; laboratory maintenance and culturing; various animals models developed to explain pathogenesis and factors for environmental survival; and holistic approaches that include proteomics, metabolomics, and high-throughput screening. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and authoritative, Vibrio cholerae: Methods and Protocols is a valuable resource for both novice microbiologists who want to focus their studies on V. cholerae, and experienced researchers and teachers who want to improve their knowledge and skills in this growing field.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 192 pp. Englisch.

Buch. Etat : Neu. Neuware -Cutting-edge and thorough, Plastids: Methods and Protocols is a valuable resource for students, engineers, and researchers who are interested in this evolving organelle and overall field.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 428 pp. Englisch.

Buch. Etat : Neu. Neuware -This detailed book expands upon the previous edition with a collection of methods for those performing experimental work on small GTPases of the Rho family. Split into four sections, the volume explores computational modeling and imaging procedures, biochemical methods related to post-translational modifications of Rho GTPases as well as some high throughput methods, functional assays that allow for monitoring the consequences of manipulating Rho GTPases in a variety of cell types and cell biology processes, and techniques specifically designed for studies in selected non-mammalian model organisms (zebrafish, social ameba, plants and algae). Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on trouble shooting and avoiding known pitfalls.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 436 pp. Englisch.

Buch. Etat : Neu. Neuware -This user-friendly book provides a range of classical and modern techniques for the study of photosynthesis in a manner accessible to a broad spectrum of researchers. Broken into four sections, it explores the measurement of physiological photosynthetic parameters, quantifying photosynthetic enzyme abundance and catalytic activity, visualizing cellular and sub-cellular phenotypes, and photosynthesis-inspired energy generation. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 368 pp. Englisch.