Development molecular methods detection par cromeans (23 résultats)
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Paperback. Etat : new. Paperback. Enteroviruses, hepatitis A virus (HAV) and other enteric viruses can survive wastewater treatment processes, even after chlorination, and are found in the final effluents. These viruses can be detected by cell culture techniques with observations for cytopathic effect (CPE). Recently molecular d…etection of viral nucleic acids has been used. Most viruses found in wastewater are RNA viruses and RT-PCR is a rapid and sensitive method to detect these single-strand RNA enteric viruses. This methodology does not distinguish between infectious and non-infectious viruses. Viruses inactivated in the treatment process can be detected but do not pose a public health threat. Methods are needed to quickly distinguish the infectious viruses from inactivated viruses, both of which may be present in effluents. In this project we investigated the use of a method that combines cell culture and molecular detection. If a sample contains viruses that replicate in cell culture even without CPE, the proof of replication can be demonstrated by the detection of a replicative form (RF) in cell culture that is only present during replication of infectious RNA viruses. Enteroviruses, hepatitis A virus (HAV) and other enteric viruses can survive wastewater treatment processes, even after chlorination, and are found in the final effluents. These viruses can be detected by cell culture techniques with observations for cytopathic effect (CPE). Recently molecular detection of viral nucleic acids has been used. Most viruses found in wastewater are RNA viruses and RT-PCR is a rapid and sensitive method to detect these single-strand RNA enteric viruses. This methodology does not distinguish between infectious and non-infectious viruses. Viruses inactivated in the treatment process can be detected but do not pose a public health threat. Methods are needed to quickly distinguish the infectious viruses from inactivated viruses, both of which may be present in effluents. In this project we investigated the use of a method that combines cell culture and molecular detection. If a sample contains viruses that replicate in cell culture even without CPE, the proof of replication can be demonstrated by the detection of a replicative form (RF) in cell culture that is only present during replication of infectious RNA viruses. A negative sense strand of RNA is generated from the viral positive strand virus, and these two are found primarily bound in a replicative form. This RNA was specifically detected by RT-PCR, including the positive and negative strands. This assay was developed and explored for the detection of low levels of CVB3 and HAV laboratory strains first and then for low levels of wild-type enteroviruses isolated from sewage samples. As few as four infectious units of wild-type enteroviruses, contained in 2.0 ml of water concentrate prepared from 600 ml of treated wastewater effluent, could be detected within two days. Treated wastewater effluents were collected weekly over an 18 months period, and viruses were concentrated with a developed method. About 7% of the final effluent wastewater samples were found positive for infectious enterovirus with the developed RF method, although only 1% of the samples were positive by presence of cytopathic effect. This method avoids the use of two complete cycles of cell culture to detect infectious virus, while confirming infectivity with a molecular method. Shipping may be from multiple locations in the US or from the UK, depending on stock availability.
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Vendeur : PBShop.store US, Wood Dale, IL, Etats-UnisPBShop.store US
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EUR 148,80
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PAP. Etat : New. New Book. Shipped from UK. Established seller since 2000.
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Vendeur : PBShop.store UK, Fairford, GLOS, Royaume-UniPBShop.store UK
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EUR 147,17
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PAP. Etat : New. New Book. Shipped from UK. Established seller since 2000.
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Vendeur : GreatBookPricesUK, Woodford Green, Royaume-UniGreatBookPricesUK
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EUR 147,15
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Etat : New.
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Vendeur : GreatBookPrices, Columbia, MD, Etats-UnisGreatBookPrices
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EUR 164,29
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Etat : As New. Unread book in perfect condition.
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Vendeur : Majestic Books, Hounslow, Royaume-UniMajestic Books
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Etat : New. pp. 80 Illus.
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Vendeur : Books Puddle, New York, NY, Etats-UnisBooks Puddle
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EUR 171,28
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Etat : New. pp. 80.
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Vendeur : Ria Christie Collections, Uxbridge, Royaume-UniRia Christie Collections
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EUR 161,42
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Etat : New. In.
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Vendeur : GreatBookPricesUK, Woodford Green, Royaume-UniGreatBookPricesUK
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Etat : As New. Unread book in perfect condition.
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Vendeur : Rarewaves USA, HEBRON, KY, Etats-UnisRarewaves USA
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EUR 186,06
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Paperback. Etat : New. Enteroviruses, hepatitis A virus (HAV) and other enteric viruses can survive wastewater treatment processes, even after chlorination, and are found in the final effluents. These viruses can be detected by cell culture techniques with observations for cytopathic effect (CPE). Recently molecular detection o…f viral nucleic acids has been used. Most viruses found in wastewater are RNA viruses and RT-PCR is a rapid and sensitive method to detect these single-strand RNA enteric viruses. This methodology does not distinguish between infectious and non-infectious viruses. Viruses inactivated in the treatment process can be detected but do not pose a public health threat. Methods are needed to quickly distinguish the infectious viruses from inactivated viruses, both of which may be present in effluents. In this project we investigated the use of a method that combines cell culture and molecular detection. If a sample contains viruses that replicate in cell culture even without CPE, the proof of replication can be demonstrated by the detection of a replicative form (RF) in cell culture that is only present during replication of infectious RNA viruses.
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Vendeur : Rarewaves.com USA, London, LONDO, Royaume-UniRarewaves.com USA
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EUR 186,13
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Paperback. Etat : New. Enteroviruses, hepatitis A virus (HAV) and other enteric viruses can survive wastewater treatment processes, even after chlorination, and are found in the final effluents. These viruses can be detected by cell culture techniques with observations for cytopathic effect (CPE). Recently molecular detection o…f viral nucleic acids has been used. Most viruses found in wastewater are RNA viruses and RT-PCR is a rapid and sensitive method to detect these single-strand RNA enteric viruses. This methodology does not distinguish between infectious and non-infectious viruses. Viruses inactivated in the treatment process can be detected but do not pose a public health threat. Methods are needed to quickly distinguish the infectious viruses from inactivated viruses, both of which may be present in effluents. In this project we investigated the use of a method that combines cell culture and molecular detection. If a sample contains viruses that replicate in cell culture even without CPE, the proof of replication can be demonstrated by the detection of a replicative form (RF) in cell culture that is only present during replication of infectious RNA viruses.
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Vendeur : THE SAINT BOOKSTORE, Southport, Royaume-UniTHE SAINT BOOKSTORE
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EUR 172,64
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Paperback / softback. Etat : New. New copy - Usually dispatched within 3 working days.
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Vendeur : Revaluation Books, Exeter, Royaume-UniRevaluation Books
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EUR 177,00
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Paperback. Etat : Brand New. illustrated edition. 78 pages. 10.75x8.25x0.25 inches. In Stock.
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Vendeur : Kennys Bookshop and Art Galleries Ltd., Galway, GY, IrlandeKennys Bookshop and Art Galleries Ltd.
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Etat : New. 2004. paperback. . . . . .
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Vendeur : Biblios, frankfurt am main, HESSE, AllemagneBiblios
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EUR 182,94
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Etat : New. pp. 80.
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Vendeur : moluna, Greven, Allemagnemoluna
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Etat : New. KlappentextEnteroviruses, hepatitis A virus (HAV) and other enteric viruses can survive wastewater treatment processes, even after chlorination, and are found in the final effluents. These viruses can be detected by cell culture techniq.
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Vendeur : Kennys Bookstore, Olney, MD, Etats-UnisKennys Bookstore
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EUR 222,01
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Etat : New. 2004. paperback. . . . . . Books ship from the US and Ireland.
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Vendeur : Rarewaves USA United, HEBRON, KY, Etats-UnisRarewaves USA United
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EUR 188,88
EUR 43,05 expéditionExpédition nationale : Etats-UnisQuantité disponible : 5 disponible(s)
Paperback. Etat : New. Enteroviruses, hepatitis A virus (HAV) and other enteric viruses can survive wastewater treatment processes, even after chlorination, and are found in the final effluents. These viruses can be detected by cell culture techniques with observations for cytopathic effect (CPE). Recently molecular detection o…f viral nucleic acids has been used. Most viruses found in wastewater are RNA viruses and RT-PCR is a rapid and sensitive method to detect these single-strand RNA enteric viruses. This methodology does not distinguish between infectious and non-infectious viruses. Viruses inactivated in the treatment process can be detected but do not pose a public health threat. Methods are needed to quickly distinguish the infectious viruses from inactivated viruses, both of which may be present in effluents. In this project we investigated the use of a method that combines cell culture and molecular detection. If a sample contains viruses that replicate in cell culture even without CPE, the proof of replication can be demonstrated by the detection of a replicative form (RF) in cell culture that is only present during replication of infectious RNA viruses.
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Vendeur : preigu, Osnabrück, Allemagnepreigu
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EUR 172,05
EUR 70,00 expéditionExpédition depuis Allemagne vers Etats-UnisQuantité disponible : 1 disponible(s)
Taschenbuch. Etat : Neu. Development of Molecular Methods for Detection of Infectious Viruses in Treated Wastewater | T. Cromeans | Taschenbuch | Werf Research Report | Kartoniert / Broschiert | Englisch | 2005 | Werf | EAN 9781843397229 | Verantwortliche Person für die EU: preigu GmbH & Co. KG, Lengericher Landstr. 19, 49078 Os…nabrück, mail[at]preigu[dot]de | Anbieter: preigu.
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Vendeur : AHA-BUCH GmbH, Einbeck, AllemagneAHA-BUCH GmbH
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EUR 183,42
EUR 61,12 expéditionExpédition depuis Allemagne vers Etats-UnisQuantité disponible : 2 disponible(s)
Taschenbuch. Etat : Neu. Neuware - Enteroviruses, hepatitis A virus (HAV) and other enteric viruses can survive wastewater treatment processes, even after chlorination, and are found in the final effluents. These viruses can be detected by cell culture techniques with observations for cytopathic effect (CPE). Recently molecular…detection of viral nucleic acids has been used. Most viruses found in wastewater are RNA viruses and RT-PCR is a rapid and sensitive method to detect these single-strand RNA enteric viruses. This methodology does not distinguish between infectious and non-infectious viruses. Viruses inactivated in the treatment process can be detected but do not pose a public health threat. Methods are needed to quickly distinguish the infectious viruses from inactivated viruses, both of which may be present in effluents. In this project we investigated the use of a method that combines cell culture and molecular detection. If a sample contains viruses that replicate in cell culture even without CPE, the proof of replication can be demonstrated by the detection of a replicative form (RF) in cell culture that is only present during replication of infectious RNA viruses. A negative sense strand of RNA is generated from the viral positive strand virus, and these two are found primarily bound in a replicative form. This RNA was specifically detected by RT-PCR, including the positive and negative strands. This assay was developed and explored for the detection of low levels of CVB3 and HAV laboratory strains first and then for low levels of wild-type enteroviruses isolated from sewage samples. As few as four infectious units of wild-type enteroviruses, contained in 2.0 ml of water concentrate prepared from 600 ml of treated wastewater effluent, could be detected within two days. Treated wastewater effluents were collected weekly over an 18 months period, and viruses were concentrated with a developed method. About 7% of the final effluent wastewater samples were found positive for infectious enterovirus with the developed RF method, although only 1% of the samples were positive by presence of cytopathic effect. This method avoids the use of two complete cycles of cell culture to detect infectious virus, while confirming infectivity with a molecular method.
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Vendeur : Rarewaves.com UK, London, Royaume-UniRarewaves.com UK
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EUR 181,95
EUR 75,65 expéditionExpédition depuis Royaume-Uni vers Etats-UnisQuantité disponible : 5 disponible(s)
Paperback. Etat : New. Enteroviruses, hepatitis A virus (HAV) and other enteric viruses can survive wastewater treatment processes, even after chlorination, and are found in the final effluents. These viruses can be detected by cell culture techniques with observations for cytopathic effect (CPE). Recently molecular detection o…f viral nucleic acids has been used. Most viruses found in wastewater are RNA viruses and RT-PCR is a rapid and sensitive method to detect these single-strand RNA enteric viruses. This methodology does not distinguish between infectious and non-infectious viruses. Viruses inactivated in the treatment process can be detected but do not pose a public health threat. Methods are needed to quickly distinguish the infectious viruses from inactivated viruses, both of which may be present in effluents. In this project we investigated the use of a method that combines cell culture and molecular detection. If a sample contains viruses that replicate in cell culture even without CPE, the proof of replication can be demonstrated by the detection of a replicative form (RF) in cell culture that is only present during replication of infectious RNA viruses.
- Couverture souple
Vendeur : AussieBookSeller, Truganina, VIC, AustralieAussieBookSeller
Contacter le vendeurVendeur avec une évaluation de 5 étoilesEtat: Neuf
EUR 245,81
EUR 31,86 expéditionExpédition depuis Australie vers Etats-UnisQuantité disponible : 1 disponible(s)
Paperback. Etat : new. Paperback. Enteroviruses, hepatitis A virus (HAV) and other enteric viruses can survive wastewater treatment processes, even after chlorination, and are found in the final effluents. These viruses can be detected by cell culture techniques with observations for cytopathic effect (CPE). Recently molecular d…etection of viral nucleic acids has been used. Most viruses found in wastewater are RNA viruses and RT-PCR is a rapid and sensitive method to detect these single-strand RNA enteric viruses. This methodology does not distinguish between infectious and non-infectious viruses. Viruses inactivated in the treatment process can be detected but do not pose a public health threat. Methods are needed to quickly distinguish the infectious viruses from inactivated viruses, both of which may be present in effluents. In this project we investigated the use of a method that combines cell culture and molecular detection. If a sample contains viruses that replicate in cell culture even without CPE, the proof of replication can be demonstrated by the detection of a replicative form (RF) in cell culture that is only present during replication of infectious RNA viruses. Enteroviruses, hepatitis A virus (HAV) and other enteric viruses can survive wastewater treatment processes, even after chlorination, and are found in the final effluents. These viruses can be detected by cell culture techniques with observations for cytopathic effect (CPE). Recently molecular detection of viral nucleic acids has been used. Most viruses found in wastewater are RNA viruses and RT-PCR is a rapid and sensitive method to detect these single-strand RNA enteric viruses. This methodology does not distinguish between infectious and non-infectious viruses. Viruses inactivated in the treatment process can be detected but do not pose a public health threat. Methods are needed to quickly distinguish the infectious viruses from inactivated viruses, both of which may be present in effluents. In this project we investigated the use of a method that combines cell culture and molecular detection. If a sample contains viruses that replicate in cell culture even without CPE, the proof of replication can be demonstrated by the detection of a replicative form (RF) in cell culture that is only present during replication of infectious RNA viruses. A negative sense strand of RNA is generated from the viral positive strand virus, and these two are found primarily bound in a replicative form. This RNA was specifically detected by RT-PCR, including the positive and negative strands. This assay was developed and explored for the detection of low levels of CVB3 and HAV laboratory strains first and then for low levels of wild-type enteroviruses isolated from sewage samples. As few as four infectious units of wild-type enteroviruses, contained in 2.0 ml of water concentrate prepared from 600 ml of treated wastewater effluent, could be detected within two days. Treated wastewater effluents were collected weekly over an 18 months period, and viruses were concentrated with a developed method. About 7% of the final effluent wastewater samples were found positive for infectious enterovirus with the developed RF method, although only 1% of the samples were positive by presence of cytopathic effect. This method avoids the use of two complete cycles of cell culture to detect infectious virus, while confirming infectivity with a molecular method. Shipping may be from our Sydney, NSW warehouse or from our UK or US warehouse, depending on stock availability.
